Interspecific differences between the DNA restriction profiles of canine adenoviruses

1984 ◽  
Vol 40 (5) ◽  
pp. 482-482 ◽  
Author(s):  
C. Hamelin ◽  
G. Marsolais ◽  
R. Assaf
Keyword(s):  
Interspecific Differences ◽  
Restriction Profiles ◽  
Dna Restriction

scholarly journals Differentiation of polyvalent bacteriophages specific to uropathogenic Proteus mirabilis strains based on the host range pattern and RFLP.

2016 ◽  
Vol 63 (2) ◽  
Author(s):  
Agnieszka Maszewska ◽  
Ewelina Wójcik ◽  
Aneta Ciurzyńska ◽  
Arkadiusz Wojtasik ◽  
Iwona Piątkowska ◽  
...  
Keyword(s):  
Host Range ◽  
Similarity Index ◽  
Rflp Analysis ◽  
Range Analysis ◽  
Restriction Profiles ◽  
Infection Treatment ◽  
Bacterial Lawn ◽  
Dna Restriction ◽  
Complete Lysis ◽  
Tract Infections

Urinary tract infections (UTIs) caused by P. mirabilis are difficult to cure because of the increasing antimicrobial resistance of these bacteria. Phage therapy is proposed as an alternative infection treatment. The aim of this study was to isolate and differentiate uropathogenic P. mirabilis strain specific polyvalent bacteriophages producing polysaccharide depolymerases (PDs). 51 specific phages were obtained. The plaques of 29 bacteriophages were surrounded by halos, which indicated that they produced PDs. The host range analysis showed that, except phages 58B and 58C, the phage host range profiles differed from each other. Phages 35 and 45 infected all P. mirabilis strains tested. Another 10 phages lysed more than 90% of isolates. Among these phages, 65A, 70, 66 and 66A caused a complete lysis of the bacterial lawn formed by 62% to 78% of strains. Additionally, phages 39A and 70 probably produced PDs. The phages' DNA restriction fragment length polymorphism (RFLP) analysis demonstrated that genomes of 51 isolated phages represented 34 different restriction profiles. DNA of phage 58A seemed to be resistant to selected EcoRV endonuclease. The 33 RFLP-EcoRV profiles showed a Dice similarity index of 38.8%. 22 RFLP patterns were obtained from single phage isolates. The remaining 12 restriction profiles consisted of 2 to 4 viruses. The results obtained from phage characterization based on the pattern of phage host range in combination with the RFLP method enabled effective differentiation of the studied phages and selection of PD producing polyvalent phages for further study.

Interactions of temperate bacteriophages ofStreptococcus salivariussubsp.thermophiluswith lysogenic indicators affect phage DNA restriction patterns and host ranges

1993 ◽  
Vol 60 (3) ◽  
pp. 385-399 ◽  
Author(s):  
Blandine Fayard ◽  
Moritz Haefliger ◽  
Jean-Pierre Accolas
Keyword(s):  
Mitomycin C ◽  
International Committee ◽  
Cross Hybridization ◽  
Clear Cut ◽  
Restriction Profiles ◽  
Phage Dna ◽  
Streptococcal Species ◽  
Dna Restriction ◽  
Group B

SummaryAfter treatment with mitomycin C, 12 of the 120 strains of the French Collection of Lactic Acid Bacteria (CNRZ) belonging to the speciesStreptococcus salivariussubsp.thermophiluswere found to be inducible and lysogenic. The corresponding temperate phages were multiplied on indicator strains and further characterized. It is noteworthy that some of the indicators were themselves lysogenic. The temperate phages belonged to Bradley's group B, or to theSiphoviridaefamily of the International Committee on Taxonomy of Viruses. Seven of them were shared among the two protein profiles previously established for virulent phages ofStr. salivariussubsp.thermophilus. Seven different DNA restriction profiles were found for ten native temperate phages examined just after mitomycin C induction. Genome sizes varied from 40 to 45 kb and were classified into four related homology groups by DNA cross-hybridization, but there was no clear-cut relationship with the protein clusters previously shown. DNA homology with representatives of virulent phages was also found. The DNA restriction profiles of seven native temperate phages, examined just after mitomycin C induction of the lysogenic hosts, were noticeably different from those of the corresponding phages once they had been propagated on lysogenic indicators. Moreover, the host range of the latter phages was extended to a greater number of sensitive strains. The possible role of lysogenic strains ofStr. salivariussubsp.thermophilusin the occurrence of phage outbreaks affecting this dairy streptococcal species is discussed.

DNA Restriction Profiles of Nontypable Group B Streptococcal Clinical Isolates

1997 ◽  
pp. 343-346 ◽  
Author(s):  
Patricia Ferrieri ◽  
David S. Cho ◽  
Carol Livdahl ◽  
Craig E. Rubens ◽  
Aurea E. Flores
Keyword(s):  
Clinical Isolates ◽  
Restriction Profiles ◽  
Dna Restriction ◽  
Group B

Recognition of Leuconostoc oenos strains by the use of DNA restriction profiles

1993 ◽  
Vol 39 (4-5) ◽  
pp. 547-552 ◽  
Author(s):  
M. Lamoureux ◽  
H. Pr�vost ◽  
J. F. Cavin ◽  
C. Divi�s
Keyword(s):  
Leuconostoc Oenos ◽  
Restriction Profiles ◽  
Dna Restriction

Long-term in vitro Storage Technique of Valuable Birch Genotypes and Plant Production on its Basis

2019 ◽  
pp. 57-67
Author(s):  
T.M. Tabatskaya ◽  
N.I. Vnukova
Keyword(s):  
Plant Production ◽  
High Survival ◽  
Ex Vitro ◽  
Regenerative Capacity ◽  
In Vitro Storage ◽  
Interspecific Differences ◽  
Regenerated Plants ◽  
Growing Conditions

A technique for the long-term (up to 27 years) in vitro storage of valuable birch genotypes under normal (25 °C, 2.0 klx, 16-h day and 8-h night) and low temperature (4 °C, 0.5 klx, 6-h day and 18-h night) growing conditions on hormone-free media has been described. The study explored for the first time the influence of different strategies to store the clones of Betula pubescens and B. pendula var. сarelica (6 genotypes) on the regenerative capacity of collection samples, adaptive potential of regenerated plants and plant production by the in vitro and ex vitro techniques. It was established that both storage strategies provided a persistently high survival rate (82-100%) and regenerative capacity of in vitro shoots (the multiplication coefficient of 4.2-6.3 and rhizogenic activity of 90-100%). The clones retained their characteristics of height growth under the in vitro and ex vitro conditions, and demonstrated intraclonal homogeneity and lack of signs of somaclonal variability. The plants showed substantial interspecific differences at the stage of multiplication and transfer to the greenhouse. The highest percentage of acclimated plants (75-98% depending on the clone genotype) was obtained after planting of micro plants straight in the greenhouse, which simplified the technology and made plant production less costly. long-term in vitro storage, birch, species, genotype, micropropagation, ex vitro adaptation, plant material

Altitudinal and Interspecific Differences in the Rehydration Abilities of Puerto Rican Frogs (Eleutherodactylus)

1982 ◽  
Vol 55 (2) ◽  
pp. 130-136 ◽  
Author(s):  
Fredrica Van Berkum ◽  
F. Harvey Pough ◽  
Margaret M. Stewart ◽  
Peter F. Brussard
Keyword(s):  
Puerto Rican ◽  
Interspecific Differences

scholarly journals Selective pollination by fungus gnats potentially functions as an alternative reproductive isolation among five Arisaema species

2020 ◽  
Author(s):  
Tetsuya K Matsumoto ◽  
Muneto Hirobe ◽  
Masahiro Sueyoshi ◽  
Yuko Miyazaki
Keyword(s):  
Reproductive Isolation ◽  
Altitudinal Gradient ◽  
Flowering Phenology ◽  
Altitudinal Distribution ◽  
Interspecific Differences ◽  
Flower Visitation ◽  
Fungus Gnats ◽  
Floral Visitor ◽  
Floral Visitors ◽  
Experimental Plots

Abstract Background and Aims Interspecific difference in pollinators (pollinator isolation) is important for reproductive isolation in flowering plants. Species-specific pollination by fungus gnats has been discovered in several plant taxa, suggesting that they can contribute to reproductive isolation. Nevertheless, their contribution has not been studied in detail, partly because they are too small for field observations during flower visitation. To quantify their flower visitation, we used the genus Arisaema (Araceae) because the pitcher-like spathe of Arisaema can trap all floral visitors. Methods We evaluated floral visitor assemblage in an altitudinal gradient including five Arisaema species. We also examined interspecific differences in altitudinal distribution (geographic isolation) and flowering phenology (phenological isolation). To exclude the effect of interspecific differences in altitudinal distribution on floral visitor assemblage, we established 10 experimental plots including the five Arisaema species on high- and low-altitude areas and collected floral visitors. We also collected floral visitors in three additional sites. Finally, we estimated the strength and contribution of these three reproductive barriers using the unified formula for reproductive isolation. Key Results Each Arisaema species selectively attracted different fungus gnats in the altitudinal gradient, experimental plots, and additional sites. Altitudinal distribution and flowering phenology differed among the five Arisaema species, whereas the strength of geographic and phenological isolations were distinctly weaker than those in pollinator isolation. Nevertheless, the absolute contribution of pollinator isolation to total reproductive isolation was weaker than geographic and phenological isolations, because pollinator isolation functions after the two early-acting barriers in plant life history. Conclusions Our results suggest that selective pollination by fungus gnats potentially contributes to reproductive isolation. Since geographic and phenological isolations can be disrupted by habitat disturbance and interannual climate change, the strong and stable pollinator isolation might compensate for the weakened early-acting barriers as an alternative reproductive isolation among the five Arisaema species.

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