Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles

1987 ◽  
Vol 75 (1) ◽  
pp. 51-58 ◽  
Author(s):  
A. Vardi ◽  
A. Breiman ◽  
E. Galun
Keyword(s):  
Mitochondrial Dna ◽  
Protoplast Fusion ◽  
Restriction Profiles ◽  
Dna Restriction

Characterization of Cloned Mitochondrial DNA from the Teleost Fundulus heteroclitus and its Usefulness as an Interspecies Hybridization Probe

1986 ◽  
Vol 43 (10) ◽  
pp. 1866-1872 ◽  
Author(s):  
Lucia Irene González-Villaseñor ◽  
Amanda M. Burkhoff ◽  
Víctor Corces ◽  
Dennis A. Powers
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Fundulus Heteroclitus ◽  
Staining Technique ◽  
Hybridization Probe ◽  
Cross Hybridization ◽  
Restriction Patterns ◽  
Ethidium Bromide Staining ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Analysis of mitochondrial DNA endonuclease restriction patterns is a powerful tool for studying related species and variation within species. The ethidium bromide staining technique has limited the number of digestions of mitochondrial DNA per individual. Because 32P-end-labeling also imposes severe limitations, we have resorted to cloning the fish (Fundulus heteroclitus) mitochondrial genome in the lambda replacement vector EMBL-3. The clone was used as a radioactive probe via Southern blotting to detect mitochondrial DNA restriction fragments obtained by multiple restriction endonuclease digestions from small amounts of tissue. This technique offers much greater sensitivity than ethidium bromide staining. Moreover, it eliminates the expense and time to obtain highly purified mitochondrial DNA for the 32P-end-labeling procedure. It is also useful when the mtDNA is prepared from frozen tissue which has been a problem with the 32P-end-labeling technique. Because the cloned mitochondrial DNA has a high degree of cross-hybridization with the mitochondrial DNA of certain other fishes, it can be used to probe the mitochondrial DNA restriction patterns of a variety of fish species. However, its usefulness is restricted by the degree of relatedness to the species being cloned.

Assignment of two mitochondrially synthesized polypeptides to human mitochondrial DNA and their use in the study of intracellular mitochondrial interaction

1982 ◽  
Vol 2 (1) ◽  
pp. 30-41
Author(s):  
N A Oliver ◽  
D C Wallace
Keyword(s):  
Mitochondrial Dna ◽  
Restriction Site ◽  
Mitochondrial Protein ◽  
Mitochondrial Protein Synthesis ◽  
Human Mitochondrial Dna ◽  
Ht1080 Cells ◽  
Mitochondrial Dnas ◽  
A Cell ◽  
Dna Restriction ◽  
Restriction Site Polymorphisms

Two mitochondrially synthesized marker polypeptides, MV-1 and MV-2, were found in human HeLa and HT1080 cells. These were assigned to the mitochondrial DNA in HeLa-HT1080 cybrids and hybrids by demonstrating their linkage to cytoplasmic genetic markers. These markers include mitochondrial DNA restriction site polymorphisms and resistance to chloramphenicol, an inhibitor of mitochondrial protein synthesis. In the absence of chloramphenicol, the expression of MV-1 and MV-2 in cybrids and hybrids was found to be directly proportional to the ratio of the parental mitochondrial DNAs. In the presence of chloramphenicol, the marker polypeptide linked to the chloramphenicol-sensitive mitochondrial DNA continued to be expressed. This demonstrated that resistant and sensitive mitochondrial DNAs can cooperate within a cell for gene expression and that the CAP-resistant allele was dominant or codominant to sensitive. Such cooperation suggests that mitochondrial DNAs can be exchanged between mitochondria.

Analysis of restriction profiles of mitochondrial DNA from Sporothrix schenckii

2001 ◽  
Vol 39 (5) ◽  
pp. 439-444 ◽  
Author(s):  
M. Mora-Cabrera ◽  
R. A. Alonso ◽  
R. Ulloa-Arvizu ◽  
H. Torres-Guerrero
Keyword(s):  
Mitochondrial Dna ◽  
Sporothrix Schenckii ◽  
Restriction Profiles

Inheritance of mitochondrial DNA in interspecific crosses of Piceaglauca and Piceaomorika

1996 ◽  
Vol 26 (3) ◽  
pp. 428-432 ◽  
Author(s):  
Andrew J. David ◽  
Daniel E. Keathley
Keyword(s):  
Mitochondrial Dna ◽  
Interspecific Hybrids ◽  
Maternal Inheritance ◽  
White Spruce ◽  
Mitochondrial Genes ◽  
Interspecific Crosses ◽  
Length Polymorphisms ◽  
Dna Restriction ◽  
Total Dna ◽  
Serbian Spruce

Fifteen interspecific hybrids of Serbian spruce (Piceaomorika (Panc) Purkyne) and white spruce (Piceaglauca (Moench) Voss) representing five separate crosses, including reciprocals, were used to demonstrate maternal inheritance of mitochondrial DNA. Total DNA was extracted from foliage samples of Serbian spruce (S), white spruce (W), and both S(♂) × W(♀) and W(♂) × S(♀) hybrids, digested and probed with one of two maize mitochondrial genes, ATPaseα or COXII. ATPaseα generated diagnostic Serbian and white spruce genotypes for all five enzymes tested, while COXII differentiated between the two species for four of five enzymes. Maternal inheritance was indicated in all hybrids for every diagnostic enzyme–probe combination. No paternal or nonparental bands were detected. A dilution experiment indicated that the Serbian and white spruce mitochondrial DNA restriction fragment length polymorphisms could be detected in as little as 60 and 500 ng of total DNA, respectively. It appears that the mechanism that controls the inheritance of mitochondria in Picea is still functional in wide interspecific crosses.

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