Recognition of Leuconostoc oenos strains by the use of DNA restriction profiles

1993 ◽  
Vol 39 (4-5) ◽  
pp. 547-552 ◽  
Author(s):  
M. Lamoureux ◽  
H. Pr�vost ◽  
J. F. Cavin ◽  
C. Divi�s
Keyword(s):  
Leuconostoc Oenos ◽  
Restriction Profiles ◽  
Dna Restriction

scholarly journals Differentiation of polyvalent bacteriophages specific to uropathogenic Proteus mirabilis strains based on the host range pattern and RFLP.

2016 ◽  
Vol 63 (2) ◽  
Author(s):  
Agnieszka Maszewska ◽  
Ewelina Wójcik ◽  
Aneta Ciurzyńska ◽  
Arkadiusz Wojtasik ◽  
Iwona Piątkowska ◽  
...  
Keyword(s):  
Host Range ◽  
Similarity Index ◽  
Rflp Analysis ◽  
Range Analysis ◽  
Restriction Profiles ◽  
Infection Treatment ◽  
Bacterial Lawn ◽  
Dna Restriction ◽  
Complete Lysis ◽  
Tract Infections

Urinary tract infections (UTIs) caused by P. mirabilis are difficult to cure because of the increasing antimicrobial resistance of these bacteria. Phage therapy is proposed as an alternative infection treatment. The aim of this study was to isolate and differentiate uropathogenic P. mirabilis strain specific polyvalent bacteriophages producing polysaccharide depolymerases (PDs). 51 specific phages were obtained. The plaques of 29 bacteriophages were surrounded by halos, which indicated that they produced PDs. The host range analysis showed that, except phages 58B and 58C, the phage host range profiles differed from each other. Phages 35 and 45 infected all P. mirabilis strains tested. Another 10 phages lysed more than 90% of isolates. Among these phages, 65A, 70, 66 and 66A caused a complete lysis of the bacterial lawn formed by 62% to 78% of strains. Additionally, phages 39A and 70 probably produced PDs. The phages' DNA restriction fragment length polymorphism (RFLP) analysis demonstrated that genomes of 51 isolated phages represented 34 different restriction profiles. DNA of phage 58A seemed to be resistant to selected EcoRV endonuclease. The 33 RFLP-EcoRV profiles showed a Dice similarity index of 38.8%. 22 RFLP patterns were obtained from single phage isolates. The remaining 12 restriction profiles consisted of 2 to 4 viruses. The results obtained from phage characterization based on the pattern of phage host range in combination with the RFLP method enabled effective differentiation of the studied phages and selection of PD producing polyvalent phages for further study.

Interactions of temperate bacteriophages ofStreptococcus salivariussubsp.thermophiluswith lysogenic indicators affect phage DNA restriction patterns and host ranges

1993 ◽  
Vol 60 (3) ◽  
pp. 385-399 ◽  
Author(s):  
Blandine Fayard ◽  
Moritz Haefliger ◽  
Jean-Pierre Accolas
Keyword(s):  
Mitomycin C ◽  
International Committee ◽  
Cross Hybridization ◽  
Clear Cut ◽  
Restriction Profiles ◽  
Phage Dna ◽  
Streptococcal Species ◽  
Dna Restriction ◽  
Group B

SummaryAfter treatment with mitomycin C, 12 of the 120 strains of the French Collection of Lactic Acid Bacteria (CNRZ) belonging to the speciesStreptococcus salivariussubsp.thermophiluswere found to be inducible and lysogenic. The corresponding temperate phages were multiplied on indicator strains and further characterized. It is noteworthy that some of the indicators were themselves lysogenic. The temperate phages belonged to Bradley's group B, or to theSiphoviridaefamily of the International Committee on Taxonomy of Viruses. Seven of them were shared among the two protein profiles previously established for virulent phages ofStr. salivariussubsp.thermophilus. Seven different DNA restriction profiles were found for ten native temperate phages examined just after mitomycin C induction. Genome sizes varied from 40 to 45 kb and were classified into four related homology groups by DNA cross-hybridization, but there was no clear-cut relationship with the protein clusters previously shown. DNA homology with representatives of virulent phages was also found. The DNA restriction profiles of seven native temperate phages, examined just after mitomycin C induction of the lysogenic hosts, were noticeably different from those of the corresponding phages once they had been propagated on lysogenic indicators. Moreover, the host range of the latter phages was extended to a greater number of sensitive strains. The possible role of lysogenic strains ofStr. salivariussubsp.thermophilusin the occurrence of phage outbreaks affecting this dairy streptococcal species is discussed.

DNA Restriction Profiles of Nontypable Group B Streptococcal Clinical Isolates

1997 ◽  
pp. 343-346 ◽  
Author(s):  
Patricia Ferrieri ◽  
David S. Cho ◽  
Carol Livdahl ◽  
Craig E. Rubens ◽  
Aurea E. Flores
Keyword(s):  
Clinical Isolates ◽  
Restriction Profiles ◽  
Dna Restriction ◽  
Group B

Characterization of Cloned Mitochondrial DNA from the Teleost Fundulus heteroclitus and its Usefulness as an Interspecies Hybridization Probe

1986 ◽  
Vol 43 (10) ◽  
pp. 1866-1872 ◽  
Author(s):  
Lucia Irene González-Villaseñor ◽  
Amanda M. Burkhoff ◽  
Víctor Corces ◽  
Dennis A. Powers
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Fundulus Heteroclitus ◽  
Staining Technique ◽  
Hybridization Probe ◽  
Cross Hybridization ◽  
Restriction Patterns ◽  
Ethidium Bromide Staining ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Analysis of mitochondrial DNA endonuclease restriction patterns is a powerful tool for studying related species and variation within species. The ethidium bromide staining technique has limited the number of digestions of mitochondrial DNA per individual. Because 32P-end-labeling also imposes severe limitations, we have resorted to cloning the fish (Fundulus heteroclitus) mitochondrial genome in the lambda replacement vector EMBL-3. The clone was used as a radioactive probe via Southern blotting to detect mitochondrial DNA restriction fragments obtained by multiple restriction endonuclease digestions from small amounts of tissue. This technique offers much greater sensitivity than ethidium bromide staining. Moreover, it eliminates the expense and time to obtain highly purified mitochondrial DNA for the 32P-end-labeling procedure. It is also useful when the mtDNA is prepared from frozen tissue which has been a problem with the 32P-end-labeling technique. Because the cloned mitochondrial DNA has a high degree of cross-hybridization with the mitochondrial DNA of certain other fishes, it can be used to probe the mitochondrial DNA restriction patterns of a variety of fish species. However, its usefulness is restricted by the degree of relatedness to the species being cloned.

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