Stereotopography of the prolactin cells of the rat pituitary gland

1974 ◽  
Vol 30 (12) ◽  
pp. 1444-1446 ◽  
Author(s):  
J. B. Warchol ◽  
M. Shiino ◽  
E. G. Rennels
Keyword(s):  
Pituitary Gland ◽  
Prolactin Cells ◽  
Rat Pituitary

scholarly journals CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 558
Author(s):  
ZeWen Yu ◽  
WenZhi Ren ◽  
Tian Wang ◽  
WeiDi Zhang ◽  
ChangJiang Wang ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Inhibitory Effect ◽  
Pituitary Cells ◽  
Sequencing Analysis ◽  
Hormone Regulation ◽  
Gh Secretion ◽  
Qrt Pcr ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

CircRNAs have been identified to be expressed differently and stably in numerous species and tissues, but their functions in growth hormone (GH) secretion are still largely unknown. In summary, we have revealed a circRNA-miRNA-mRNA network that may play a biological role in the rat pituitary gland. First, we verified the chromosome location information of circAgtpbp1 according to sequencing analysis. The circAgtpbp1 characteristics were authenticated through PCR, qRT–PCR, treating with RNase and fluorescent in situ hybridization (FISH). Second, we detected the expression pattern of circAgtpbp1 in the rat anterior pituitary by qRT–PCR. We also designed circAgtpbp1 siRNA and constructed overexpression plasmid to evaluate the effect of circAgtpbp1 function on GH secretion by qRT–PCR, ELISA and Western blot. CircAgtpbp1 is a stable, truly circular molecule. We found that circAgtpbp1 interacted with miR-543-5p and can regulate GH secretion in pituitary cells through a circAgtpbp1-miR-543-5p-GH axis. Overall, the evidence generated by our study suggests that circAgtpbp1 can act as a sponge of miR-543-5p to reduce the inhibitory effect of miR-543-5p on Gh1 and further promote GH secretion. These findings expand our existing knowledge on the mechanisms of hormone regulation in the pituitary gland.

Synaptophysin Immunoreactivity in the Aging Rat Pituitary Gland

1997 ◽  
Vol 43 (6) ◽  
pp. 561-564 ◽  
Author(s):  
L.C Saland ◽  
A Apodaca ◽  
D Ramirez ◽  
V Hernandez ◽  
J Gaddy ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Rat Pituitary ◽  
Aging Rat

DISTRIBUTION OF NEUROSECRETORY GRANULES AMONG THE ANATOMICAL COMPARTMENTS OF THE NEUROSECRETORY PROCESSES OF THE PITUITARY GLAND: A QUANTITATIVE ULTRASTRUCTURAL APPROACH TO HORMONE STORAGE IN THE NEURAL LOBE

1976 ◽  
Vol 68 (2) ◽  
pp. 225-NP ◽  
Author(s):  
J. F. MORRIS
Keyword(s):  
Basement Membrane ◽  
Pituitary Gland ◽  
Neurosecretory Granules ◽  
Neural Lobe ◽  
Releasable Pool ◽  
Readily Releasable Pool ◽  
Rat Pituitary ◽  
Axonal Swellings ◽  
Granule Release

SUMMARY The distribution of neurosecretory granules in various anatomical compartments of neurosecretory axons of the neural lobe of the rat pituitary has been studied. Apart from the most anterior tip of the gland, where granules are largely restricted to undilated axons and a few 'swellings', the proportional compartmental storage of granules is essentially homogeneous for the rest of the gland: 13% of granules are found in undilated axons, 31% in axonal 'endings' (which contain microvesicles and abut the basement membrane) and 55% in axonal 'swellings' (which are devoid of significant numbers of microvesicles). These values indicate that the 'endings' contain a much greater proportion of the total number of granules stored in the neural lobe than would be predicted if the readily releasable pool of hormone were composed of all the granules in the 'endings'. Some further constraint on granule release either physiological or anatomical (e.g. the position of the granule in relation to the plasmalemma of the 'ending') must be operative.

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