scholarly journals Estrogen Receptors are Involved in Xenoestrogen Induction of Growth Hormone in the Rat Pituitary Gland

2009 ◽  
Vol 55 (2) ◽  
pp. 206-213 ◽  
Author(s):  
Vu Hoang DANG ◽  
Kyung-Chul CHOI ◽  
Eui-Bae JEUNG
1985 ◽  
Vol 101 (1) ◽  
pp. 305-311 ◽  
Author(s):  
P Kristensen ◽  
L S Nielsen ◽  
J Grøndahl-Hansen ◽  
P B Andresen ◽  
L I Larsson ◽  
...  

We immunocytochemically stained rat pituitary glands using antibodies against plasminogen activators of the tissue type (t-PA) and the urokinase type (u-PA). A large population of endocrine cells in the anterior lobe of the gland displayed intense cytoplasmic immunoreactivity with anti-t-PA. In some areas of the intermediate lobe we found a weak staining, and we observed weakly staining granular structures in the posterior lobe. Controls included absorption of the antibodies with highly purified t-PA. In addition, SDS PAGE followed by immunoblotting of pituitary gland extracts revealed only one band with an electrophoretic mobility similar to that of t-PA when stained with anti-t-PA IgG. No u-PA immunoreactivity was detected in the rat pituitary gland. Sequential staining experiments using antibodies against growth hormone and t-PA demonstrated that the t-PA-immunoreactive cells constitute a large subpopulation of the growth hormone-containing cells. These findings represent the first direct evidence for the presence of t-PA in cell types other than endothelial cells in the intact normal organism. In this article we discuss the implications of the results for a possible role of t-PA in the posttranslational processing of prohormones.


1993 ◽  
Vol 5 (5) ◽  
pp. 553-556 ◽  
Author(s):  
Pablo-Manuel Dobado-Berrios ◽  
Songuyn Li ◽  
Ester Garcia Yebenes ◽  
Georges Pelletier

Endocrinology ◽  
1967 ◽  
Vol 81 (2) ◽  
pp. 195-204 ◽  
Author(s):  
CLIFFORD A. BIRGE ◽  
GLENN T. PEAKE ◽  
IDA K. MARIZ ◽  
WILLIAM H. DAUGHADAY

1998 ◽  
Vol 251 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Masayuki Hara ◽  
Damon C. Herbert ◽  
Takashi Taniguchi ◽  
Atsuhiko Hattori ◽  
Ritsuko Ohtani-Kaneko ◽  
...  

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