Localization of potato leafroll virus in leaves of secondarily-infected potato plants

1995 ◽  
Vol 101 (5) ◽  
pp. 567-571 ◽  
Author(s):  
J. F. J. M. van den Heuvel ◽  
C. M. de Blank ◽  
D. Peters ◽  
J. W. M. van Lent
Keyword(s):  
Potato Leafroll Virus ◽  
Potato Plants ◽  
Leafroll Virus

Infection of potato plants with potato leafroll virus changes attraction and feeding behaviour of Myzus persicae

2007 ◽  
Vol 125 (2) ◽  
pp. 135-144 ◽  
Author(s):  
A. E. Alvarez ◽  
E. Garzo ◽  
M. Verbeek ◽  
B. Vosman ◽  
M. Dicke ◽  
...  
Keyword(s):  
Myzus Persicae ◽  
Feeding Behaviour ◽  
Potato Leafroll Virus ◽  
Potato Plants ◽  
Leafroll Virus

scholarly journals Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Priyanka Kumari ◽  
Jitesh Kumar ◽  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Movement Protein ◽  
Control Plant ◽  
Plant Viruses ◽  
Potato Leafroll Virus ◽  
Plant Diseases ◽  
World Population ◽  
Genome Replication ◽  
Potato Plants ◽  
Leafroll Virus

AbstractViruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of virion particles is completed inside the host plant, mature virions or sometimes naked viral genomes spread cell-to-cell through plasmodesmata by interacting with the virus-encoded movement protein (MP). We used the RNAi approach to suppress MP gene expression, which in turn prevented potato leafroll virus (PLRV) systemic infection in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with MP siRNA constructs exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of MP gene expression is an efficient method for generating PLRV-resistant potato plants. Further, we identified novel ATPase motifs in MP that may be involved in DNA binding and translocation through plasmodesmata. We also showed that the ATPase activity of MP was stimulated in the presence of DNA/RNA. Overall, our findings provide a robust technology to generate PLRV-resistant potato plants, which can be extended to other species. Moreover, this approach also contributes to the study of genome translocation mechanisms of plant viruses.

Acquisition of potato leafroll virus byMyzus persicae from secondarily-infected potato plants of different genotypes

1993 ◽  
Vol 36 (2) ◽  
pp. 89-96 ◽  
Author(s):  
J. F. J. M. van den Heuvel ◽  
J. A. A. M. Dirven ◽  
G. J. van Os ◽  
D. Peters
Keyword(s):  
Potato Leafroll Virus ◽  
Potato Plants ◽  
Leafroll Virus

scholarly journals First Report of Solanum sarrachoides (Hairy Nightshade) as an Important Host of Potato leafroll virus

Plant Disease ◽  
2002 ◽  
Vol 86 (5) ◽  
pp. 559-559 ◽  
Author(s):  
P. E. Thomas
Keyword(s):  
Green Peach Aphid ◽  
Potato Crop ◽  
Potato Leafroll Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Columbia Basin ◽  
First Report ◽  
Narrow Host Range ◽  
Potato Plants ◽  
Solanum Sarrachoides ◽  
Leafroll Virus

Potato leafroll virus (PLRV) (genus Polerovirus, family Luteoviridae) is of great economic importance. It has a very narrow host range consisting of only 21 known species, 14 of which are Solanaceae. Ten aphid species transmit PLRV in a persistent manner, but the green peach aphid (Myzus persicae Sulzer) is by far the most important vector and the only important vector in the Columbia Basin of the Pacific Northwest (3). Solanum sarrachoides (Sendtner) may be especially important in the epidemiology of PLRV because it is a predominant weed in potatoes (S. tuberosum L.), other annual crops, and waste areas of the Columbia Basin (1), and herbicides available for potato are relatively ineffective against this weed (2). Since our research began in 1974, we have routinely observed many S. sarrachoides plants throughout the Columbia Basin that acquired an abnormal erect stiff appearance in middle to late summer. Their leaflets curled upward at the margins and expressed interveinal chlorosis and necrosis and a reddened or purple color on abaxial surfaces. The presence of PLRV in symptomatic plants was routinely detected by double-antibody sandwich enzyme-linked immunosorbent assay and by density gradient sedimentation and light absorbance properties of the purified virus when we evaluated the host as a source of purified PLRV in 1980. A systematic study to satisfy the requirements of Koch's postulates to prove that PLRV causes the disease in S. sarrachoides was conducted in 1998. The virus produced typical PLRV symptoms on three young Physalis floridana, Datura stramonium, and potato plants when transmitted by the green peach aphid from each of four symptomatic S. sarrachoides collected from widely spaced potato fields in the Columbia Basin and from purified virus preparations from these plants. Back-transmission from each of these hosts and from purified preparations to S. sarrachoides plants observed the same symptoms. The green peach aphid readily colonized S. sarrachoides in the field, and populations on this host were typically higher than on adjacent potato plants in potato fields. The concentration of PLRV was approximately equal in infected S. sarrachoides and P. floridana plants, as was the efficiency of virus transmission from these hosts by individual green peach aphids. The aphids used in transmission efficacy assays were all the same clone and were reared on the host species assayed. The incidence of PLRV infection among S. sarrachoides in potato fields was typically higher than in the potato crop. Only two other summer annual hosts of PLRV, Amaranthus caudatus and S. nigrum, occur sporadically in potato fields of the Columbia Basin (1). The virus rarely infected these species. To our knowledge, this is the first report that S. sarrachoides is highly susceptible to PLRV and may play an important role in PLRV epidemiology in the Columbia Basin. References: (1) A. G. Ogg, Jr. and B. S. Rogers. Rev. Weed Sci. 4:25, 1989. (2) L. S. Quakenbush and R. N. Anderson. Weed Sci. 33:386, 1985. (3) P. E. Thomas et al. Plant Dis. 81:1311, 1997.

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