Sense and Antisense RNA-Mediated Resistance to Potato Leafroll Virus in Russet Burbank Potato Plants

1991 ◽  
Vol 4 (3) ◽  
pp. 247 ◽  
Author(s):  
L. M. Kawchuk
Keyword(s):  
Antisense Rna ◽  
Russet Burbank Potato ◽  
Potato Leafroll Virus ◽  
Russet Burbank ◽  
Potato Plants ◽  
Leafroll Virus

scholarly journals Reduced Field Spread of Potato Leafroll Virus in Potatoes Transformed with the Potato Leafroll Virus Coat Protein Gene

Plant Disease ◽  
1997 ◽  
Vol 81 (12) ◽  
pp. 1447-1453 ◽  
Author(s):  
P. E. Thomas ◽  
W. K. Kaniewski ◽  
E. C. Lawson
Keyword(s):  
Coat Protein ◽  
Field Test ◽  
Russet Burbank Potato ◽  
Infected Plant ◽  
Virus Titer ◽  
Potato Leafroll Virus ◽  
Russet Burbank ◽  
Expression Vectors ◽  
Cp Gene ◽  
Leafroll Virus

Russet Burbank potato was transformed with plant expression vectors containing the potato leafroll luteovirus (PLRV) coat protein (CP) gene. Transgenic potato lines contained a gene expression cassette with two copies of a PLRV CP gene in which the nucleotide sequence was modified to improve expression of the gene. In addition, the two copies of the PLRV CP gene were each driven by a different promoter. Field test screening for PLRV resistance identified 15 lines which showed moderate resistance to PLRV infection and virus titer build-up and a longer incubation period for systemic infection. By conducting field resistance assays during a period when the vector of PLRV was not present, it was possible to test whether the observed resistance was sufficient to restrict aphid transmission of PLRV in a field test. Two years of field testing demonstrated that PLRV-spread from an infected plant to adjacent healthy plants of the same line was severely restricted in nearly all the transgenic lines in the field. These lines have useful resistance to PLRV and could aid in managing PLRV disease in Russet Burbank potato.

Infection of potato plants with potato leafroll virus changes attraction and feeding behaviour of Myzus persicae

2007 ◽  
Vol 125 (2) ◽  
pp. 135-144 ◽  
Author(s):  
A. E. Alvarez ◽  
E. Garzo ◽  
M. Verbeek ◽  
B. Vosman ◽  
M. Dicke ◽  
...  
Keyword(s):  
Myzus Persicae ◽  
Feeding Behaviour ◽  
Potato Leafroll Virus ◽  
Potato Plants ◽  
Leafroll Virus

scholarly journals Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Priyanka Kumari ◽  
Jitesh Kumar ◽  
Ravi Ranjan Kumar ◽  
Mohammad Ansar ◽  
Kumari Rajani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Movement Protein ◽  
Control Plant ◽  
Plant Viruses ◽  
Potato Leafroll Virus ◽  
Plant Diseases ◽  
World Population ◽  
Genome Replication ◽  
Potato Plants ◽  
Leafroll Virus

AbstractViruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of virion particles is completed inside the host plant, mature virions or sometimes naked viral genomes spread cell-to-cell through plasmodesmata by interacting with the virus-encoded movement protein (MP). We used the RNAi approach to suppress MP gene expression, which in turn prevented potato leafroll virus (PLRV) systemic infection in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with MP siRNA constructs exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of MP gene expression is an efficient method for generating PLRV-resistant potato plants. Further, we identified novel ATPase motifs in MP that may be involved in DNA binding and translocation through plasmodesmata. We also showed that the ATPase activity of MP was stimulated in the presence of DNA/RNA. Overall, our findings provide a robust technology to generate PLRV-resistant potato plants, which can be extended to other species. Moreover, this approach also contributes to the study of genome translocation mechanisms of plant viruses.

Localization of potato leafroll virus in leaves of secondarily-infected potato plants

1995 ◽  
Vol 101 (5) ◽  
pp. 567-571 ◽  
Author(s):  
J. F. J. M. van den Heuvel ◽  
C. M. de Blank ◽  
D. Peters ◽  
J. W. M. van Lent
Keyword(s):  
Potato Leafroll Virus ◽  
Potato Plants ◽  
Leafroll Virus
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