Evaluation of an automated spectrophotometric assay for the determination of total sialic acid in canine serum

1994 ◽  
Vol 18 (5) ◽  
pp. 337-345 ◽  
Author(s):  
A. V. Thougaard ◽  
A. L. Jensen ◽  
A. Wenck
Keyword(s):  
Sialic Acid ◽  
Spectrophotometric Assay ◽  
Total Sialic Acid ◽  
Canine Serum

Validation of an Automated Spectrophotometric Assay for the Determination of Cholinesterase Activity in Canine Serum

2006 ◽  
Vol 30 (7) ◽  
pp. 723-733 ◽  
Author(s):  
T. Furlanello ◽  
G. Simonato ◽  
M. Caldin ◽  
D. De Lorenzi ◽  
G. Lubas ◽  
...  
Keyword(s):  
Cholinesterase Activity ◽  
Spectrophotometric Assay ◽  
Canine Serum

ACTION OF DIETHYLSTILBOESTROL ON MOUSE VAGINAL SIALIC ACIDS (I)

1969 ◽  
Vol 62 (4) ◽  
pp. 657-662 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Acid Concentration ◽  
Dose Response ◽  
Response Curve ◽  
Sialic Acids ◽  
Mucous Cells ◽  
Total Sialic Acid ◽  
Close Relationship ◽  
Dose Dependent

ABSTRACT Diethylstilboestrol administered in lower doses than necessary to induce full vaginal cornification in spayed mice, resulted in vaginal mucification. The thickness of the layer of mucous cells was found to be dose dependent; it was low in the controls, reached a maximum at a dose of 0.02 μg, but full cornification and absence of mucification was present at 0.05 μg. Sialic acid was present in the mucoproteins, and determination of the total amount of sialic acid per vagina showed a close relationship to the histologically evaluated degree of mucification. The ratio between total sialic acid per vagina and vaginal weight (»sialic acid concentration«) gave a dose response curve with a λ value of −0.061. The possibility of using this parameter for oestrogen assay is discussed.

Evaluation of three assays for the determination of serum total sialic acid

1993 ◽  
Vol 26 (6) ◽  
pp. 449-454 ◽  
Author(s):  
M. Crook ◽  
M. Haq ◽  
P. Tutt
Keyword(s):  
Sialic Acid ◽  
Total Sialic Acid

COMPARATIVE ACTION OF VARIOUS OESTROGENIC COMPOUNDS ON MOUSE VAGINAL SIALIC ACIDS (II)

1969 ◽  
Vol 62 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Response Curve ◽  
Sialic Acids ◽  
Clear Cut ◽  
Suitable Parameter ◽  
Sufficient Degree ◽  
Comparative Action ◽  
Relative Potencies ◽  
Good Agreement

ABSTRACT Oestrogens administered in lower doses than necessary to induce full cornification of the mouse vagina induce mucification. It was shown previously that the degree of mucification could be estimated by quantitative determination of sialic acids. A suitable parameter for oestrogen assay was the measurement of vaginal sialic acid concentration which exhibited a clear cut dose response curve. Eleven assays of various oestrogens were performed with this method. Their estimated relative potencies were in good agreement with other routine oestrogen assays. A statistically sufficient degree of precision was found. The sensitivity was of the same order, or slightly higher, than the Allen-Doisy test.

Salivary Total Sialic Acid Levels Increase in Breast Cancer Patients: A Preliminary Study

2011 ◽  
Vol 7 (5) ◽  
pp. 443-447 ◽  
Author(s):  
Leyla Koc Ozturk ◽  
Ebru Emekli-Alturfan ◽  
Emel Kasikci ◽  
Gokhan Demir ◽  
Aysen Yarat
Keyword(s):  
Breast Cancer ◽  
Sialic Acid ◽  
Cancer Patients ◽  
Breast Cancer Patients ◽  
Total Sialic Acid ◽  
Preliminary Study

Studies on the к-casein complex: II. The isolation of a sialic acid-containing fraction by disrupting the complex at low pH in the presence of sodium chloride

1965 ◽  
Vol 32 (1) ◽  
pp. 57-63 ◽  
Author(s):  
R. Beeby
Keyword(s):  
Sialic Acid ◽  
Sodium Chloride ◽  
Deae Cellulose ◽  
Low Ph ◽  
The Other ◽  
Trichloracetic Acid ◽  
Complex Ii ◽  
Total Sialic Acid

SummaryWhen crude к-casein was precipitated at pH 3 in the presence of 0·4m-NaCl the supernatant contained up to 80% of the total sialic acid but no detectable cystine or cysteine. Two fractions were obtained from this supernatant by chromatography on DEAE cellulose; one containing 4–6% sialic acid and the other only onetenth of this amount.Most of the sialic acid of the sialic acid-rich fraction was soluble in 12% trichloracetic acid following treatment with rennin. It is suggested that the glycopeptide released by the action of the enzyme on casein originates from this fraction.

The evaluation of serum total sialic acid and lipid-bound sialic acid levels in chronically exposed rats to 7,12-dimethylbenz(a)anthracene and fluoride

2017 ◽  
Author(s):  
Gokhan Oto ◽  
Suat Ekin ◽  
Hasan Uyar ◽  
Hulya Ozdemir ◽  
Damla Yıldız ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Total Sialic Acid
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