Rapid and sensitive chromatographic determination of free sialic acid in complex bio-pharma fermentation media samples

2012 ◽  
Vol 4 (9) ◽  
pp. 2668 ◽  
Author(s):  
Hassan Alwael ◽  
Damian Connolly ◽  
Brett Paull
Keyword(s):  
Sialic Acid ◽  
Chromatographic Determination ◽  
Free Sialic Acid ◽  
Fermentation Media

scholarly journals Free and bound forms of sialic acid in blood plasma of patients with chronic lymphocytic leukemia

2015 ◽  
Vol 6 (2) ◽  
pp. 108-112 ◽  
Author(s):  
O. V. Netronina
Keyword(s):  
Sialic Acid ◽  
Chronic Lymphocytic Leukemia ◽  
Blood Plasma ◽  
Sialic Acids ◽  
Lymphocytic Leukemia ◽  
General Level ◽  
Control Group ◽  
Free Sialic Acid ◽  
In Blood Plasma

The level of free and bound forms of sialic acids (SА) was investigated in chronic lymphocytic leukemia (CLL) and at different stages of receiving chemotherapy. Determination of sialic acid in blood plasma of patients with CLL before treatment were carried out on the first day and 2 months after taking chemotherapy drugs for combination schemes. Hematologically healthy donors represented the control group. Determination of SA was conducted by thiobarbituric method using trichloroacetic acid for distribution of total sialic acids onto free, protein bound sialic acid, and oligo bound sialic acid. Levelof free sialic acid in serum in chronic lymphocytic leukemia was equal to 43.2% compared to the total number of plasma. On the first day of chemotherapy on background of the general level of oligo bound sialic acids we observed increase in free sialic acid by 24.2% compared to patients not receiving treatment. The level of sialic acids increased 2.6 times compared to norm and featured no significant changes at different stages of treatment. The concentration of sialic acids bound to proteins at 73.4% was lower compared to the control group. After receiving chemotherapy on the first day there was a decline of this indicator to 56.2% compared to groups of patients before treatment. Two months after the treatment the level of all parameters under study returned back to the values obtained at the start of treatment. This data can serve as an additional efficiency criterion of the chemotherapy.

COMPARATIVE ACTION OF VARIOUS OESTROGENIC COMPOUNDS ON MOUSE VAGINAL SIALIC ACIDS (II)

1969 ◽  
Vol 62 (4) ◽  
pp. 663-670 ◽  
Author(s):  
Lars Carlborg
Keyword(s):  
Sialic Acid ◽  
Response Curve ◽  
Sialic Acids ◽  
Clear Cut ◽  
Suitable Parameter ◽  
Sufficient Degree ◽  
Comparative Action ◽  
Relative Potencies ◽  
Good Agreement

ABSTRACT Oestrogens administered in lower doses than necessary to induce full cornification of the mouse vagina induce mucification. It was shown previously that the degree of mucification could be estimated by quantitative determination of sialic acids. A suitable parameter for oestrogen assay was the measurement of vaginal sialic acid concentration which exhibited a clear cut dose response curve. Eleven assays of various oestrogens were performed with this method. Their estimated relative potencies were in good agreement with other routine oestrogen assays. A statistically sufficient degree of precision was found. The sensitivity was of the same order, or slightly higher, than the Allen-Doisy test.

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