Application of the direct beta counter Matrix 96 for cytotoxic assays: Simultaneous processing and reading of 96 wells using a51Cr-retention assay

1993 ◽  
Vol 36 (6) ◽  
pp. 351-356 ◽  
Author(s):  
Gilda G. Hillman ◽  
Norbert Roessler ◽  
Richard S. Fulbright ◽  
J. Edson Pontes ◽  
Gabriel P. Haas
Keyword(s):  
Simultaneous Processing ◽  
Beta Counter

scholarly journals A common protocol for the simultaneous processing of multiple clinically relevant bacterial species for whole genome sequencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kathy E. Raven ◽  
Sophia T. Girgis ◽  
Asha Akram ◽  
Beth Blane ◽  
Danielle Leek ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Dna Extraction ◽  
Genome Sequencing ◽  
Clinical Microbiology ◽  
Bacterial Species ◽  
Outbreak Detection ◽  
Whole Genome ◽  
Library Preparation ◽  
Simultaneous Processing ◽  
Antimicrobial Resistance Gene

AbstractWhole-genome sequencing is likely to become increasingly used by local clinical microbiology laboratories, where sequencing volume is low compared with national reference laboratories. Here, we describe a universal protocol for simultaneous DNA extraction and sequencing of numerous different bacterial species, allowing mixed species sequence runs to meet variable laboratory demand. We assembled test panels representing 20 clinically relevant bacterial species. The DNA extraction process used the QIAamp mini DNA kit, to which different combinations of reagents were added. Thereafter, a common protocol was used for library preparation and sequencing. The addition of lysostaphin, lysozyme or buffer ATL (a tissue lysis buffer) alone did not produce sufficient DNA for library preparation across the species tested. By contrast, lysozyme plus lysostaphin produced sufficient DNA across all 20 species. DNA from 15 of 20 species could be extracted from a 24-h culture plate, while the remainder required 48–72 h. The process demonstrated 100% reproducibility. Sequencing of the resulting DNA was used to recapitulate previous findings for species, outbreak detection, antimicrobial resistance gene detection and capsular type. This single protocol for simultaneous processing and sequencing of multiple bacterial species supports low volume and rapid turnaround time by local clinical microbiology laboratories.

Room Temperature Screw Form Rolling of AZ91D Magnesium Alloy through Processing by Extrusion-Torsion Simultaneous Working

2014 ◽  
Vol 783-786 ◽  
pp. 375-379
Author(s):  
Mitsuaki Furui ◽  
Shouyou Sakashita ◽  
Kazuya Shimojima ◽  
Tetsuo Aida ◽  
Kiyoshi Terayama ◽  
...  
Keyword(s):  
Magnesium Alloy ◽  
Room Temperature ◽  
Hardness Measurement ◽  
Rolling Process ◽  
X Ray Diffraction ◽  
X Ray ◽  
Az91d Magnesium Alloy ◽  
Microstructure Observation ◽  
Fine Grain ◽  
Simultaneous Processing

Extrusion-torsion simultaneous processing is a very attractive technique for fabricating a rod-shape material with fine grain and random texture. We have proposed a new screw form rolling process combined with preliminary extrusion-torsion simultaneous working. Microstructure evolution and mechanical property change of AZ91D magnesium alloy during extrusion-torsion simultaneous processing was examined through microstructure observation, X-ray diffraction analysis and micro-Vickers hardness measurement. By the addition of torsion, the crystal orientation of AZ91D magnesium alloy workpiece was drastically changed from basal crystalline orientation to the random orientation. Crystal grain occurred through the dynamic recrystallization and tended to coarsen with an increase of extrusion-torsion temperature. Grain refinement under 2 um was achieved at the lowest extrusion-torsion temperature of 523 K. M8 gauge AZ91D magnesium alloy screw was successfully formed at room temperature using the extrusion-twisted workpiece preliminary solution treating at 678 K for 345.6 ks. It was found that the extrusion-torsion temperature of 678 K must be selected to fabricate the good screw without any defects.

K‐ABC sequential‐simultaneous processing and language‐impaired preschoolers

1991 ◽  
Vol 7 (4) ◽  
pp. 523-535 ◽  
Author(s):  
Philip W. Ricciardi ◽  
Sylvia Voelker ◽  
Ruth Anne Carter ◽  
Douglas L. Shore
Keyword(s):  
Language Impaired ◽  
Simultaneous Processing

Photonic logic gates with simultaneous processing of multiple signals

2012 ◽  
Author(s):  
Vasile Degeratu ◽  
Ştefania Degeratu ◽  
Paul Şchiopu
Keyword(s):  
Logic Gates ◽  
Multiple Signals ◽  
Simultaneous Processing

Comparisons among Neo-Lurian Assessment Measures of Brain-Injured Adults

1994 ◽  
Vol 74 (3) ◽  
pp. 975-978
Author(s):  
Christopher A. Sink ◽  
Douglas E. Harrington
Keyword(s):  
College Students ◽  
Community College ◽  
Community College Students ◽  
Cognitive Assessment ◽  
Brain Injuries ◽  
Neuropsychological Battery ◽  
Simultaneous Processing ◽  
Brain Injured ◽  
Assessment Measures ◽  
Pearson Correlations

The performances of 49 brain-injured community college students (41% women; M age = 34.0 yr., SD = 13.6) on two neo-Lurian assessment batteries were investigated. Pearson correlations among the 11 clinical subtests of the Luria-Nebraska Neuropsychological Battery, Form I and 10 Planning, Attention, Simultaneous Processing, and Successive Processing (PASS) experimental tasks are reported. While the correlations were largely weak to moderate, a few interpretable trends in these relationships emerged. Over-all, the irregular and diffuse pattern of significant correlations may, in part, reflect the heterogeneity of the Luria-Nebraska battery's subscales. Implications for the cognitive assessment and remediation of patients with brain injuries are briefly discussed.

Multi tissue processing for single cell sequencing of human immune cells v1

2021 ◽  
Author(s):  
Daniel Rainbow ◽  
Sarah Howlett ◽  
Lorna Jarvis ◽  
Joanne Jones
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Immune Cells ◽  
Human Tissues ◽  
Single Cell Sequencing ◽  
Tissue Processing ◽  
Simultaneous Processing ◽  
Single Cell Rna Sequencing ◽  
Human Immune

This protocol has been developed for the simultaneous processing of multiple human tissues to extract immune cells for single cell RNA sequencing using the 10X platform, and ideal for atlasing projects. Included in this protocol are the steps needed to go from tissue to loading the 10X Chromium for single cell RNA sequencing and includes the hashtag and CiteSeq labelling of cells as well as the details needed to stimulate cells with PMA+I.

K–ABC Simultaneous Processing, Das Nonverbal Reasoning, and Horn's Expanded Fluid-Crystallized Theory

1999 ◽  
Vol 84 (2) ◽  
pp. 563-574 ◽  
Author(s):  
David V. Daleo ◽  
Brian R. Lopez ◽  
Jason C. Cole ◽  
Alan S. Kaufman ◽  
Nadeen L. Kaufman ◽  
...  
Keyword(s):  
Factor Analysis ◽  
Fluid Intelligence ◽  
Principal Factor ◽  
Considerable Extent ◽  
Differential Ability Scales ◽  
Assessment Battery ◽  
Clear Cut ◽  
Simultaneous Processing ◽  
Differential Ability ◽  
Nonverbal Reasoning

Horn's distinction between fluid intelligence (Gf) and visualization (Gv) was investigated with two Nonverbal Reasoning subtests from the Differential Ability Scales and three Simultaneous Processing subtests from the Kaufman Assessment Battery for Children. The sample comprised a predominantly Euro-American group of 57 normal boys and girls between the ages of 6 and 12 years. Principal factor analysis yielded clear-cut Gf and Gv dimensions. The Gf factor was composed both of Differential Ability Scales and Kaufman–ABC subtests, suggesting that the construct of simultaneous processing is not merely a measure of Gv, as some researchers have hypothesized, but also measures Horn's Gf fluid intelligence to a considerable extent.

scholarly journals Do nonword repetition errors in children with specific language impairment reflect a weakness in an unidentified skill specific to nonword repetition or a deficit in simultaneous processing?

2006 ◽  
Vol 27 (4) ◽  
pp. 569-573 ◽  
Author(s):  
Klara Marton
Keyword(s):  
Language Impairment ◽  
Specific Language Impairment ◽  
Cognitive Abilities ◽  
Alternative Hypothesis ◽  
Nonword Repetition ◽  
Specific Language ◽  
Speech Stimuli ◽  
Simultaneous Processing ◽  
Error Analyses ◽  
Double Deficit

This Commentary supports Gathercole's (2006) proposal on a double deficit in children with specific language impairment (SLI). The author suggests that these children have a limited phonological storage combined with a particular problem of processing novel speech stimuli. According to Gathercole, there are three areas of skill contributing to memory for nonwords: general cognitive abilities, phonological storage, and an unidentified skill specific to nonword repetition. The focus of this Commentary is to examine whether these children's nonword repetition performance is influenced by an unidentified skill or some other processes. An alternative hypothesis is that the nonword repetition errors observed in children with SLI are related to one of their main weaknesses, to their difficulties in simultaneous processing of information. Evidence for this argument comes from our recent studies: from error analyses data and from findings on nonword repetition with stimuli that included meaningful parts (monosyllabic real words).

scholarly journals Laboratory Organization

1993 ◽  
Keyword(s):  
Tissue Culture ◽  
Dna Analysis ◽  
Simple Process ◽  
Cold Room ◽  
Chain Of Custody ◽  
Trade Shows ◽  
New Facility ◽  
Beta Counter ◽  
Dark Room

Laboratory organization involves both the physical establishment and its operation. It is perhaps simplest to divide the laboratory into its component sections and discuss each separately. The areas may physically overlap for a small facility, and depending on the operation specialty, some sections, such as tissue culture or probe amplification, may not be required. Unless the operation is large, dark room and cold room facilities and expensive equipment such as an ultracentrifuge and beta counter are best shared, if feasible. The setup of a DNA analysis facility is a relatively simple process if it is incorporated into an established biochemistry program; it is considerably more involved if no such base exists. The outline presented in this chapter is only a guide; individuals contemplating the development of a new facility should visit as many established centers as possible. Discussions with sales representatives and attendance at relevant trade shows and DNA conferences are invaluable. Office requirements for a DNA program are no different in principle from those of any other biochemistry program. At least one separate office is required, usually for the program director and, as space permits, offices for a clerk-secretary and senior technologist are useful. Everyone working in the laboratory must have at least a small partitioned desk space in a quiet location. Lockable fire-resistant cabinets are required to store sensitive records; these cabinets should be accessible, preferably located in the clerical area. Analysis results are worthless without proper documentation of a specimen’s chain of custody (continuity). Information, including time and conditions of specimen procurement, conditions of storage and shipment, date received by the laboratory, and reason for the analysis request is also required. These data can be manually recorded; however, entry into a computer program capable of sorting and maintaining records for long-term retrieval is almost mandatory. Storage of unprocessed specimens may be necessary, and if at all possible, DNA should be isolated when received.

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