Survey of sensitivity of 22 strains of yeasts to T-2 toxin in relation to growth on glucose and glycerol medium

James A. Baxter; Loo Sar Chia; Dick W. Hsieh; Sushil K. Datta

doi:10.1007/bf01691794

One-Pot Three Component Synthesis of 2-(1H-Benzo[d]thiazole-2-yl)- N-Arylbenzamides in Glycerol Medium

Asian Journal of Chemistry ◽

10.14233/ajchem.2020.22538 ◽

2020 ◽

Vol 32 (6) ◽

pp. 1343-1351

Author(s):

Swathi Thumula ◽

Venkatesan Srinivasadesikan ◽

Ravi K. Kottalanka ◽

S. Rex Jeya Rajkumar ◽

Balajee Ramchandran

Keyword(s):

Docking Study ◽

Molecular Docking Study ◽

One Pot ◽

Docking Result ◽

Three Component Reaction ◽

Antibacterial Target ◽

Short Time ◽

A549 Lung ◽

Glycerol Medium ◽

Mcf 7

In this work, a series of 2-(1H-benzo[d]thiazole-2-yl)-N-arylbenzamides is synthesized by using diethyl phthalate, anilines and 2-amino-benzenethiol by one-pot three component synthesis in glycerol medium. Phosphoric acid is used as an effective reagent for this one-pot three component reaction. This reaction got completed in a short time, easy workup and gave an excellent yield in glycerol medium. The N-arylbenzamides was found to have significant cytotoxic potentials against various cancer cells viz., A549 (lung cancer), HeLa (cervical cancer) and MCF-7 (breast cancer) using MTT assay. The molecular docking study is also employed to understand the binding mechanism of N-arylbenzamides against the antibacterial target. The docking result shows the binding energy of compound 4a is -8.6 kcal/mol. The binding affinity is a major concern and it shows that Asn and Thr residues have an interaction with compound 4a.

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THIAMINE – INDUCED FORMATION OF THE MONOPYRROLE MOIETY OF PRODIGIOSIN

Baghdad Science Journal ◽

10.21123/bsj.4.4.622-627 ◽

2007 ◽

Vol 4 (4) ◽

pp. 622-627 ◽

Cited By ~ 1

Author(s):

Baghdad Science Journal

Keyword(s):

Serratia Marcescens ◽

Yeast Extract ◽

Type Strain ◽

The Other ◽

Red Pigment ◽

Enhanced Production ◽

Pyrimidine Moiety ◽

Glycerol Medium

Thiamine stimulates the production of a red pigment , which is chromatographically and spectrophotometrically identical to prodigiosin , by growing cultures of serratia marcescens mutant 9-3-3 . this mutant is blocked in the formation of 2- methyl -3- amyl pyrorol( MAP),the monopyrrole moiety of prodigiosin , but accumulates 4-methoxy-2, 2-bipyrrole -5- carboxaldehyde (MBC) and can couple this compound with( MAP) to form prodigiosin . Addition of thiamine caused production of( MAP) , and as little as 0.02 mg of thiamine / ml in peptone- glycerol medium stimulated production of measurable amounts of prodigiosin. Phosphate saltes and another type of peptone decreased the thiamine- induced formation of prodigiosin ,yeast extract and glycerol enhanced formation of this substance. Thiamine also enhanced production of prodigiosin by wiled – type Strain Nima of S. marcescens . The pyrimidine moiety of thiamine was also 10% as effective as the vitamin ; the thiazol moiety only 4% , and the two moieties together , 25% . Thiamine did not stimulate production of prodigiosin biosynthesis as strain 9-3-3 . This is not surprising since strain 9-3-3 originated as a result of two mutational events one event may involve thiamine directly , and the other may involve the biosynthesis of( MAP).

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An experimental study of the dynamics of ascent of the bubble system in the presence of a surfactant

MATEC Web of Conferences ◽

10.1051/matecconf/201819401002 ◽

2018 ◽

Vol 194 ◽

pp. 01002

Author(s):

Alexandra Antonnikova ◽

Sergey Basalaev ◽

Anna Usanina ◽

Eugene Maslov

Keyword(s):

Experimental Study ◽

Reynolds Numbers ◽

Experimental Setup ◽

Bubble Cluster ◽

Bubble System ◽

Wide Range ◽

Compact Cluster ◽

Glycerol Medium

This paper presents investigations on the new experimental setup for obtaining a compact cluster of monodisperse bubbles of a given diameter is presented. Also we provided the results of experimental study of the bubble cluster floating-up in the presence of a surfactant in a wide range of Reynolds numbers. There was held a comparison of the dynamics of the floating-up of a monodisperse bubble cluster in a glycerol medium and in the medium glycerin supplemented with a surfactant.

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THE EFFECT OF GLYCEROL MEDIUM ON THE ACTIVATION OF HUMAN PLASMINOGEN BY STREPTOKINASE AND ON THE ACTION OF THE ENZYME, PLASMIN

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y62-135 ◽

1962 ◽

Vol 40 (1) ◽

pp. 1203-1212 ◽

Cited By ~ 1

Author(s):

Phyllis S. Roberts

Keyword(s):

Methyl Ester ◽

Phosphate Buffer ◽

Water Medium ◽

Rate Of Hydrolysis ◽

Human Plasminogen ◽

Lag Period ◽

Hydrolysis Of ◽

Glycerol Medium

A 50% glycerol medium affected the activation of plasminogen by streptokinase (SK) and the activity of plasmin (formed by glycerol incubation) on p-toluene-sulphonyl-L-arginine methyl ester (TAMe) in the following ways:(1) TAMe interfered strongly with the activation of plasminogen by SK in a glycerol medium but not in a water medium under the same conditions.(2) SK inhibited the action of plasmin on TAMe in a glycerol medium, but in a water medium SK increased the rate of hydrolysis of TAMe, the extent of the increase depending upon pH.(3) Phosphate buffer inhibited the activation of plasminogen by SK in a glycerol medium but not in a water medium. In the glycerol medium a lag period in the formation of enzyme was found, particularly noted with a phosphate buffer, and the lag period increased with increasing concentration of the buffer.(4) Phosphate buffer inhibited the action of plasmin on TAMe in a glycerol medium but not in a water medium.Mechanisms of activation of plasminogen by SK are discussed. The data presented support the postulate that a reaction takes place between plasmin and SK to form an enzyme called "activator".

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THE EFFECT OF GLYCEROL MEDIUM ON THE ACTIVATION OF HUMAN PLASMINOGEN BY STREPTOKINASE AND ON THE ACTION OF THE ENZYME, PLASMIN

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o62-135 ◽

1962 ◽

Vol 40 (9) ◽

pp. 1203-1212 ◽

Cited By ~ 8

Author(s):

Phyllis S. Roberts

Keyword(s):

Methyl Ester ◽

Phosphate Buffer ◽

Water Medium ◽

Rate Of Hydrolysis ◽

Human Plasminogen ◽

Lag Period ◽

Hydrolysis Of ◽

Glycerol Medium

A 50% glycerol medium affected the activation of plasminogen by streptokinase (SK) and the activity of plasmin (formed by glycerol incubation) on p-toluene-sulphonyl-L-arginine methyl ester (TAMe) in the following ways:(1) TAMe interfered strongly with the activation of plasminogen by SK in a glycerol medium but not in a water medium under the same conditions.(2) SK inhibited the action of plasmin on TAMe in a glycerol medium, but in a water medium SK increased the rate of hydrolysis of TAMe, the extent of the increase depending upon pH.(3) Phosphate buffer inhibited the activation of plasminogen by SK in a glycerol medium but not in a water medium. In the glycerol medium a lag period in the formation of enzyme was found, particularly noted with a phosphate buffer, and the lag period increased with increasing concentration of the buffer.(4) Phosphate buffer inhibited the action of plasmin on TAMe in a glycerol medium but not in a water medium.Mechanisms of activation of plasminogen by SK are discussed. The data presented support the postulate that a reaction takes place between plasmin and SK to form an enzyme called "activator".

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