The effects of vital dyes on living organisms with special reference to Methylene Blue and Neutral Red

1971 ◽  
Vol 3 (1) ◽  
pp. 71-93 ◽  
Author(s):  
Pedro Barbosa ◽  
T. Michael Peters
Keyword(s):  
Methylene Blue ◽  
Special Reference ◽  
Neutral Red ◽  
Vital Dyes ◽  
Living Organisms

A Study of the Spheroid System of Sympathetic Neurones with Special Reference to the Problem of Neurosecretion

1948 ◽  
Vol s3-89 (7) ◽  
pp. 333-350
Author(s):  
OWEN LEWIS THOMAS
Keyword(s):  
Special Reference ◽  
Neutral Red ◽  
Golgi Body ◽  
Living Cells ◽  
Metallic Particles ◽  
Vital Dyes ◽  
Dispersed System ◽  
Sympathetic Neurones

1. Evidence is put forward in support of the view that the Golgi complement of the vertebrate sympathetic neurone consists of a dispersed system of spherical bodies. These bodies can be studied in living cells and with vital dyes and each consists of a neutral red staining core (the vacuome of Parat) enveloped in a lipoidal sheath. 2. The classical Golgi body is shown to be an artifact produced within the cell. With the osmium techniques the spheroid bodies together with the mitochondria form a framework which serves as a centre for a non-specific deposition of metallic particles. 3. The Golgi spheroids exhibit a secretion cycle with the formation of a granular product. The granules are identified with the ‘neurosecretion’ granules of Scharrer. 4. These granules appear to be transported to the nucleus of the cell and there to be absorbed. This observed interrelationship between nucleus and Golgi product is discussed.

A simple technique for the preservation of vital dyes in fixed and sectioned embryos

Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 131-132
Author(s):  
J. Pertusa
Keyword(s):  
Methylene Blue ◽  
Ethyl Alcohol ◽  
Toluidine Blue ◽  
Trypan Blue ◽  
Simple Technique ◽  
Nile Blue ◽  
Neutral Red ◽  
The Other ◽  
Serial Sectioning ◽  
Vital Dyes

In embryological work using vital dyes it is highly desirable to be able to study the distribution of the dyes in fixed material, whether examined in toto or after embedding in paraffin and serial sectioning. However, both fixation and dehydration present problems for the preservation of colour in vitally stained cells. Some fixatives preserve some dyes but, so far as I am aware, none will preserve all the vital dyes in common use. On the other hand, ethyl alcohol destroys or dissolves all vital dyes and its use in dehydration is thus undesirable. Among the fixatives that have been proposed are those of Golowin (1902), Mitamura (1923), Parat & Painlevé (1925), and Tchéou Tai Chuin (1930) for neutral red; that of Izquierdo (1955) for toluidine blue; that of Gérard (1925) for Trypan blue; that of Turchini (1919) for methylene blue; that of Lehmann (1929) for Nile blue.

Chemisorption of methylene blue by kaolinite

Clay Minerals ◽  
1967 ◽  
Vol 7 (1) ◽  
pp. 19-31 ◽  
Author(s):  
F. A. Faruqi ◽  
Susumu Okuda ◽  
W. O. Williamson
Keyword(s):  
Methylene Blue ◽  
Special Reference ◽  
Cation Exchange ◽  
High Ph ◽  
Inorganic Cations ◽  
Ph Values ◽  
Aqueous Suspensions ◽  
Exchange Site

AbstractThe chemisorption of methylene blue by kaolinite crystals increased as the aqueous suspensions changed from acid to alkaline because, at high pH values, not only the basal pinacoids but the edge-faces were negatively charged. The inability to calculate acceptable specific surfaces or cation exchange capacities from the chemisorption data is discussed, with special reference to the orientation of adsorbed dye cations, the covering of more than one exchange site by a monomer or polymer, the different concentrations of exchange sites on the basal pinacoids and edge-faces respectively, the possibility that such sites occur on the tetrahedral rather than on the octahedral basal pinacoid, and the incomplete replacement of inorganic cations.

The effect of physico-chemically immobilized methylene blue and neutral red on the anode of microbial fuel cell

2012 ◽  
Vol 17 (2) ◽  
pp. 361-370 ◽  
Author(s):  
Arseniy L. Popov ◽  
Jung Rae Kim ◽  
Richard M. Dinsdale ◽  
Sandra R. Esteves ◽  
Alan J. Guwy ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Neutral Red

scholarly journals Experiments on the Effect of Dyes on Induction and Respiration in the Amphibian Gastrula

1939 ◽  
Vol 16 (2) ◽  
pp. 150-154
Author(s):  
R. A. BEATTY ◽  
S. DE JONG ◽  
M. A. ZIELIŃSKI
Keyword(s):  
Methylene Blue ◽  
Oxygen Consumption ◽  
Weak Solutions ◽  
Neural Differentiation ◽  
Neutral Red ◽  
Cell Respiration ◽  
Janus Green ◽  
Method Show ◽  
Weak Concentration

1. It is shown that pieces of presumptive epidermis (ventral ectoderm of the gastrula), when isolated into weak solutions of several dyes, will undergo neural differentiation. Dyes such as Janus green and neutral red, which are not known to accelerate cell respiration, appear to have this effect, as well as methylene blue, the accelerating action of which on cell respiration is well known. 2. Measurements of the oxygen consumption of isolated pieces of the gastrula by the Cartesian Diver method show that methylene blue, if in weak concentration, has an accelerating action of about 45%. In stronger concentrations it is inhibitory.

The Effects of Single Doses of Pilocarpine and of Histamine upon the Granules of Oxyntic Cells of Rats, with Special Reference to their Phospholipine Content

1952 ◽  
Vol s3-93 (21) ◽  
pp. 97-104
Author(s):  
GORDON MENZIES
Keyword(s):  
Special Reference ◽  
Phase Contrast ◽  
Neutral Red ◽  
Granule Size ◽  
Complete Loss ◽  
Exact Nature ◽  
Gastric Glands ◽  
Phase Contrast Microscope ◽  
Contrast Microscope

1. New data are presented on the morphology and cytochemistry of the granules of the oxyntic cells of the rat's stomach in continuation of work previously reported (Menzies, 1949). The effects of single injections of pilocarpine and of histamine on the granules were investigated, with particular reference to their phospholipine content as shown by Baker's acid haematein method. 2. Pilocarpine causes a marked enlargement of the granules with loss of phospholipine from the central part of many of the larger granules, and complete loss of phospholipine from others. The granules that have lost their phospholipine probably still contain lipoid as shown by sudan black. Pilocarpine also causes marked aggregation of the granules. 3. Histamine causes a less marked increase in granule size and no observable loss of phospholipine, but some of the granules became elongated, an effect that can be seen with the phase-contrast microscope, and there is a tendency for them to recede to the peripheral parts of the cells associated with an enlargement of the intracellular canals. 4. Both pilocarpine and histamine result in some enlargement of the oxyntic cells, the effect being more marked with pilocarpine. 5. Neither pilocarpine nor histamine alters the size or shape of the non-lipoid moiety of the granules. 6. Supravital colouring with neutral red shows a few droplets in oxyntic cells in the bases of the gastric glands. These enlarge slightly after histamine and greatly after pilocarpine, and they are always spherical. It has not yet been possible to decide as to their exact nature.

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