scholarly journals Experiments on the Effect of Dyes on Induction and Respiration in the Amphibian Gastrula

1939 ◽  
Vol 16 (2) ◽  
pp. 150-154
Author(s):  
R. A. BEATTY ◽  
S. DE JONG ◽  
M. A. ZIELIŃSKI
Keyword(s):  
Methylene Blue ◽  
Oxygen Consumption ◽  
Weak Solutions ◽  
Neural Differentiation ◽  
Neutral Red ◽  
Cell Respiration ◽  
Janus Green ◽  
Method Show ◽  
Weak Concentration

1. It is shown that pieces of presumptive epidermis (ventral ectoderm of the gastrula), when isolated into weak solutions of several dyes, will undergo neural differentiation. Dyes such as Janus green and neutral red, which are not known to accelerate cell respiration, appear to have this effect, as well as methylene blue, the accelerating action of which on cell respiration is well known. 2. Measurements of the oxygen consumption of isolated pieces of the gastrula by the Cartesian Diver method show that methylene blue, if in weak concentration, has an accelerating action of about 45%. In stronger concentrations it is inhibitory.

scholarly journals CELL RESPIRATION STUDIES

1927 ◽  
Vol 46 (1) ◽  
pp. 43-51
Author(s):  
Blake C. Wilbur ◽  
Geneva A. Daland ◽  
John Cohen
Keyword(s):  
Oxygen Consumption ◽  
Whole Blood ◽  
Repeated Measurements ◽  
Closed Space ◽  
Cell Respiration ◽  
Chemical Substances ◽  
Normal Individuals ◽  
Tissue Cells

A description is given of a closed space respiration apparatus which can be used to determine the amount of gas used or liberated by living blood or tissue cells, or chemical substances. Continuous observations can be made and repeated measurements recorded without interrupting the vital processes or destroying the cells. Studies of the oxygen consumption by whole blood in normal individuals and in patients with leucocytosis and myelogenous leucemia, as well as by white cells suspended in plasma, will be reported in subsequent papers.

Hemodynamic responses to induced tachycardia in methylene blue-treated dogs

1964 ◽  
Vol 206 (6) ◽  
pp. 1279-1280 ◽  
Author(s):  
Skoda Afonso ◽  
George G. Rowe ◽  
Cesar A. Castillo ◽  
Charles W. Crumpton
Keyword(s):  
Methylene Blue ◽  
Blood Flow ◽  
Oxygen Consumption ◽  
Right Ventricular ◽  
Coronary Circulation ◽  
Coronary Vascular Resistance ◽  
Hemodynamic Effects ◽  
Group I ◽  
Anesthetized Dogs ◽  
Group Ii

The present study relates to metabolic, and systemic and coronary hemodynamic effects of methylene blue in five anesthetized dogs (group I) and of electrically induced tachycardia in ten dogs pretreated with methylene blue (group II). In group I body oxygen consumption increased slightly. In group II coronary blood flow, cardiac metabolic rate for oxygen, and right ventricular work increased significantly while coronary vascular resistance decreased significantly. The observed pattern of changes is not different from that produced by induced tachycardia in untreated dogs. Methylene blue does not alter the usual response of coronary circulation to cardiac acceleration.

The effect of physico-chemically immobilized methylene blue and neutral red on the anode of microbial fuel cell

2012 ◽  
Vol 17 (2) ◽  
pp. 361-370 ◽  
Author(s):  
Arseniy L. Popov ◽  
Jung Rae Kim ◽  
Richard M. Dinsdale ◽  
Sandra R. Esteves ◽  
Alan J. Guwy ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Neutral Red

Memory facilitation by methylene blue: Dose-dependent effect on behavior and brain oxygen consumption

2005 ◽  
Vol 511 (2-3) ◽  
pp. 151-158 ◽  
Author(s):  
Penny D. Riha ◽  
Aleksandra K. Bruchey ◽  
David J. Echevarria ◽  
F. Gonzalez-Lima
Keyword(s):  
Methylene Blue ◽  
Oxygen Consumption ◽  
Dependent Effect ◽  
Brain Oxygen ◽  
Memory Facilitation ◽  
Dose Dependent ◽  
Dose Dependent Effect

A simple technique for the preservation of vital dyes in fixed and sectioned embryos

Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 131-132
Author(s):  
J. Pertusa
Keyword(s):  
Methylene Blue ◽  
Ethyl Alcohol ◽  
Toluidine Blue ◽  
Trypan Blue ◽  
Simple Technique ◽  
Nile Blue ◽  
Neutral Red ◽  
The Other ◽  
Serial Sectioning ◽  
Vital Dyes

In embryological work using vital dyes it is highly desirable to be able to study the distribution of the dyes in fixed material, whether examined in toto or after embedding in paraffin and serial sectioning. However, both fixation and dehydration present problems for the preservation of colour in vitally stained cells. Some fixatives preserve some dyes but, so far as I am aware, none will preserve all the vital dyes in common use. On the other hand, ethyl alcohol destroys or dissolves all vital dyes and its use in dehydration is thus undesirable. Among the fixatives that have been proposed are those of Golowin (1902), Mitamura (1923), Parat & Painlevé (1925), and Tchéou Tai Chuin (1930) for neutral red; that of Izquierdo (1955) for toluidine blue; that of Gérard (1925) for Trypan blue; that of Turchini (1919) for methylene blue; that of Lehmann (1929) for Nile blue.

scholarly journals Continuous exposure to high concentrations of nitric oxide leads to persistent inhibition of oxygen consumption by J774 cells as well as extraction of oxygen by the extracellular medium

2000 ◽  
Vol 346 (2) ◽  
pp. 407-412 ◽  
Author(s):  
Antonia ORSI ◽  
Belén BELTRÁN ◽  
Emilio CLEMENTI ◽  
Katarina HALLÉN ◽  
Martin FEELISCH ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oxygen Consumption ◽  
Chemical Interaction ◽  
Continuous Exposure ◽  
Cell Respiration ◽  
Extracellular Medium ◽  
Pathological Conditions ◽  
J774 Cells ◽  
High Concentrations ◽  
Complex I Activity

Nitric oxide (NO) plays a key role in many physiological and pathophysiological events, including the control of cell respiration. Both reversible and irreversible inhibition of mitochondrial respiration have been reported following the generation of NO by cells. We have exposed the murine macrophage cell line J774 to high concentrations of NO, such as are generated in some pathological conditions, and determined their effect on oxygen consumption. We observed a persistent inhibition of respiration which was due to a redox-dependent, progressive inhibition of complex I activity. No other enzyme of the respiratory chain was inhibited in this way. At the same time, we detected a paradoxical removal of oxygen by the extracellular medium. This removal was due to a chemical interaction between dissolved oxygen and NO-related species released from cells exposed to NO. A similar removal of oxygen by the cell supernatant also occurred following activation of cells with cytokines and bacterial products. Thus, the amounts of NO generated during pathological conditions may contribute to tissue hypoxia both by inhibiting cell respiration and by promoting removal of oxygen from the extracellular medium.

Sign in / Sign up

Export Citation Format

Share Document