Species-specific antigens ofMycoplasma hyopneumoniae and cross-reactions with other porcine mycoplasmas

1987 ◽  
Vol 15 (4) ◽  
pp. 233-239 ◽  
Author(s):  
Göran Bölske ◽  
Marie-Louise Strandberg ◽  
Katrin Bergström ◽  
Karl-Erik Johansson
Parasitology ◽  
2017 ◽  
Vol 144 (7) ◽  
pp. 851-868 ◽  
Author(s):  
LUÍS F. P. GONDIM ◽  
JOSÉ R. MINEO ◽  
GEREON SCHARES

SUMMARYToxoplasma gondii, Neosporaspp.,Sarcocystisspp.,Hammondiaspp. andBesnoitia besnoitiare genetically related cyst-forming coccidia. Serology is frequently used for the identification ofT. gondii, Neosporaspp. andB. besnoiti-exposed individuals. Serologic cross-reactions occur in different tests among animals infected withT. gondiiandH. hammondi,as well as among animals infected byT. gondiiandN. caninum. Infections caused byN. caninumandN. hughesiare almost indistinguishable by serology.Neospora caninum, B. besnoitiandSarcocystisspp. infections in cattle show some degree of serologic cross-reactivity. Antibody cross-reactivity betweenNeosporaspp. andH. heydorni-infected animals is suspected, but not proven to occur. We review serologic cross-reactivity among animals and/or humans infected withT. gondii, Neosporaspp.,Sarcocystisspp.,Hammondiaspp. andB. besnoiti. Emphasis is laid upon antigens and serological methods forN. caninumdiagnosis which were tested for cross-reactivity with related protozoa. Species-specific antigens, as well as stage-specific proteins have been identified in some of these parasites and have promising use for diagnosis and epidemiological surveys.


1938 ◽  
Vol 68 (3) ◽  
pp. 335-352 ◽  
Author(s):  
Werner Henle ◽  
Gertrude Henle ◽  
Leslie A. Chambers

1. A method has been described for separation of heads and tails of mammalian spermatozoa. 2. By means of absorption technique applied to homologous spermatozoal sera, head-specific and tail-specific antigens could be demonstrated. Both are heat-labile. 3. A heat-stable antigen was found to be common to both heads and tails. This substance is species-specific. 4. Antibodies against the head- and tail-specific antigens led to two different types of agglutination as shown by the slide method. 5. Using heterologous antisera against spermatozoa three different cross-reacting antigens could be observed, two in the heads, one in the tails. 6. One of the head-antigens is not active in the native cell; it comes to action only after breaking the cell. Antibodies against this substance were not found in antisera against native bull spermatozoa but were formed when vibrated spermatozoa or heads were injected into rabbits. 7. The cross-reactions can be removed from an antiserum leaving the head- as well as the tail-specific reaction intact.


1960 ◽  
Vol 6 (6) ◽  
pp. 661-672
Author(s):  
Anne M. Collins ◽  
I. B. R. Duncan ◽  
Elisabeth M. Neelin ◽  
R. Znamirowski ◽  
T. E. Roy

Polysaccharide-type extracts prepared from a coagulase-positive staphylococcus, a coagulase-negative staphylococcus, and a type 12 hemolytic streptococcus were investigated by means of the hemagglutination technique to determine whether the extract of Staphylococcus pyogenes contained antigens that could detect species-specific antibodies in antisera. The sera used were prepared by inoculating rabbits with vaccines, extract-sensitized erythrocytes, or extract alone. The extracts themselves elicited poor antibody formation unless adsorbed on erythrocytes. Marked serological cross reactions were found among the preparations from all three organisms, and mirror absorption tests failed to yield clear evidence of species-specific factors in the extracts or in the antisera. The latex fixation technique demonstrated similar cross reactions to those found by hemagglutination. The common antigen or complex of antigens found in the extracts of all three organisms were not identified as the non-specific antigens described by Rantz and others. Chemical analyses of the extracts showed that, as prepared, they were complex mixtures of substances to which the term "polysaccharide" could not properly be applied.


1983 ◽  
Vol 258 (17) ◽  
pp. 10481-10487 ◽  
Author(s):  
S W Hunter ◽  
R C Murphy ◽  
K Clay ◽  
M B Goren ◽  
P J Brennan

1960 ◽  
Vol 6 (4) ◽  
pp. 453-462 ◽  
Author(s):  
N. A. Labzoffsky ◽  
A. E. Kelen

Methods of preparation of "whole" and type-specific antigens from leptospiral cultures for use in the complement fixation test are outlined. Leptospiral cultures grown in Korthof's medium were treated with pyridine and after appearance of a copious precipitate were centrifuged. The supernatants were dialyzed and after concentration by pervaporation were used as "whole" antigens. Type-specific antigens were prepared from acetone precipitates of filtrates of "whole" antigens. Considerable cross reaction was observed with "whole" antigens prepared from L. pomona, L. canicola, and L. icterohaemorrhagiae and their respective rabbit hyperimmune sera, although titers with homologous sera were invariably higher. These cross reactions, however, were not as pronounced with sera from cattle naturally infected with L. pomona, where approximately 43% of the sera reacted with homologous antigen only. No reactions were observed between the type-specific antigen and any of the heterologous sera employed. It has also been shown that with experimental sera type-specific antibodies appeared earlier and persisted longer than group-specific antibodies. In view of the above observations the use of polyvalent antigen for routine screening of leptospiral antisera is advocated. Type-specific antigens are recommended for use in more precise diagnosis.


1940 ◽  
Vol 71 (4) ◽  
pp. 539-550 ◽  
Author(s):  
Rebecca C. Lancefield

In any one strain the occurrence of the previously recognized type-specific protein, M, is usually completely correlated with the presence of the recently recognized type-specific antigen, T. Strain C203 is exceptional in having the T substance of type 1 as well as the two type-specific antigens, M and T, characteristic of type 3. It does not have the M antigen of type 1. While other strains with similar antigenic peculiarities have not been encountered, it is probable that they occur, and the existence of such anomalies must be suspected when unusual serological reactions occur.


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