Steady-state and nuclear run-on analyses of transcription in a temperature-sensitive Chinese hamster cell mutant with a defect in RNA metabolism

1988 ◽  
Vol 14 (5) ◽  
pp. 439-459 ◽  
Author(s):  
Thelma C. Slezynger ◽  
Immo E. Scheffler
Keyword(s):  
Steady State ◽  
Chinese Hamster ◽  
Rna Metabolism ◽  
Chinese Hamster Cell ◽  
Temperature Sensitive ◽  
Cell Mutant

Cloning of a human S-phase cell cycle gene: use of transient expression for screening

1987 ◽  
Vol 7 (2) ◽  
pp. 775-779
Author(s):  
A Fainsod ◽  
G Diamond ◽  
M Marcus ◽  
F H Ruddle
Keyword(s):  
Cell Cycle ◽  
Genomic Library ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
S Phase ◽  
Phase Cell ◽  
Cell Cycle Gene ◽  
Temperature Sensitive ◽  
Specific Cell ◽  
Restrictive Temperature

We report here the cloning of a human cell cycle gene capable of complementing a temperature-sensitive (ts) S-phase cell cycle mutation in a Chinese hamster cell line. Cloning was performed as follows. A human genomic library in phage lambda containing 600,000 phages was screened with labeled cDNA synthesized from an mRNA fraction enriched for the specific cell cycle gene message. Plaques containing DNA inserts which hybridized to the cDNA were picked, and their DNAs were assayed for transient complementation in DNA transformation experiments. The transient complementation assay we developed is suitable for most cell cycle genes and indeed for many genes whose products are required for cell proliferation. Of 845 phages screened, 1 contained an insert active in transient complementation of the ts cell cycle mutation. Introduction of this phage into the ts cell cycle mutant also gave rise to stable transformants which grew normally at the restrictive temperature for the ts mutant cells.

Chinese hamster cell mutant, V-C8, a model for analysis of Brca2 function

2006 ◽  
Vol 600 (1-2) ◽  
pp. 79-88 ◽  
Author(s):  
Wouter W. Wiegant ◽  
René M. Overmeer ◽  
Barbara C. Godthelp ◽  
Paul P.W. van Buul ◽  
Małgorzata Z. Zdzienicka
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Cell Mutant

A novel type of X-ray-sensitive Chinese hamster cell mutant with radioresistant DNA synthesis and hampered DNA double-strand break repair

1995 ◽  
Vol 337 (2) ◽  
pp. 119-129 ◽  
Author(s):  
Gerald W.C.T Verhaegh ◽  
Wim Jongmans ◽  
Bruno Morolli ◽  
Nicolaas G.J Jaspers ◽  
Govert P van der Schans ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Chinese Hamster ◽  
Strand Break ◽  
Chinese Hamster Cell ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Cell Mutant ◽  
X Ray ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals Temperature-Sensitive Chinese Hamster Fibroblast Mutant with a Defect in RNA Metabolism

1982 ◽  
Vol 2 (12) ◽  
pp. 1558-1573 ◽  
Author(s):  
Eric A. Wong ◽  
Immo E. Scheffler
Keyword(s):  
Protein Synthesis ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Temperature Sensitive ◽  
Chinese Hamster Fibroblast ◽  
Rna And Protein Synthesis ◽  
Shock Proteins ◽  
Short Time ◽  
Biosynthetic Machinery ◽  
Temperature Sensitive Phenotype

We describe a new temperature-sensitive mutant of Chinese hamster cell fibroblasts. After a shift to the nonpermissive temperature of 40.5°C, the rates of DNA, RNA, and protein synthesis declined rapidly (to ≤50% within 12 h) and the progression of unsynchronized cells through the cell cycle was affected. We believe that DNA synthesis came to a halt after a short time, because cells no longer entered the S phase. The decrease in protein synthesis at 40.5°C was shown to be a consequence of a decrease in the number of polysomes, whereas free 80S ribosomes accumulated. We concluded that the components of the protein biosynthetic machinery were intact (ribosomes and soluble factors), but synthesis was limited by a shortage of mRNA. The decline in mRNA production had a significant effect on the synthesis of proteins (e.g., heat shock proteins) translated from short-lived messages. We observed that both polyadenylated and nonpolyadenylated RNA syntheses declined at 40.5°C, whereas the synthesis of small RNAs (4 to 5S) was less reduced. The argument is made that the temperature-sensitive phenotype is the result of a defect affecting mRNA synthesis.

The Chinese hamster cell mutant V-H4 is homologous to Fanconi anemia (complementation group A)

1991 ◽  
Vol 56 (1) ◽  
pp. 23-26 ◽  
Author(s):  
F. Arwert ◽  
M.A. Rooimans ◽  
A. Westerveld ◽  
J.W.I.M. Simons ◽  
M.Z. Zdzienicka
Keyword(s):  
Fanconi Anemia ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Complementation Group ◽  
Cell Mutant ◽  
Group A
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