scholarly journals Ultrastructural changes during the enhancement of cellular 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase in a Chinese hamster cell mutant resistant to compactin (ML 236B).

1983 ◽  
Vol 8 (3) ◽  
pp. 309-312 ◽  
Author(s):  
Akinori Masuda ◽  
Michihiko Kuwano ◽  
Tatsuo Shimada
Keyword(s):  
Coenzyme A ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Ultrastructural Changes ◽  
Cell Mutant ◽  
Coenzyme A Reductase

scholarly journals Chinese hamster cell mutant resistant to ML236B (Compactin) is defective in endocytosis of low-density lipo-protein.

1982 ◽  
Vol 2 (11) ◽  
pp. 1354-1361 ◽  
Author(s):  
A Masuda ◽  
S Akiyama ◽  
M Kuwano
Keyword(s):  
Coenzyme A ◽  
Spontaneous Mutation ◽  
Low Density Lipoprotein ◽  
Chinese Hamster ◽  
Specific Inhibitor ◽  
Density Lipoprotein ◽  
Chinese Hamster Cell ◽  
Cellular Level ◽  
Low Density ◽  
Coenzyme A Reductase

A fungal metabolite, ML236B (Compactin), isolated from Penicillium citrinum, is a specific inhibitor of 3-hydroxy-3-methylglutaryl (HMG)-coenzyme A reductase (EC 1.1.1.34). Three ML236B-resistant (ML236Br) mutants, MF-1, MF-2, and MF-3, were isolated from V79 after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The fluctuation test showed 2.2 X 10(-6) mutants per cell per generation of a spontaneous mutation frequency of ML236Br clones. These ML236Br clones showed a four- to fivefold-higher resistance to the drug than did their parental V79. Radioactive acetate, but not mevalonate, incorporation into the sterol fraction increased about 10-fold in ML236Br clones in comparison with that in V79. The cellular level of HMG-coenzyme A reductase in three ML236Br mutants was found to be a few-fold higher than that of V79 when cultured in the presence of lipoproteins. The 125I-labeled low-density lipoprotein-binding assay showed binding activity in three ML236Br clones comparable to that of the parental V79 cells. By contrast, an internalization assay of 125I-labeled low-density lipoprotein into the cells showed significantly reduced activity in three ML236Br clones in comparison with V79.

Chinese hamster cell mutant resistant to ML236B (Compactin) is defective in endocytosis of low-density lipo-protein

1982 ◽  
Vol 2 (11) ◽  
pp. 1354-1361
Author(s):  
A Masuda ◽  
S Akiyama ◽  
M Kuwano
Keyword(s):  
Coenzyme A ◽  
Spontaneous Mutation ◽  
Low Density Lipoprotein ◽  
Chinese Hamster ◽  
Specific Inhibitor ◽  
Density Lipoprotein ◽  
Chinese Hamster Cell ◽  
Cellular Level ◽  
Low Density ◽  
Coenzyme A Reductase

A fungal metabolite, ML236B (Compactin), isolated from Penicillium citrinum, is a specific inhibitor of 3-hydroxy-3-methylglutaryl (HMG)-coenzyme A reductase (EC 1.1.1.34). Three ML236B-resistant (ML236Br) mutants, MF-1, MF-2, and MF-3, were isolated from V79 after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The fluctuation test showed 2.2 X 10(-6) mutants per cell per generation of a spontaneous mutation frequency of ML236Br clones. These ML236Br clones showed a four- to fivefold-higher resistance to the drug than did their parental V79. Radioactive acetate, but not mevalonate, incorporation into the sterol fraction increased about 10-fold in ML236Br clones in comparison with that in V79. The cellular level of HMG-coenzyme A reductase in three ML236Br mutants was found to be a few-fold higher than that of V79 when cultured in the presence of lipoproteins. The 125I-labeled low-density lipoprotein-binding assay showed binding activity in three ML236Br clones comparable to that of the parental V79 cells. By contrast, an internalization assay of 125I-labeled low-density lipoprotein into the cells showed significantly reduced activity in three ML236Br clones in comparison with V79.

Chinese hamster cell mutant, V-C8, a model for analysis of Brca2 function

2006 ◽  
Vol 600 (1-2) ◽  
pp. 79-88 ◽  
Author(s):  
Wouter W. Wiegant ◽  
René M. Overmeer ◽  
Barbara C. Godthelp ◽  
Paul P.W. van Buul ◽  
Małgorzata Z. Zdzienicka
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Cell Mutant

Further characterization of a somatic cell mutant defective in regulation of 3-hydroxy-3-methylglutaryl coenzyme a reductase

1988 ◽  
Vol 14 (6) ◽  
pp. 527-539 ◽  
Author(s):  
Dennis Peffley ◽  
Jon Miyake ◽  
Sherry Leonard ◽  
Charles Gunten ◽  
Michael Sinensky
Keyword(s):  
Somatic Cell ◽  
Coenzyme A ◽  
Cell Mutant ◽  
Coenzyme A Reductase

A novel type of X-ray-sensitive Chinese hamster cell mutant with radioresistant DNA synthesis and hampered DNA double-strand break repair

1995 ◽  
Vol 337 (2) ◽  
pp. 119-129 ◽  
Author(s):  
Gerald W.C.T Verhaegh ◽  
Wim Jongmans ◽  
Bruno Morolli ◽  
Nicolaas G.J Jaspers ◽  
Govert P van der Schans ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Chinese Hamster ◽  
Strand Break ◽  
Chinese Hamster Cell ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Cell Mutant ◽  
X Ray ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals A somatic cell mutant with a kinetically altered 3-hydroxy-3-methylglutaryl coenzyme A reductase

FEBS Letters ◽  
1985 ◽  
Vol 186 (2) ◽  
pp. 219-223 ◽  
Author(s):  
Michael Sinensky ◽  
Judy Logel ◽  
Robin Schnitzer-Polokoff ◽  
Rae Ann Norcross
Keyword(s):  
Somatic Cell ◽  
Coenzyme A ◽  
Cell Mutant ◽  
Coenzyme A Reductase
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