Temperature-sensitive Chinese hamster cell mutant with a defect in glycoprotein synthesis: Accumulation of the EGF receptor in the endoplasmic reticulum and the role of the glucose-regulated protein GRP78

1988 ◽  
Vol 136 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Jean-Jacques Feige ◽  
Gilbert-A. Keller ◽  
Immo E. Scheffler
Keyword(s):  
Endoplasmic Reticulum ◽  
Egf Receptor ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Temperature Sensitive ◽  
Cell Mutant ◽  
Glycoprotein Synthesis ◽  
Glucose Regulated Protein

Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells

1988 ◽  
Vol 8 (10) ◽  
pp. 4063-4070
Author(s):  
A J Dorner ◽  
M G Krane ◽  
R J Kaufman
Keyword(s):  
Endoplasmic Reticulum ◽  
Chinese Hamster Ovary ◽  
Heterologous Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Glycosylation Site ◽  
Ovary Cells ◽  
Tissue Plasminogen ◽  
Stable Association

GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells expressing a tissue plasminogen activator (tPA) variant which lacks potential N-linked glycosylation site sequences because of mutagenesis. The expression of variant tPA resulted in elevated levels of GRP78 and its stable association with tPA. The introduction of antisense GRP78 genes resulted in a two- to threefold reduction in GRP78 levels compared with those of the original cells. Cells with reduced levels of GRP78 secreted two- to threefold-higher levels of tPA activity. tPA expressed in these cells displayed reduced association with GRP78, and a greater proportion was processed to the mature form and secreted. These results demonstrate that reduction of GRP78 level can improve the secretion of an associated protein.

Cloning of a human S-phase cell cycle gene: use of transient expression for screening

1987 ◽  
Vol 7 (2) ◽  
pp. 775-779
Author(s):  
A Fainsod ◽  
G Diamond ◽  
M Marcus ◽  
F H Ruddle
Keyword(s):  
Cell Cycle ◽  
Genomic Library ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
S Phase ◽  
Phase Cell ◽  
Cell Cycle Gene ◽  
Temperature Sensitive ◽  
Specific Cell ◽  
Restrictive Temperature

We report here the cloning of a human cell cycle gene capable of complementing a temperature-sensitive (ts) S-phase cell cycle mutation in a Chinese hamster cell line. Cloning was performed as follows. A human genomic library in phage lambda containing 600,000 phages was screened with labeled cDNA synthesized from an mRNA fraction enriched for the specific cell cycle gene message. Plaques containing DNA inserts which hybridized to the cDNA were picked, and their DNAs were assayed for transient complementation in DNA transformation experiments. The transient complementation assay we developed is suitable for most cell cycle genes and indeed for many genes whose products are required for cell proliferation. Of 845 phages screened, 1 contained an insert active in transient complementation of the ts cell cycle mutation. Introduction of this phage into the ts cell cycle mutant also gave rise to stable transformants which grew normally at the restrictive temperature for the ts mutant cells.

Chinese hamster cell mutant, V-C8, a model for analysis of Brca2 function

2006 ◽  
Vol 600 (1-2) ◽  
pp. 79-88 ◽  
Author(s):  
Wouter W. Wiegant ◽  
René M. Overmeer ◽  
Barbara C. Godthelp ◽  
Paul P.W. van Buul ◽  
Małgorzata Z. Zdzienicka
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Cell Mutant

A novel type of X-ray-sensitive Chinese hamster cell mutant with radioresistant DNA synthesis and hampered DNA double-strand break repair

1995 ◽  
Vol 337 (2) ◽  
pp. 119-129 ◽  
Author(s):  
Gerald W.C.T Verhaegh ◽  
Wim Jongmans ◽  
Bruno Morolli ◽  
Nicolaas G.J Jaspers ◽  
Govert P van der Schans ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Chinese Hamster ◽  
Strand Break ◽  
Chinese Hamster Cell ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Cell Mutant ◽  
X Ray ◽  
Dna Double Strand Break ◽  
Break Repair
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