Assignment of dioxin-inducible cytochrome P-450 gene family to Chinese hamster chromosome 4

1985 ◽  
Vol 11 (4) ◽  
pp. 391-395
Author(s):  
C. Edgar Hildebrand ◽  
Raymond L. Stallings ◽  
Frank J. Gonzalez ◽  
Daniel W. Nebert
Keyword(s):  
Gene Family ◽  
Chinese Hamster ◽  
Chromosome 4 ◽  
Cytochrome P 450 ◽  
Chinese Hamster Chromosome

Provisional assignment ofMPI, PKM2, PGM3, andME1 to Chinese hamster chromosome 4

1984 ◽  
Vol 10 (1) ◽  
pp. 109-111 ◽  
Author(s):  
Raymond L. Stallings ◽  
Gerald M. Adair ◽  
Michael J. Siciliano
Keyword(s):  
Chinese Hamster ◽  
Chromosome 4 ◽  
Chinese Hamster Chromosome

Chromosome polymorphism involving heterochromatic blocks in Chinese hamster chromosome 9

1984 ◽  
Vol 38 (4) ◽  
pp. 257-264 ◽  
Author(s):  
F.A. Ray ◽  
M.F. Bartholdi ◽  
P.M. Kraemer ◽  
L.S. Cram
Keyword(s):  
Chinese Hamster ◽  
Chromosome 9 ◽  
Chromosome Polymorphism ◽  
Chinese Hamster Chromosome

GNAI3, GNAT2, AMPD2, GSTM are clustered in 120 kb of Chinese hamster Chromosome 1q

1996 ◽  
Vol 7 (6) ◽  
pp. 429-432 ◽  
Author(s):  
B. Baron ◽  
M. A. Fernandez ◽  
S. Carignon ◽  
F. Toledo ◽  
G. Buttin ◽  
...  
Keyword(s):  
Chinese Hamster ◽  
Chromosome 1Q ◽  
Chinese Hamster Chromosome

scholarly journals The TRI11 Gene of Fusarium sporotrichioides Encodes a Cytochrome P-450 Monooxygenase Required for C-15 Hydroxylation in Trichothecene Biosynthesis

1998 ◽  
Vol 64 (1) ◽  
pp. 221-225 ◽  
Author(s):  
Nancy J. Alexander ◽  
Thomas M. Hohn ◽  
Susan P. McCormick
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Family ◽  
Gene Cluster ◽  
Biosynthetic Pathway ◽  
Fusarium Sporotrichioides ◽  
Significant Similarity ◽  
Dna Fragment ◽  
Cytochrome P 450 ◽  
Cluster Sequence

ABSTRACT Several genes in the trichothecene biosynthetic pathway ofFusarium sporotrichioides have been shown to reside in a gene cluster. Sequence analysis of a cloned DNA fragment located 3.8 kb downstream from TRI5 has led to the identification of the TRI11 gene. The nucleotide sequence ofTRI11 predicts a polypeptide of 492 residues (M r = 55,579) with significant similarity to members of the cytochrome P-450 superfamily. TRI11 is most similar to several fungal cytochromes P-450 (23 to 27% identity) but is sufficiently distinct to define a new cytochrome P-450 gene family, designated CYP65A1. Disruption of TRI11 results in an altered trichothecene production phenotype characterized by the accumulation of isotrichodermin, a trichothecene pathway intermediate. The evidence suggests that TRI11 encodes a C-15 hydroxylase involved in trichothecene biosynthesis.

Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5

1982 ◽  
Vol 2 (10) ◽  
pp. 1220-1228
Author(s):  
S Dana ◽  
J J Wasmuth
Keyword(s):  
Human Chromosome ◽  
Mammalian Species ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Gene Organization ◽  
Deletion Mapping ◽  
Chromosome 2 ◽  
Chromosome 5 ◽  
Human Chromosome 5 ◽  
Chinese Hamster Chromosome

Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal protein S14) or chr. Cytogenetic and biochemical analyses of spontaneous segregants isolated by using these unique selective pressures have enabled us to determine the order and regional location of the leuS, hexB, emtB, and chr genes on human chromosome 5. These segregants arise primarily by terminal deletions of various portions of the long arm of chromosome 5. Our results indicate that the order of at least three of these genes is the same on human chromosome 5 and Chinese hamster chromosome 2. Thus, there appears to be extensive homology between Chinese hamster chromosome 2 and human chromosome 5, which represents an extreme example of the conservation of gene organization between very divergent mammalian species. In addition, these hybrids and selective conditions provide a very simple and quantitative means to assess the potency of various agents suspected of inducing gross chromosomal damage.

scholarly journals Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity

2005 ◽  
Vol 387 (2) ◽  
pp. 343-353 ◽  
Author(s):  
Jun EGUCHI ◽  
Jun WADA ◽  
Kazuyuki HIDA ◽  
Hong ZHANG ◽  
Takashi MATSUOKA ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Gene Family ◽  
Mrna Expression ◽  
Adhesion Molecule ◽  
Chinese Hamster ◽  
Cell Aggregation ◽  
Cdna Subtraction ◽  
Aggregation Assay ◽  
Oletf Rats ◽  
Type Variable

Few cell adhesion molecules have been reported to be expressed in mature adipocytes, and the significance of cell adhesion process in adipocyte biology is also unknown. In the present study, we identified ACAM (adipocyte adhesion molecule), a novel homologue of the CTX (cortical thymocyte marker in Xenopus) gene family. ACAM cDNA was isolated during PCR-based cDNA subtraction, and its mRNA was shown to be up-regulated in WATs (white adipose tissues) of OLETF (Otsuka Long–Evans Tokushima fatty) rats, an animal model for Type II diabetes and obesity. ACAM, 372 amino acids in total, has a signal peptide, V-type (variable) and C2-type (constant) Ig domains, a single transmembrane segment and a cytoplasmic tail. The amino acid sequence in rat is highly homologous to mouse (94%) and human (87%). ACAM mRNA was predominantly expressed in WATs in OLETF rats, and increased with the development of obesity until 30 weeks of age, which is when the peak of body mass is reached. Western blot analysis revealed that ACAM protein, approx. 45 kDa, was associated with plasma membrane fractions of mature adipocytes isolated from mesenteric and subdermal adipose deposits of OLETF rats. Up-regulation of ACAM mRNAs in obesity was also shown in WATs of genetically obese db/db mice, diet-induced obese ICR mice and human obese subjects. In primary cultured mouse and human adipocytes, ACAM mRNA expression was progressively up-regulated during differentiation. Several stably transfected Chinese-hamster ovary K1 cell lines were established, and the quantification of ACAM mRNA and cell aggregation assay revealed that the degree of homophilic aggregation correlated well with ACAM mRNA expression. In summary, ACAM may be the critical adhesion molecule in adipocyte differentiation and development of obesity.

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