An autoradiographic study of RNA synthesis during maturation and germination of pollen grains ofHyoscyamus niger

PROTOPLASMA ◽  
1982 ◽  
Vol 111 (3) ◽  
pp. 177-188 ◽  
Author(s):  
Thomas L. Reynolds ◽  
V. Raghavan
Keyword(s):  
Rna Synthesis ◽  
Pollen Grains ◽  
Autoradiographic Study ◽  
Germination Of Pollen

scholarly journals snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
K. Zienkiewicz ◽  
E. Bednarska
Keyword(s):  
Pollen Development ◽  
Rna Synthesis ◽  
Generative Cell ◽  
Pollen Grains ◽  
Strong Relationship ◽  
Nuclear Bodies ◽  
Cajal Body ◽  
Sm Proteins ◽  
Hyacinthus Orientalis

The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells ofHyacinthus orientalisL.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identified as Cajal bodies. In all cases, a strong accumulation of snRNP-indicating molecules including TMG cap, Sm proteins and U2 snRNA, was observed in the examined nuclear bodies. In contrast to their number the size of the identified structures did not change significantly during pollen development. In the microspore and the vegetative cell of pollen grains CBs were more numerous than in the generative cell. At later stages of pollen development, a drastic decrease in CB number was observed and, just before anthesis, a complete lack of these structures was indicated in both pollen nuclei. On the basis of these results, as well as our previous studies, we postulate a strong relationship between Cajal body numbers and the levels of RNA synthesis and splicing machinery elements in microspore and developing pollen cells.

Autoradiographic study of RNA synthesis in isolated cells in culture from chick embryo spinal ganglia

1970 ◽  
Vol 106 (4) ◽  
pp. 615-626 ◽  
Author(s):  
M. Sensenbrenner ◽  
J. Treska-Ciesielski ◽  
Z. Lodin ◽  
P. Mandel
Keyword(s):  
Chick Embryo ◽  
Rna Synthesis ◽  
Autoradiographic Study ◽  
Spinal Ganglia ◽  
Isolated Cells ◽  
Cells In Culture
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