An autoradiographic study of RNA synthesis in isolated salivary glands ofDrosophila hydei. I. Autoradiographic studies

1965 ◽  
Vol 116 (1) ◽  
pp. 65-87 ◽  
Author(s):  
Gail Arnold
Keyword(s):  
Salivary Glands ◽  
Rna Synthesis ◽  
Autoradiographic Study

Studies on Nucleolar RNA Synthesis in Drosophila Melanogaster

1972 ◽  
Vol 11 (3) ◽  
pp. 689-697
Author(s):  
H. M. KRIDER ◽  
W. PLAUT
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Glands ◽  
Rna Synthesis ◽  
First Generation ◽  
Nucleolus Organizer ◽  
Fifth Generation ◽  
Autoradiographic Analysis ◽  
Temporally Correlated ◽  
Nucleolar Rna ◽  
Nucleolus Organizers

The influence of conditions resulting in bobbed phenotypes on nucleolar RNA synthesis and the formation of constrictions at nucleolus organizers was examined in larval tissues of Drosophila melanogaster. By means of [3H]uridine incorporation and autoradiographic analysis, a mutation at the bobbed locus was shown to limit the rate of nucleolar RNA synthesis in salivary glands of XO larvae. The formation of constrictions at the organizer sites of a 4-nucleolus-organizer stock was monitored in dividing neuroblast cells stained with acridine orange. Loss of the ribosomal cistrons had been reported by other workers when such stocks were maintained for several generations. In the first generation in our work, constrictions were visible at only 2 of the 4 nucleolus organizers. This situation persisted until the fifth generation, when constrictions appeared at all 4 of the organizer sites. An increase in the rate of nucleolar RNA synthesis in the salivary glands was temporally correlated with the appearance of the extra constrictions. We interpret these observations to mean that 2 of the organizers of the 4-nucleolus-organizer stock were caused to function through the loss of ribosomal RNA cistrons; thus the functional status of an organizer would appear to be subject to control.

Inhibition of RNA synthesis in salivary glands of by 5′-methylthioadenosine

1976 ◽  
Vol 73 (3) ◽  
pp. 600-606 ◽  
Author(s):  
Ronald E. Law ◽  
Ralph M. Sinibaldi ◽  
Michael R. Cummings ◽  
Adolph J. Ferro
Keyword(s):  
Salivary Glands ◽  
Rna Synthesis

Autoradiographic study of RNA synthesis in isolated cells in culture from chick embryo spinal ganglia

1970 ◽  
Vol 106 (4) ◽  
pp. 615-626 ◽  
Author(s):  
M. Sensenbrenner ◽  
J. Treska-Ciesielski ◽  
Z. Lodin ◽  
P. Mandel
Keyword(s):  
Chick Embryo ◽  
Rna Synthesis ◽  
Autoradiographic Study ◽  
Spinal Ganglia ◽  
Isolated Cells ◽  
Cells In Culture

scholarly journals Chromosomal basis of dosage compensation in Drosophila: I. Cellular autonomy of hyperactivity of the male X-chromosome in salivary glands and sex differentiation

1969 ◽  
Vol 14 (2) ◽  
pp. 137-150 ◽  
Author(s):  
S. C. Lakhotia ◽  
A. S. Mukherjee
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Glands ◽  
X Chromosome ◽  
Metabolic Activity ◽  
Dosage Compensation ◽  
Rna Synthesis ◽  
Sex Differentiation ◽  
Chromosome 3 ◽  
Normal Male ◽  
Developmental Physiology

Morphology and the rate of RNA synthesis of the X-chromosome in XX/XO mosaic larval salivary glands of Drosophila melanogaster have been examined. For this purpose the unstable ring-X was utilized to produce XX and XO nuclei in the same pair of glands. The width of the X-chromosome and the left arm of the 3rd chromosome (3L) of larval salivary glands was measured and the rate of RNA synthesis by them was studied upon the use of [3H]uridine autoradiography in such XX (female) and XO (male) nuclei developing in a female background (i.e. otherwise genotypically XX). In such mosaic glands the width of the single X-chromosome of male nuclei is nearly as great as that of the paired two X's of female nuclei, as is also the case in normal male (X Y) and female (XX). The single X of male nuclei synthesizes RNA at a rate equal to that of the paired two X's of female nuclei and nearly twice that of an unpaired X of XX nuclei. Neither the developmental physiology of the sex nor the proportion of XO nuclei in a pair of mosaic salivary glands of an XX larva has any influence on these two characteristics of the male X-chromosome.It is suggested that dosage compensation in Drosophila is achieved chiefly, if not fully, by a hyperactivity of the male X, in contrast to the single X inactivation in female mammals, that this hyperactivity of the male X is expressed visibly in the morphology and metabolic activity of the X-chromosome in the larval salivary glands of the male, and that this hyperactivity and therefore dosage compensation in Drosophila in general is not dependent on sex-differentiation, but is a function of the doses of the X-chromosome itself.

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