The 35S-CaMV promoter is silent during early embryogenesis but activated during nonembryogenic sporophytic development in microspore culture

PROTOPLASMA ◽  
1999 ◽  
Vol 208 (1-4) ◽  
pp. 257-264 ◽  
Author(s):  
J. B. M. Custers ◽  
S. C. H. J. Snepvangers ◽  
H. J. Jansen ◽  
L. Zhang ◽  
M. M. van Lookeren Campagne
Keyword(s):  
Microspore Culture ◽  
Early Embryogenesis ◽  
Sporophytic Development

scholarly journals Microspore culture of Zantedeschia aethiopica: The role of monosaccharides in sporophytic development

2011 ◽  
Vol 10 (50) ◽  
pp. 10287-10292 ◽  
Author(s):  
Wang Shimin ◽  
Li Xiaolu ◽  
Yang Lili ◽  
Wu Hongzhi ◽  
Zheng Sixiang ◽  
...  
Keyword(s):  
Microspore Culture ◽  
Zantedeschia Aethiopica ◽  
Sporophytic Development

Role of Auxin in Early Embryogenesis of Plants

2014 ◽  
Vol 48 (4) ◽  
pp. 371-380
Author(s):  
Song Lizhen ◽  
Wang Yi ◽  
Yang Qinghua ◽  
Cheng Youfa
Keyword(s):  
Early Embryogenesis

Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus

1994 ◽  
Vol 13 (5) ◽  
pp. 267-271 ◽  
Author(s):  
Jan B. M. Custers ◽  
Jan H. G. Cordewener ◽  
Yvonne Nöllen ◽  
Hans J. M. Dons ◽  
Michiel M. Van Lockeren Campagne
Keyword(s):  
Brassica Napus ◽  
Sporophytic Development

scholarly journals A Comparison of the Structure and Function of the Highly Homologous Maize Antioxidant Cu/Zn Superoxide Dismutase Genes, sod4 and Sod4A

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 317-328 ◽  
Author(s):  
Sheri P Kernodle ◽  
John G Scandalios
Keyword(s):  
Structure And Function ◽  
Specific Response ◽  
Stem Tissue ◽  
Mrna Accumulation ◽  
Regulatory Motifs ◽  
Homologous Sequences ◽  
Developmental Profiles ◽  
And Function ◽  
Differential Tissue ◽  
Sporophytic Development

Abstract Two highly similar cytosolic Cu/Zn Sod (Sod4 and Sod4A) genes have been isolated from maize. Sod4A contains eight exons and seven introns. The Sod4 partial sequence contains five introns. The introns in both genes are located in the same position and have highly homologous sequences in several regions. The largest intron (>1200 bp) interrupts the 5′ leader sequence. The presence of different regulatory motifs in the promoter region of each gene may indicate distinct responses to various conditions. Zymogram and RNA blot analyses show that Sod4 and Sod4A are expressed in all tissues of the maize plant. The developmental profiles of Sod4 and Sod4A mRNA accumulation differ in scutella during sporophytic development. RNA blot analysis of the respective Sod mRNAs indicates a differential, tissue-specific response of each gene to certain stressors. RNA isolated from stem tissue of ethephon-treated seedlings shows an increase in the Sod4 but not the Sod4A transcript while there is no change in transcripts of either gene in leaves or roots. There is differential mRNA accumulation between the two genes in leaf and stem tissue of paraquat-treated seedlings. Other agents that can cause oxidative stress were also tested for differential expression of the genes.

pitkinD, a Novel Gain-of-Function Enhancer of Position-Effect Variegation, Affects Chromatin Regulation During Oogenesis and Early Embryogenesis in Drosophila

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1227-1244 ◽  
Author(s):  
Steffi Kuhfittig ◽  
János Szabad ◽  
Gunnar Schotta ◽  
Jan Hoffmann ◽  
Endre Máthé ◽  
...  
Keyword(s):  
Germ Line ◽  
Position Effect ◽  
Early Embryogenesis ◽  
Position Effect Variegation ◽  
Chromatin Regulation ◽  
Gain Of Function ◽  
Position Effects ◽  
Dominant Female ◽  
Effect Variegation ◽  
Female Germ Line

Abstract The vast majority of the >100 modifier genes of position-effect variegation (PEV) in Drosophila have been identified genetically as haplo-insufficient loci. Here, we describe pitkinDominant (ptnD), a gain-of-function enhancer mutation of PEV. Its exceptionally strong enhancer effect is evident as elevated spreading of heterochromatin-induced gene silencing along euchromatic regions in variegating rearrangements. The ptnD mutation causes ectopic binding of the SU(VAR)3-9 heterochromatin protein at many euchromatic sites and, unlike other modifiers of PEV, it also affects stable position effects. Specifically, it induces silencing of white+ transgenes inserted at a wide variety of euchromatic sites. ptnD is associated with dominant female sterility. +/+ embryos produced by ptnD/+ females mated with wild-type males die at the end of embryogenesis, whereas the ptnD/+ sibling embryos arrest development at cleavage cycle 1-3, due to a combined effect of maternally provided mutant product and an early zygotic lethal effect of ptnD. This is the earliest zygotic effect of a mutation so far reported in Drosophila. Germ-line mosaics show that ptn+ function is required for normal development in the female germ line. These results, together with effects on PEV and white+ transgenes, are consistent with the hypothesis that the ptn gene plays an important role in chromatin regulation during development of the female germ line and in early embryogenesis.

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