The cytotoxic effect of fowlpox virus on primary human amniotic cell cultures

1968 ◽  
Vol 24 (1-2) ◽  
pp. 137-147 ◽  
Author(s):  
J. W. Burnett ◽  
T. E. Frothingham
Keyword(s):  
Cell Cultures ◽  
Cytotoxic Effect ◽  
Fowlpox Virus ◽  
Amniotic Cell

A supernumerary microchromosome in amniotic cell cultures and talipes equinovarus in a live born female

2008 ◽  
Vol 26 (5) ◽  
pp. 477-480 ◽  
Author(s):  
Razia S. Muneer ◽  
Jeannie R. Himes ◽  
René M. Payne-Howell ◽  
Lora M. Thompson ◽  
Owen M. Rennert
Keyword(s):  
Cell Cultures ◽  
Talipes Equinovarus ◽  
Amniotic Cell

Development of biotechnology and assessment of antitumor properties of dietary supplements of tissue origin on cell cultures

2021 ◽  
pp. 33-34
Author(s):  
Наталья Александровна Кольберг ◽  
Сергей Леонидович Тихонов ◽  
Наталья Валерьевна Тихонова ◽  
Светлана Александровна Леонтьева
Keyword(s):  
Dietary Supplements ◽  
Tumor Cells ◽  
Dietary Supplement ◽  
Cell Cultures ◽  
Mammalian Cells ◽  
Broiler Chickens ◽  
Cytotoxic Effect ◽  
Antitumor Properties ◽  
Tissue Origin ◽  
Mcf 7

Разработана технология БАД из фабрициевой сумки цыплят-бройлеров. Доказано, что полученная БАД на нарушает жизнеспособность клеток млекопитающих и не проявляет цитотоксических свойств на метаболизм здоровых клеток, что свидетельствует о ее безопасности. Доказан выраженный цитотоксический эффект БАД на опухолевые клетки линии MCF-7. The technology of dietary supplements from the factory bag of broiler chickens has been developed. It was proved that the obtained dietary supplement does not violate the viability of mammalian cells and does not exhibit cytotoxic properties on the metabolism of healthy cells, which indicates its safety. A pronounced cytotoxic effect of dietary supplements on MCF-7 tumor cells has been proven.

scholarly journals Cytotoxic Effect of Trabectedin In Human Adrenocortical Carcinoma Cell Lines and Primary Cells

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 928 ◽  
Author(s):  
Andrea Abate ◽  
Elisa Rossini ◽  
Sara Anna Bonini ◽  
Martina Fragni ◽  
Deborah Cosentini ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cell Cultures ◽  
Adrenocortical Carcinoma ◽  
Cytotoxic Effect ◽  
Alkylating Agents ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
High Cytotoxicity

Mitotane is the only drug approved for the treatment of adrenocortical carcinoma (ACC). The regimen to be added to mitotane is a chemotherapy including etoposide, doxorubicin, and cisplatin. This pharmacological approach, however, has a limited efficacy and significant toxicity. Evidence indicates that ACC seems to be sensitive to alkylating agents. Trabectedin is an anti-tumor drug that acts as an alkylating agent with a complex mechanism of action. Here, we investigated whether trabectedin could exert a cytotoxic activity in in vitro cell models of ACC. Cell viability was evaluated by MTT assay on ACC cell lines and primary cell cultures. The gene expression was evaluated by q-RT-PCR, while protein expression and localization were studied by Western blot and immunocytochemistry. Combination experiments were performed to evaluate their interaction on ACC cell line viability. Trabectedin demonstrated high cytotoxicity at sub-nanomolar concentrations in ACC cell lines and patient-derived primary cell cultures. The drug was able to reduce /β catenin nuclear localization, although it is unclear whether this effect is involved in the observed cytotoxicity. Trabectedin/mitotane combination exerted a synergic cytotoxic effect in NCI-H295R cells. Trabectedin has antineoplastic activity in ACC cells. The synergistic cytotoxic activity of trabectedin with mitotane provides the rationale for testing this combination in a clinical study.

scholarly journals Cytotoxic effect of multinucleation in HeLa cell cultures associated with the presence of Vir plasmid inEscherichia colistrains

1989 ◽  
Vol 58 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Eric Oswald ◽  
Jean Rycke ◽  
Jean François Guillot ◽  
Roger Boivin
Keyword(s):  
Hela Cell ◽  
Cell Cultures ◽  
Cytotoxic Effect

Further examples of trisomy-20 mosaicism in amniotic cell cultures

1985 ◽  
Vol 5 (2) ◽  
pp. 159-162 ◽  
Author(s):  
M. S. Grewal ◽  
M. A. C. Ridler ◽  
J. C. K. Barber
Keyword(s):  
Cell Cultures ◽  
Amniotic Cell

Additive cytotoxic effect of cisplatin and X-irradiation on human glioma cell cultures derived from biopsy-tissue

2000 ◽  
Vol 126 (12) ◽  
pp. 711-716 ◽  
Author(s):  
Fabian Fehlauer ◽  
Angeliqué D. Barten-Van Rijbroek ◽  
Lukas J. A. Stalpers ◽  
Sieger Leenstra ◽  
Jan Lindeman ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Glioma Cell ◽  
Cytotoxic Effect ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Biopsy Tissue ◽  
X Irradiation

Small supernumerary chromosomes in amniotic cell cultures (two cases)

Pathology ◽  
1984 ◽  
Vol 16 (1) ◽  
pp. 108
Author(s):  
L. Voullaire ◽  
M. Susman ◽  
G. Allen
Keyword(s):  
Cell Cultures ◽  
Supernumerary Chromosomes ◽  
Amniotic Cell

scholarly journals Isolation of motile Aeromonas spp. from fish and their cytotoxic effect on Vero cell cultures

2002 ◽  
Vol 52 (1) ◽  
pp. 3-10
Author(s):  
Karabasil Nedjeljko ◽  
Asanin Ruzica ◽  
Baltic Milan ◽  
Teodorovic V. ◽  
Dimitrijevic Mirjana
Keyword(s):  
Vero Cell ◽  
Cell Cultures ◽  
Cytotoxic Effect

scholarly journals Immunological Activities of Isoprinosine Inhibition on Viral Infections Inhuman

2019 ◽  
Vol 16 (04) ◽  
pp. 773-778
Author(s):  
Hashim Ali Abdualmeer Al-Sherees ◽  
Sumaya NajimAbedali Al-khateeb ◽  
Fadhil Hussain Nasir Al-Muhannak
Keyword(s):  
Antiviral Activity ◽  
Cell Cultures ◽  
A549 Cell ◽  
Cytotoxic Effect ◽  
Viral Infections ◽  
Colorimetric Assay ◽  
Morphological Changes ◽  
Antiviral Drug ◽  
Virus Multiplication ◽  
Mtt Colorimetric Assay

Isoprinosine is a combination of inosine used as antiviral drug without effect on viral particle itself, but instead only and acts as on immunostimulant and also acts indirectly by activation of immune cells. Aim of this study was to determine level of interferon-alpha (INF-α) with parainfluenza viruses HPIV-2, and adenoviruses HAdV-2 replication. In the present study, cytotoxic effect of isoprinosine was assessed using A549 cell line exposed to different concentrations of compound (isoprinosine: 50-800μg/mL) for 48 hours. Cytotoxic effect was examined visually using light, inverted microscopy Olympus CK2 under 400x magnification and by the MTT colorimetric assay. The yield re­duction assay (YRA), which evaluates the ability of the isoprinosine (50-800 μg/mL) to inhibit virus multiplication in cell cultures, was applied. The cytopathic effect of the virus was evaluated 48 h after infection of A549 cell cultures with viruses by means of light, inverted microsco­py. The YRA method was used to determine the 50% end point (IC50) in the presence of Isoprinosine with the controlled one. MTT cytotoxicity assay confirmed microscopic observations. There were no morphological changes, as assessed visually, in cell cultures treated with isoprinosine. After conducting the experi­ments and analyzing the results we noticed that higher concentrations of isoprinosine strongly inhibited multiplication of all viruses. HPIV-2 and HAdV-2 showed the highest sensitivity to the antiviral activity of isoprinosine as compared with the control, however, increasing concentrations of isoprinosine up to 800 μg /ml slightly enhanced the antiviral activity of 400 μg/ml isoprinosine. Our study was conducted that HAdV-2 and HPIV-2 have the highest sensitivity to the antiviral activity of isoprinosine from all tested viral strains.

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