scholarly journals Isolation of motile Aeromonas spp. from fish and their cytotoxic effect on Vero cell cultures

2002 ◽  
Vol 52 (1) ◽  
pp. 3-10
Author(s):  
Karabasil Nedjeljko ◽  
Asanin Ruzica ◽  
Baltic Milan ◽  
Teodorovic V. ◽  
Dimitrijevic Mirjana
Keyword(s):  
Vero Cell ◽  
Cell Cultures ◽  
Cytotoxic Effect

Development of biotechnology and assessment of antitumor properties of dietary supplements of tissue origin on cell cultures

2021 ◽  
pp. 33-34
Author(s):  
Наталья Александровна Кольберг ◽  
Сергей Леонидович Тихонов ◽  
Наталья Валерьевна Тихонова ◽  
Светлана Александровна Леонтьева
Keyword(s):  
Dietary Supplements ◽  
Tumor Cells ◽  
Dietary Supplement ◽  
Cell Cultures ◽  
Mammalian Cells ◽  
Broiler Chickens ◽  
Cytotoxic Effect ◽  
Antitumor Properties ◽  
Tissue Origin ◽  
Mcf 7

Разработана технология БАД из фабрициевой сумки цыплят-бройлеров. Доказано, что полученная БАД на нарушает жизнеспособность клеток млекопитающих и не проявляет цитотоксических свойств на метаболизм здоровых клеток, что свидетельствует о ее безопасности. Доказан выраженный цитотоксический эффект БАД на опухолевые клетки линии MCF-7. The technology of dietary supplements from the factory bag of broiler chickens has been developed. It was proved that the obtained dietary supplement does not violate the viability of mammalian cells and does not exhibit cytotoxic properties on the metabolism of healthy cells, which indicates its safety. A pronounced cytotoxic effect of dietary supplements on MCF-7 tumor cells has been proven.

scholarly journals Cytotoxic Effect of Trabectedin In Human Adrenocortical Carcinoma Cell Lines and Primary Cells

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 928 ◽  
Author(s):  
Andrea Abate ◽  
Elisa Rossini ◽  
Sara Anna Bonini ◽  
Martina Fragni ◽  
Deborah Cosentini ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cell Cultures ◽  
Adrenocortical Carcinoma ◽  
Cytotoxic Effect ◽  
Alkylating Agents ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
High Cytotoxicity

Mitotane is the only drug approved for the treatment of adrenocortical carcinoma (ACC). The regimen to be added to mitotane is a chemotherapy including etoposide, doxorubicin, and cisplatin. This pharmacological approach, however, has a limited efficacy and significant toxicity. Evidence indicates that ACC seems to be sensitive to alkylating agents. Trabectedin is an anti-tumor drug that acts as an alkylating agent with a complex mechanism of action. Here, we investigated whether trabectedin could exert a cytotoxic activity in in vitro cell models of ACC. Cell viability was evaluated by MTT assay on ACC cell lines and primary cell cultures. The gene expression was evaluated by q-RT-PCR, while protein expression and localization were studied by Western blot and immunocytochemistry. Combination experiments were performed to evaluate their interaction on ACC cell line viability. Trabectedin demonstrated high cytotoxicity at sub-nanomolar concentrations in ACC cell lines and patient-derived primary cell cultures. The drug was able to reduce /β catenin nuclear localization, although it is unclear whether this effect is involved in the observed cytotoxicity. Trabectedin/mitotane combination exerted a synergic cytotoxic effect in NCI-H295R cells. Trabectedin has antineoplastic activity in ACC cells. The synergistic cytotoxic activity of trabectedin with mitotane provides the rationale for testing this combination in a clinical study.

scholarly journals Production of yellow fever virus in microcarrier-based Vero cell cultures

Vaccine ◽  
2009 ◽  
Vol 27 (46) ◽  
pp. 6420-6423 ◽  
Author(s):  
Marta Cristina O. Souza ◽  
Marcos S. Freire ◽  
Erica A. Schulze ◽  
Luciane P. Gaspar ◽  
Leda R. Castilho
Keyword(s):  
Vero Cell ◽  
Yellow Fever ◽  
Cell Cultures ◽  
Yellow Fever Virus ◽  
Fever Virus

scholarly journals Cytotoxic effect of multinucleation in HeLa cell cultures associated with the presence of Vir plasmid inEscherichia colistrains

1989 ◽  
Vol 58 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Eric Oswald ◽  
Jean Rycke ◽  
Jean François Guillot ◽  
Roger Boivin
Keyword(s):  
Hela Cell ◽  
Cell Cultures ◽  
Cytotoxic Effect

scholarly journals Recovery of bluetongue virus serogroup from sera collected for a serological survey from apparently healthy cattle, from the Sudan

1986 ◽  
Vol 96 (3) ◽  
pp. 529-533 ◽  
Author(s):  
E. M. E. Abu Elzein
Keyword(s):  
Vero Cell ◽  
Cell Cultures ◽  
Bluetongue Virus ◽  
Complement Fixation ◽  
Serological Survey ◽  
Agar Gel ◽  
Sole Purpose ◽  
Healthy Cattle ◽  
Apparently Healthy

SUMMARYVirus of the bluetongue (BT) serogroup was recovered from 11% of cattle sera collected from apparently healthy animals in Khartoum Province for the sole purpose of screening for BT antibodies. Since these sera did not contain BT antibodies, the donor cattle could have been scored as BT free in the serological survey.Virus was initially isolated in chicken embryos inoculated intravascularly, and was further adapted to Vero cell cultures. Isolates were identified as belonging to the BT serogroup using the agar gel immunodiffusion (AGID) and complement fixation (CF) tests.The results indicated that cattle in the Sudan could harbour BT virus without showing symptoms of the disease. Such an observation necessitates further work to clarify the role of cattle in the epidemiology of BT in the Sudan.

The cytotoxic effect of fowlpox virus on primary human amniotic cell cultures

1968 ◽  
Vol 24 (1-2) ◽  
pp. 137-147 ◽  
Author(s):  
J. W. Burnett ◽  
T. E. Frothingham
Keyword(s):  
Cell Cultures ◽  
Cytotoxic Effect ◽  
Fowlpox Virus ◽  
Amniotic Cell
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