The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco explants at a large range of concentrations

Planta ◽  
1990 ◽  
Vol 180 (3) ◽  
pp. 410-415 ◽  
Author(s):  
M. J. M. Smulders ◽  
E. J. W. Visser ◽  
A. F. Croes ◽  
G. J. Wullems
Keyword(s):  
Large Range ◽  
Naphthaleneacetic Acid ◽  
Flower Bud ◽  
Bud Regeneration

scholarly journals Effects of the Developmental State of the Tissue on the Competence for Flower Bud Regeneration in Pedicel Explants of Tobacco

1990 ◽  
Vol 92 (3) ◽  
pp. 582-586 ◽  
Author(s):  
Marinus J. M. Smulders ◽  
Eric J. W. Visser ◽  
Wim M. Van der Krieken ◽  
Anton F. Croes ◽  
George J. Wullems
Keyword(s):  
Developmental State ◽  
Flower Bud ◽  
Bud Regeneration ◽  
Pedicel Explants

Regeneration of peanut (Arachis hypogaea) plantlets by in vitro culture of immature leaves

1981 ◽  
Vol 59 (5) ◽  
pp. 826-830 ◽  
Author(s):  
L. A. Mroginski ◽  
K. K. Kartha ◽  
J. P. Shyluk
Keyword(s):  
In Vitro Culture ◽  
Arachis Hypogaea ◽  
Environmental Conditions ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Bud Regeneration ◽  
Arachis Hypogaea L ◽  
Murashige And Skoog Medium ◽  
Germinated Seeds

The in vitro regeneration of buds, shoots, and roots from immature leaves of 3- to 5-day-old peanut (Arachis hypogaea L. cv. Colorado Manfredi) seedlings was studied under defined nutritional, hormonal, and environmental conditions. The first two leaves (2–5 mm in length) removed from aseptically germinated seeds were cultured on Murashige and Skoog medium containing vitamins as in B5 medium and 0.8% agar, supplemented with 12 combinations of naphthaleneacetic acid (NAA) (0.01 to 4 mg/L) and benzyladenine (BA) (1 and 3 mg/L). Bud regeneration occurred in all hormone combinations, but the maximum number of buds was regenerated at a concentration of 1 mg/L each of NAA and BA. Although bud regeneration was maximum with 2- to 5-mm-long leaflets, some success was also obtained with leaflets 8–13 mm long. However, no buds were regenerated when fully expanded leaflets were cultured.Development of buds into shoots was readily achieved by transferring regenerated buds into fresh medium containing 0.01 mg/L NAA and 1 mg/L BA. A few roots were induced to grow when callus with buds was also transferred to medium devoid of hormones. So far, bud regeneration from immature leaves has been induced in vitro in 5 of the 10 cultivars tested.

REGENERATION OF TREMA ORIENTALIS (BLUME) LINN.: EFFECT OF GROWTH REGULATORS, CULTURE CONDITIONS, AND AGE AND SOURCE OF EXPLANTS

1995 ◽  
Vol 43 (4) ◽  
pp. 391-395 ◽  
Author(s):  
G.R. Rout ◽  
S. Samantaray ◽  
P. Das
Keyword(s):  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Mature Trees ◽  
Regeneration Rate ◽  
Bud Regeneration ◽  
Shoot Bud ◽  
Shoot Bud Regeneration ◽  
Trema Orientalis

Optimal conditions for high frequency shoot bud regeneration from leaf callus of Trema orientalis (Blume) Linn. were studied. The regeneration rate was controlled by the growth regulators, the age and the source of the explants, and the illumination conditions. Irrespective of illumination conditions, shoot bud regeneration was achieved only in media containing benzyladenine (BA) + α-naphthaleneacetic acid (NAA) combinations, with the best results being obtained in the presence of 2.5 mg/1 BA and 0.25–0.5 mg/1 NAA. The morphogenic response was less frequent in the calluses derived from leaf explants of the mature trees compared to those of the in vitro-grown seedlings. The rate of shoot bud regeneration was more pronounced in the cultures maintained for 4 weeks in the light (16-h photoperiod) than the cultures incubated in the dark. Regenerated shoots were rooted on the medium containing 1/2 strength basal Murashige and Skoog (MS) salts supplemented with 0.01 mg/1 NAA or indole-3-butyric acid (IBA). The rooted plantlets were established in the greenhouse.

High-Frequency Shoot Regeneration From Flower Bud Derived Callus of Gymnostachyum Febrifugum Benth., an Endemic Medicinal Plant to the Western Ghats

2021 ◽  
Author(s):  
Silpa P ◽  
Dennis Thuruthiyil Thomas
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Flower Bud ◽  
The Family ◽  
Endemic Medicinal Plant ◽  
Dichlorophenoxy Acetic Acid ◽  
The Western Ghats

Abstract Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an efficient protocol for high-frequency flower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the inflorescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1- naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3- butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

Influence of the Developing Fruit on Aging of the Floral Stalk Tissue with Respect to Flower Bud Regeneration in vitro

1986 ◽  
Vol 125 (1-2) ◽  
pp. 61-68 ◽  
Author(s):  
A.F. Croes ◽  
Rob Derksen ◽  
Anke Kemp ◽  
Henk van Wezel ◽  
G.W.M. Barendse
Keyword(s):  
Flower Bud ◽  
Bud Regeneration ◽  
Regeneration In Vitro

scholarly journals In Vitro Rooting and Greenhouse Acclimatization of Veltheimia bracteata and V. capensis Shoots

HortScience ◽  
1996 ◽  
Vol 31 (7) ◽  
pp. 1229-1230 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Overall Survival ◽  
Potassium Salt ◽  
Leaf Explants ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Flower Bud ◽  
Single Leaf ◽  
Shoot Initiation ◽  
Survival Percentage

Shoot initiation and multiplication were obtained in vitro from immature flower bud and leaf explants of Veltheimia bracteata Bak. `Lemon Flame' and from leaf explants of V. bracteata `Rosalba' cultured on a Murashige and Skoog (MS) medium supplemented with sucrose at 30 g•L–1, and either 8.87 μm BA plus 0.54 μm NAA or 8.87 μm BA plus 5.40 μm NAA. Shoot initiation and multiplication was obtained from a single leaf explant of Veltheimia capensis (L.) DC. on MS medium with 8.87 μm BA plus 0.54 μm NAA. Shoots of the three genotypes rooted on subculture to medium with 0.0, 4.14, or 8.29 μm K-IBA or 0.0, 4.46, or 8.92 μm K-NAA. Maximal rooting was 98% for V. bracteata `Lemon Flame', 95% for V. bracteata `Rosalba', and 98% for V. capensis, from medium with 4.46 μm KNAA. Rooted shoots were acclimatized for 3 to 4 weeks. Overall survival percentage was 69% for V. bracteata `Lemon Flame', 65% for V. bracteata `Rosalba', and 83% for V. capensis. Chemical names used: 6-benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthaleneacetic acid (K-NAA); 1-naphthaleneacetic acid (NAA).

scholarly journals Naphthaleneacetic Acid and Ethephon Are Florigenic in the Biennial Apple Cultivars Golden Delicious and York Imperial

HortScience ◽  
2013 ◽  
Vol 48 (6) ◽  
pp. 742-746 ◽  
Author(s):  
Steven McArtney ◽  
Duane Greene ◽  
Tory Schmidt ◽  
Rongcai Yuan
Keyword(s):  
Floral Development ◽  
Naphthaleneacetic Acid ◽  
Summer Program ◽  
Flower Bud ◽  
Biennial Bearing ◽  
Time Period ◽  
Golden Delicious ◽  
Apple Cultivars ◽  
Return Bloom ◽  
Flower Bud Formation

‘Golden Delicious’ and ‘York Imperial’ are apple cultivars that are prone to develop a biennial bearing habit. A successful chemical thinning program with carbaryl plus 6-benzyladenine applied at the 10-mm fruit diameter stage reduced cropload and increased return bloom of ‘York Imperial’, although the improvement in return bloom resulting from chemical thinning was insufficient to ensure a commercial cropload in the year after treatment (fewer than 10% of spurs developing flowers). A chemical thinning program with multiple applications of a naphthaleneacetic acid (NAA) and ethephon mixture during the period from 36 to 73 days after bloom increased return bloom of ‘York Imperial’ trees to commercially acceptable levels (25% or greater of spurs flowering). NAA applied during the period from 50 to 100 days after bloom (summer NAA program) or from 110 to 140 days after bloom (preharvest NAA program) increased return bloom of ‘Golden Delicious’. When aminoethoxyvinylglycine (AVG) was included with the first NAA spray in a summer program, the efficacy was reduced, indicating that ethylene may be partly involved in the florigenic activity of NAA. Dissection of ‘Golden Delicious’ buds sampled from three locations (Asheville, NC; Amherst, MA; Wenatchee, WA) at ≈14-day intervals beginning 50 days after bloom indicated that the time of floral transition (doming of the meristem apex) occurred during the period from 65 to 105 days after bloom at each location. Thus, NAA applications in a summer program for return bloom coincided with the period when floral determination normally occurred. Preharvest NAA programs effectively promoted return bloom in the experiments where a summer NAA program was also effective. These responses indicate that NAA can trigger floral development within vegetative buds relatively late in the summer and outside of the time period when it is generally believed possible to influence flower bud formation.

Role of Benzyladenine in Flower Bud Regeneration in vitro: Timing of Hormone Action

1991 ◽  
Vol 138 (2) ◽  
pp. 168-171 ◽  
Author(s):  
W.M. van der Krieken ◽  
A.F. Croes ◽  
G.W.M. Barendse ◽  
G.J. Wullems
Keyword(s):  
Hormone Action ◽  
Flower Bud ◽  
Bud Regeneration ◽  
Regeneration In Vitro

Small Computer System for Micrograph Analysis

1981 ◽  
Vol 39 ◽  
pp. 270-271
Author(s):  
J. F. Hainfeld ◽  
J. S. Wall
Keyword(s):  
Large Range ◽  
Small Computer ◽  
Black And White ◽  
Contour Maps ◽  
Points Of Interest ◽  
Computer Aided Analysis ◽  
Specialized Hardware ◽  
Additional Memory ◽  
Selection Of ◽  
Publication Quality

Cost reduction and availability of specialized hardware for image processing have made it reasonable to purchase a stand-alone interactive work station for computer aided analysis of micrographs. Some features of such a system are: 1) Ease of selection of points of interest on the micrograph. A cursor can be quickly positioned and coordinates entered with a switch. 2) The image can be nondestructively zoomed to a higher magnification for closer examination and roaming (panning) can be done around the picture. 3) Contrast and brightness of the picture can be varied over a very large range by changing the display look-up tables. 4) Marking items of interest can be done by drawing circles, vectors or alphanumerics on an additional memory plane so that the picture data remains intact. 5) Color pictures can easily be produced. Since the human eye can detect many more colors than gray levels, often a color encoded micrograph reveals many features not readily apparent with a black and white display. Colors can be used to construct contour maps of objects of interest. 6) Publication quality prints can easily be produced by taking pictures with a standard camera of the T.V. monitor screen.

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