Effect of culture density on cyclic-AMP responses of rabbit aortic smooth-muscle cells to hormonal stimulation

1981 ◽  
Vol 1 (8) ◽  
pp. 643-649 ◽  
Author(s):  
Lindsey Needham ◽  
Trevor J. Franklin ◽  
Sobhy R. Ayad
Keyword(s):  
Smooth Muscle ◽  
Cyclic Amp ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Hormonal Stimulation ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Culture Density

Forskolin and isoproterenol effect discrete responses on epidermal growth factor induced DNA synthesis in aortic smooth muscle cells

1993 ◽  
Vol 71 (10-11) ◽  
pp. 800-805 ◽  
Author(s):  
S. L. Venance ◽  
B. M. Bennett ◽  
S. C. Pang
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Thymidine Incorporation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Epidermal Growth

Defining the mechanisms regulating the proliferation of vascular smooth muscle is necessary to better understand the pathogenesis of atherosclerosis and hypertension. In the present investigation, we examined the effects of incubation with forskolin or isoproterenol on the proliferation of cultured rat aortic smooth muscle cells. Forskolin, a direct activator of adenylate cyclase, and isoproterenol, a β-adrenergic agonist, increased intracellular cyclic AMP levels in a concentration-dependent manner, subsequent to a 5-min exposure. Isobutylmethylxanthine at 100 μM attenuated epidermal growth factor stimulated DNA synthesis by 35% without affecting intracellular cyclic AMP levels. Forskolin dose-dependently augmented this inhibition. In contrast, a 24-h exposure of cells to isoproterenol resulted in a biphasic effect on growth factor stimulated thymidine incorporation. Both forskolin and isoproterenol attenuated thymidine incorporation to the same degree up to 12 h poststimulation, the onset of S phase. By 16 h poststimulation, [3H]thymidine incorporation in smooth muscle cells treated with isoproterenol was significantly enhanced by 50%, whereas forskolin treatment continued to attenuate DNA synthesis by 40%. Somewhat surprisingly, this disparity in effect on DNA synthesis was evident in spite of heterologous desensitization to rechallenge by either forskolin or isoproterenol subsequent to a 24-h incubation with either drug. These results suggest that the isoproterenol enhancement of epidermal growth factor stimulated DNA synthesis in rat aortic smooth muscle cells may be cyclic AMP independent.Key words: smooth muscle cells, proliferation, cyclic AMP.

scholarly journals The Effect of Theophylline and Cyclic AMP on Fetal Bovine Aortic Smooth Muscle Cells in Culture

1985 ◽  
Vol 13 (3) ◽  
pp. 665-668
Author(s):  
Michitaka NAITO ◽  
Kan-ichi ASAI ◽  
Kazuaki SHIBATA ◽  
Masafumi KUAUYA ◽  
Chiaki FUNAKI ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cyclic Amp ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Cells In Culture ◽  
Fetal Bovine
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