Cyclic AMP and Protein Kinase in the Spontaneously Hypertensive Rat Aorta and Tissue-Cultured Aortic Smooth Muscle Cells

1976 ◽  
Vol 13 (6) ◽  
pp. 361-373 ◽  
Author(s):  
Howard Sands ◽  
Diane Sinclair ◽  
James Mascali
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Smooth Muscle Cells ◽  
Spontaneously Hypertensive Rat ◽  
Muscle Cells ◽  
Rat Aorta ◽  
Spontaneously Hypertensive ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Antiproliferative action of protein kinase C in cultured rabbit aortic smooth muscle cells

1987 ◽  
Vol 173 (2) ◽  
pp. 504-514 ◽  
Author(s):  
Ken-Ichi Kariya ◽  
Yasuo Fukumoto ◽  
Terutaka Tsuda ◽  
Takeshi Yamamoto ◽  
Yasuhiro Kawahara ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Kinase C ◽  
Antiproliferative Action

Regulation of prostacyclin production by [Ca2+]i and protein kinase C in aortic smooth muscle cells

1992 ◽  
Vol 263 (4) ◽  
pp. E800-E806
Author(s):  
A. C. Erbrich ◽  
D. J. Church ◽  
M. B. Vallotton ◽  
U. Lang
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Prolonged Incubation ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Kinase C ◽  
Prostacyclin Production

The respective roles of protein kinase C (PKC) and of cytosolic free Ca2+ concentration ([Ca2+]i) in prostacyclin synthesis were investigated in aortic smooth muscle cells by using A23187 and phorbol 12-myristate 13-acetate (PMA) to bypass the hormonal receptor. Exposure of the cells to A23187 markedly increased prostacyclin production, which was not affected by the PKC inhibitor staurosporine or by PKC depletion after prolonged incubation (48 h) of cells with PMA. The increase in [Ca2+]i induced by A23187 did not affect membranous or cytosolic PKC activity in control and PMA-stimulated cells. Activation of PKC by PMA, a weak stimulant of prostacyclin production by itself, strongly potentiated A23187-induced prostacyclin production, as well as that induced by the calcium-mobilizing hormone arginine vasopressin (AVP). The potentiating effect persisted for 30 min after the removal of PMA. However, this "memory" effect was not due to sustained levels of membranous PKC activity but probably to the prolonged influence of PKC-induced phosphorylation(s). Taken together, our results suggest that, although an increase in [Ca2+]i is sufficient for inducing prostacyclin production in rat aortic smooth muscle cells, activation of PKC is necessary for AVP-induced prostacyclin production in this same tissue.

Protein kinase C-mediated intracellular alkalinization in rat and rabbit aortic smooth muscle cells

1987 ◽  
Vol 141 (3) ◽  
pp. 503-506 ◽  
Author(s):  
N.Raju Danthuluri ◽  
Bradford C. Berk ◽  
Tommy A. Brock ◽  
Edward J. Cragoe ◽  
Richard C. Deth
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Kinase C ◽  
Intracellular Alkalinization

Incidence of multinucleated and polyploid aortic smooth muscle cells cultured from different age groups of spontaneously hypertensive rats

1992 ◽  
Vol 70 (11) ◽  
pp. 1496-1501 ◽  
Author(s):  
Robert M. K. W. Lee ◽  
Roop B. Conyers ◽  
Chiu-Yin Kwan
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Spontaneously Hypertensive Rats ◽  
Age Groups ◽  
Muscle Cells ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Aortic Smooth Muscle Cells ◽  
Positive Correlation ◽  
Aortic Smooth Muscle

Cell size and incidence of multinucleated, polyploid cells in cultured aortic smooth muscle cells from different age groups of spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto rats (WKY) were compared. Smooth muscle cells from SHR were generally larger than those from WKY, and the percentage of multinucleated smooth muscle cells was always higher in SHR than WKY in the three age groups of rats studied (3–4, 10–12, and 28–30 weeks). In smooth muscle cells from the 3- to 4-week group, there was a positive correlation between cell diameter and the percentage of multinucleated smooth muscle cells. Microdensitometric measurements also showed that the incidence of polyploid smooth muscle cells was always higher in SHR than WKY in the three age groups. There was a positive correlation between DNA density and nuclear area measurements in all the age groups of SHR and WKY. We conclude that cultured aortic smooth muscle cells from different age groups of SHR and WKY contained heterogeneous populations of cells and that, under our culture conditions, the polyploidy of the smooth muscle cells found in vivo was maintained in the SHR and WKY.Key words: hypertension, cultured smooth muscle cells, aorta, polyploidy.

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