Cyclic AMP inhibits c-Ha-ras protooncogene expression, and DNA synthesis in rat aortic smooth muscle cells

1993 ◽  
Vol 49 (6-7) ◽  
pp. 567-570 ◽  
Author(s):  
D. N. Sadhu ◽  
K. S. Ramos
Keyword(s):  
Smooth Muscle ◽  
Cyclic Amp ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Forskolin and isoproterenol effect discrete responses on epidermal growth factor induced DNA synthesis in aortic smooth muscle cells

1993 ◽  
Vol 71 (10-11) ◽  
pp. 800-805 ◽  
Author(s):  
S. L. Venance ◽  
B. M. Bennett ◽  
S. C. Pang
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Cyclic Amp ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Thymidine Incorporation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Epidermal Growth

Defining the mechanisms regulating the proliferation of vascular smooth muscle is necessary to better understand the pathogenesis of atherosclerosis and hypertension. In the present investigation, we examined the effects of incubation with forskolin or isoproterenol on the proliferation of cultured rat aortic smooth muscle cells. Forskolin, a direct activator of adenylate cyclase, and isoproterenol, a β-adrenergic agonist, increased intracellular cyclic AMP levels in a concentration-dependent manner, subsequent to a 5-min exposure. Isobutylmethylxanthine at 100 μM attenuated epidermal growth factor stimulated DNA synthesis by 35% without affecting intracellular cyclic AMP levels. Forskolin dose-dependently augmented this inhibition. In contrast, a 24-h exposure of cells to isoproterenol resulted in a biphasic effect on growth factor stimulated thymidine incorporation. Both forskolin and isoproterenol attenuated thymidine incorporation to the same degree up to 12 h poststimulation, the onset of S phase. By 16 h poststimulation, [3H]thymidine incorporation in smooth muscle cells treated with isoproterenol was significantly enhanced by 50%, whereas forskolin treatment continued to attenuate DNA synthesis by 40%. Somewhat surprisingly, this disparity in effect on DNA synthesis was evident in spite of heterologous desensitization to rechallenge by either forskolin or isoproterenol subsequent to a 24-h incubation with either drug. These results suggest that the isoproterenol enhancement of epidermal growth factor stimulated DNA synthesis in rat aortic smooth muscle cells may be cyclic AMP independent.Key words: smooth muscle cells, proliferation, cyclic AMP.

Effect of pituitary adenylate cyclase activating polypeptide on vasopressin-induced proliferation of aortic smooth muscle cells: comparison with vasoactive intestinal polypeptide

1993 ◽  
Vol 71 (3-4) ◽  
pp. 156-161 ◽  
Author(s):  
Yutaka Oiso ◽  
Jun Kotoyori ◽  
Takashi Murase ◽  
Yoshiaki Ito ◽  
Osamu Kozawa
Keyword(s):  
Cell Cycle ◽  
Smooth Muscle ◽  
Adenylate Cyclase ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Vasoactive Intestinal Polypeptide ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Pituitary Adenylate Cyclase

Pituitary adenylate cyclase activating polypeptide (PACAP) inhibited dose dependently the DNA synthesis stimulated by arginine vasopressin (AVP) in cultured rat aortic smooth muscle cells (SMC). The inhibition was cell cycle dependent and the maximum inhibition was observed when added at the late G1 phase of the cell cycle. Vasoactive intestinal polypeptide (VIP), which shows a considerable homology with PACAP, also inhibited dose dependently the AVP-induced DNA synthesis in a cell cycle dependent manner. The maximum inhibition was also observed at the late G1 phase. The patterns of both the dose-dependent inhibitions were similar, and the inhibition by a combination of PACAP and VIP was not additive. PACAP stimulated dose dependently cAMP accumulation in aortic SMC. VIP also stimulated cAMP accumulation, and the accumulation by a combination of PACAP and VIP was not additive. Both PACAP and VIP had little effect on phosphoinositide hydrolysis in these cells. The suppression of the AVP-induced DNA synthesis by PACAP or VIP was enhanced by 3-isobutyl-1-methylxanthine, an inhibitor for phosphodiesterases. Dibutyryl cAMP, but not 8-bromo-cGMP, inhibited the AVP-induced DNA synthesis, and a combination of PACAP and dibutyryl cAMP was not additive. [Ac-Tyr1, D-Phe2]growth hormone-releasing factor, an antagonist for VIP receptor, reversed the inhibitory effect of PACAP on the AVP-induced DNA synthesis. These results suggest that PACAP has an antiproliferative effect on aortic SMC at the late G1 phase of the cell cycle through cAMP production, and that PACAP and VIP inhibit the AVP-induced DNA synthesis by a common mechanism.Key words: pituitary adenylate cyclase activating polypeptide, vasoactive intestinal polypeptide, arginine vasopressin, DNA synthesis, aortic smooth muscle cells.

scholarly journals Cholesterol Enhances Platelet-Derived Growth Factor-BB-Induced [Ca 2+ ] i and DNA Synthesis in Rat Aortic Smooth Muscle Cells

Hypertension ◽  
1997 ◽  
Vol 29 (1) ◽  
pp. 326-333 ◽  
Author(s):  
Agapios Sachinidis ◽  
Min Liu ◽  
Artur-Aron Weber ◽  
Claudia Seul ◽  
Volker Harth ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Muscle Cells ◽  
Platelet Derived Growth Factor ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle
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