Improved yeast tolerance to ethanol-induced death by centrifugates of dense cell suspensions incubated with ethanol

1987 ◽  
Vol 9 (9) ◽  
pp. 643-646 ◽  
Author(s):  
R. C. Nabais ◽  
I. S�-Correia ◽  
J. M. Novais
Keyword(s):  
Cell Suspensions ◽  
Dense Cell ◽  
Tolerance To Ethanol

Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications

1984 ◽  
Vol 30 (10) ◽  
pp. 1229-1238 ◽  
Author(s):  
T. O'Reilly ◽  
S. Rosendal ◽  
D. F. Niven
Keyword(s):  
Cell Suspensions ◽  
Test Strips ◽  
Taxonomic Analysis ◽  
Dense Cell ◽  
Haemophilus Species ◽  
Taxonomic Implications ◽  
Species Taxon ◽  
Porcine Origin

Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both strips allowed adequate differentiation of closely related organisms. Numerical taxonomic analysis of the data demonstrated that the majority of the haemophili and actinobacilli studied could be placed in one of four major clusters; these clusters contained, respectively, the H. pleuropneumoniae – A. pleuropneumoniae strains, the H. parasuis strains, strains belonging to Haemophilus taxon "minor group," and strains belonging to an unusual group of mannitol-positive, urease-negative haemophili. A representative of Haemophilus species taxon C and an unusual Actinobacillus isolate appeared to be comparatively unrelated to organisms in the four major clusters. Although it may, on occasion, be difficult to place an unusual isolate in any one particular group, owing to the uncertain taxonomy of some of these organisms, it is concluded that API test strips can serve as useful tools for the characterization and differentiation of porcine haemophili and actinobacilli.

Convenient microscopic tests for yeast viability and sporogenic capability

1970 ◽  
Vol 16 (8) ◽  
pp. 797-798 ◽  
Author(s):  
J. J. Miller
Keyword(s):  
Liquid Phase ◽  
Cell Suspension ◽  
Nutrient Medium ◽  
Cell Suspensions ◽  
Cover Glass ◽  
Yeast Viability ◽  
Dense Cell ◽  
Agar Surface ◽  
Acetate Agar

Drops of cell suspension are seeded on nutrient medium containing 5% purified agar and spread between the agar surface and a cover glass. The liquid phase is absorbed by the concentrated agar, so that the cells are held in situ between glass and agar. After incubation nongerminated cells and micro-colonies are counted microscopically. Sporogenic capability is assessed by seeding very dense cell suspensions on acetate agar and covering with Teflon F.E.P. membrane which is permeable to oxygen.

Electropermeabilization of dense cell suspensions

2007 ◽  
Vol 36 (3) ◽  
pp. 173-185 ◽  
Author(s):  
Gorazd Pucihar ◽  
Tadej Kotnik ◽  
Justin Teissié ◽  
Damijan Miklavčič
Keyword(s):  
Cell Suspensions ◽  
Dense Cell

scholarly journals Hydrogen-Dependent Oxygen Reduction by Homoacetogenic Bacteria Isolated from Termite Guts

2003 ◽  
Vol 69 (2) ◽  
pp. 779-786 ◽  
Author(s):  
Hamadi I. Boga ◽  
Andreas Brune
Keyword(s):  
Oxygen Reduction ◽  
Cell Suspensions ◽  
Organic Substrates ◽  
Acetobacterium Woodii ◽  
Large Capacity ◽  
Oxygen Gradient ◽  
Homoacetogenic Bacteria ◽  
Partial Pressures ◽  
Growth Bands ◽  
Dense Cell

ABSTRACT Although homoacetogenic bacteria are generally considered to be obligate anaerobes, they colonize the intestinal tracts of termites and other environments that are not entirely anoxic in space or time. In this study, we investigated how homoacetogenic bacteria isolated from the hindguts of various termites respond to the presence of molecular oxygen. All strains investigated formed growth bands in oxygen gradient agar tubes under a headspace of H2-CO2. The position of the bands coincided with the oxic-anoxic interface and depended on the O2 partial pressure in the headspace; the position of the bands relative to the meniscus remained stable for more than 1 month. Experiments with dense cell suspensions, performed with Clark-type O2 and H2 electrodes, revealed a large capacity for H2-dependent oxygen reduction in Sporomusa termitida and Sporomusa sp. strain TmAO3 (149 and 826 nmol min−1 mg of protein−1, respectively). Both strains also reduced O2 with endogenous reductants, albeit at lower rates. Only in Acetonema longum did the basal rates exceed the H2-dependent rates considerably (181 versus 28 nmol min−1 mg of protein)−1). Addition of organic substrates did not stimulate O2 consumption in any of the strains. Nevertheless, reductive acetogenesis by cell suspensions of strain TmAO3 was inhibited even at the lowest O2 fluxes, and growth in nonreduced medium occurred only after the bacteria had rendered the medium anoxic. Similar results were obtained with Acetobacterium woodii, suggesting that the results are not unique to the strains isolated from termites. We concluded that because of their tolerance to temporary exposure to O2 at low partial pressures (up to 1.5 kPa in the case of strain TmAO3) and because of their large capacity for O2 reduction, homoacetogens can reestablish conditions favorable for growth by actively removing oxygen from their environment.

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

DEVELOPMENT OF ISOLOGOUS TRANSPLANTS OF CELL SUSPENSIONS OF THE FETAL PANCREAS IN THE RAT

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S97 ◽  
Author(s):  
K. H. Usadel ◽  
U. Schwedes ◽  
U. Leuschner ◽  
K. Schöffling
Keyword(s):  
Cell Suspensions ◽  
Fetal Pancreas
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