Convenient microscopic tests for yeast viability and sporogenic capability

1970 ◽  
Vol 16 (8) ◽  
pp. 797-798 ◽  
Author(s):  
J. J. Miller
Keyword(s):  
Liquid Phase ◽  
Cell Suspension ◽  
Nutrient Medium ◽  
Cell Suspensions ◽  
Cover Glass ◽  
Yeast Viability ◽  
Dense Cell ◽  
Agar Surface ◽  
Acetate Agar

Drops of cell suspension are seeded on nutrient medium containing 5% purified agar and spread between the agar surface and a cover glass. The liquid phase is absorbed by the concentrated agar, so that the cells are held in situ between glass and agar. After incubation nongerminated cells and micro-colonies are counted microscopically. Sporogenic capability is assessed by seeding very dense cell suspensions on acetate agar and covering with Teflon F.E.P. membrane which is permeable to oxygen.

Flat embeddment of monolayer cells, tissue sections, and other cellular materials attached onto glass substrate

1990 ◽  
Vol 48 (3) ◽  
pp. 766-767
Author(s):  
Jeffrey P. Chang ◽  
Jaang J. Wang
Keyword(s):  
Present Report ◽  
Cellular Materials ◽  
Cover Glass ◽  
Tissue Sections ◽  
Embedding Technique ◽  
Exfoliated Cells ◽  
Black Paper ◽  
Cell Concentrations ◽  
Flat Embedding

Flat embeddment of certain specimens for electron microscopy is necessary for three classes of biological materials: namely monolayer cells, tissue sections of paraffin or plastics, as well as cell concentrations, exfoliated cells, and cell smears. The present report concerns a flat-embedding technique which can be applied to all these three classes of materials and which is a modified and improved version of Chang's original methodology.Preparation of coverglasses and microslides. Chemically cleaned coverglasses, 11 × 22 mm or other sizes, are laid in rows on black paper. Ink-mark one coner for identifying the spray-side of the glass for growing cells. Lightly spray with Teflon monomer (Heddy/Contact Inductries, Paterson, NO 07524, U.S.A.) from a pressurized can. Bake the sprayed glasses at 500°F for 45 min on Cover-Glass Ceramic Racks (A. Thomas Co. Philadelphia), for Teflon to polymerize.Monolayer Cells. After sterilization, the Teflon-treated coverglasses, with cells attached, are treated or fixed in situ in Columbia staining dishes (A. Thomas Co., Philadelphia) for subsequent processing.

scholarly journals Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 118
Author(s):  
Manoj Kumar Tripathi ◽  
Niraj Tripathi ◽  
Sushma Tiwari ◽  
Gyanendra Tiwari ◽  
Nishi Mishra ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Nutrient Medium ◽  
Normal Growth ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Embryonic Axis ◽  
Santalum Album ◽  
Relative Growth ◽  
Santalum Album L ◽  
Embryogenic Tissues

Santalum album (L.) is a prized tropical tree species of high therapeutic and industrial importance. The wood of these naturally grown plants is extensively harvested to acquire therapeutically important metabolite santalol and be used for additional functions such as in wood statuette industries. Due to high demand, it is crucial to maintain a sufficient plant population. An easy protocol for establishing cell suspension culture initiated from the loose embryogenic callus mass of sandalwood was realized by shifting 6–8-week-old morphogenic calli acquired from the mature embryonic axis and cotyledon explant cultures in fluid media. The asynchronous embryogenic cultures were sloughed with clumps of flourishing cell clumps and embryos of various progressive phases along with diffident non-embryogenic tissues. The frequency of embryo proliferation was evidenced to determinethe expansion pace of embryogenic masses under diverse conditions. The intonation of initiation and creation of cell suspension was under the directive of the influence of exogenous plant growth regulators amended in the nutrient medium at different concentrations and combinations. Maximum relative growth rate (386%) and clumps/embryoids in elevated integers (321.44) were accomplished on MS nutrient medium fortified with 2.0 mg L−1 2,4-D in association with 0.5 mg L−1 BA and 30.0 g L−1 sucrose raised from mature embryonic axis-derived calli. Plantlet regeneration in higher frequency (84.43%) was evidenced on MS medium amended with 1.0 mg L−1 each of TDZ and GA3 in conjunction with 0.5 mg L−1 NAA and 20.0 g L−1 sucrose. Mature embryonic axis-derived calli were found to be constantly better than mature cotyledon-derived calli for raising profitable and reproducible cell suspension cultures. Regenerants displayed normal growth and morphology and were founded successfully in the external environment after hardening.

scholarly journals In Situ FTIR Reactor for Monitoring Gas-Phase Products during a (Photo)catalytic Reaction in the Liquid Phase

2018 ◽  
Vol 90 (24) ◽  
pp. 14586-14592 ◽  
Author(s):  
Igor Telegeiev ◽  
Oumaima Thili ◽  
Adrien Lanel ◽  
Philippe Bazin ◽  
Yoann Levaque ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Gas Phase ◽  
Catalytic Reaction ◽  
In Situ Ftir
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