Interactions between prostaglandin E2 andd-ala2-met-enkephalinamide on adenylate cyclase activity in the guinea-pig superior cervical ganglion

1988 ◽  
Vol 13 (9) ◽  
pp. 797-802 ◽  
Author(s):  
Maria Enrica Ferretti ◽  
Pier Giorgio Borasio ◽  
Carla Biondi ◽  
Antonio Capuzzo ◽  
Elena Fabbri ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Prostaglandin E2 ◽  
Superior Cervical Ganglion ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cervical Ganglion

Prostaglandin E2-binding sites and cAMP production in porcine fundic mucosa

1981 ◽  
Vol 241 (4) ◽  
pp. G313-G320
Author(s):  
B. L. Tepperman ◽  
B. D. Soper
Keyword(s):  
Adenylate Cyclase ◽  
Prostaglandin E2 ◽  
Binding Site ◽  
Binding Sites ◽  
Biologically Active ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Scatchard Analysis ◽  
Fundic Mucosa ◽  
Stimulation Of

Biologically active [3H]prostaglandin E2 (PGE2) bound rapidly and specifically to membrane fractions from hog fundic mucosa. Optimal binding occurred in the 30,000-g membrane preparation at 37 degrees C (pH 5.0). Scatchard analysis of specific PgE2 binding revealed the presence of a heterogeneous population of binding sites with Kd values and binding site concentrations of approximately 1 X 10(-9) M and 1 fmol/mg prot and 2 X 10(-8) M and 20 fmol/mg prot, respectively. Specific binding was inhibited by the following agents in descending order of potency: PGE1, PGA2, PGD2, 6-keto-PGF1 alpha, and thromboxane B2. Trypsin treatment or boiling reduced or abolished specific PGE2 binding. PGE2 stimulated cAMP formation in the 2,500-g fraction, with an approximate Km of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not b of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not b of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not be localized in these fractions in vitro.

Effects of PHI on vasoactive intestinal peptide receptors and adenylate cyclase activity in lung membranes. A comparison in man, rat, mouse and guinea pig

1982 ◽  
Vol 4 (5) ◽  
pp. 241-250 ◽  
Author(s):  
Patrick Robberecht ◽  
Kazuhiko Tatemoto ◽  
Pierre Chatelain ◽  
Magali Waelbroeck ◽  
Myriam Delhaye ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Vasoactive Intestinal Peptide ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Peptide Receptors

Cytochemical demonstration of Adenylate cyclase activity in basal compartment of Guinea pig testis

1983 ◽  
Vol 50 (1-2) ◽  
pp. 105-108
Author(s):  
Rita Pascolini ◽  
Daniela Vagnetti ◽  
Marinella Marinelli ◽  
Sergio Lorvik
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cytochemical Demonstration
Sign in / Sign up

Export Citation Format

Share Document