Supra-additive stimulation of adenylate cyclase activity by prostaglandin E2 andD-Ala2-met-enkephalinamide in the guinea-pig superior cervical ganglion: Role of Mg2+ ions

1989 ◽  
Vol 14 (12) ◽  
pp. 1181-1186 ◽  
Author(s):  
Pier Giorgio Borasio ◽  
Carla Biondi ◽  
Maria Enrica Ferretti ◽  
Elena Fabbri ◽  
Maria Cristina Pareschi
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Prostaglandin E2 ◽  
Superior Cervical Ganglion ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cervical Ganglion ◽  
Stimulation Of

Interactions between prostaglandin E2 andd-ala2-met-enkephalinamide on adenylate cyclase activity in the guinea-pig superior cervical ganglion

1988 ◽  
Vol 13 (9) ◽  
pp. 797-802 ◽  
Author(s):  
Maria Enrica Ferretti ◽  
Pier Giorgio Borasio ◽  
Carla Biondi ◽  
Antonio Capuzzo ◽  
Elena Fabbri ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Prostaglandin E2 ◽  
Superior Cervical Ganglion ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cervical Ganglion

Prostaglandin E2-binding sites and cAMP production in porcine fundic mucosa

1981 ◽  
Vol 241 (4) ◽  
pp. G313-G320
Author(s):  
B. L. Tepperman ◽  
B. D. Soper
Keyword(s):  
Adenylate Cyclase ◽  
Prostaglandin E2 ◽  
Binding Site ◽  
Binding Sites ◽  
Biologically Active ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Scatchard Analysis ◽  
Fundic Mucosa ◽  
Stimulation Of

Biologically active [3H]prostaglandin E2 (PGE2) bound rapidly and specifically to membrane fractions from hog fundic mucosa. Optimal binding occurred in the 30,000-g membrane preparation at 37 degrees C (pH 5.0). Scatchard analysis of specific PgE2 binding revealed the presence of a heterogeneous population of binding sites with Kd values and binding site concentrations of approximately 1 X 10(-9) M and 1 fmol/mg prot and 2 X 10(-8) M and 20 fmol/mg prot, respectively. Specific binding was inhibited by the following agents in descending order of potency: PGE1, PGA2, PGD2, 6-keto-PGF1 alpha, and thromboxane B2. Trypsin treatment or boiling reduced or abolished specific PGE2 binding. PGE2 stimulated cAMP formation in the 2,500-g fraction, with an approximate Km of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not b of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not b of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not be localized in these fractions in vitro.

scholarly journals Role of prostaglandins in hypoxia-stimulated erythropoietin production

1985 ◽  
Vol 249 (1) ◽  
pp. C3-C8 ◽  
Author(s):  
A. Kurtz ◽  
W. Jelkmann ◽  
J. Pfeilschifter ◽  
C. Bauer
Keyword(s):  
Arachidonic Acid ◽  
Adenylate Cyclase ◽  
Cell Cultures ◽  
Mesangial Cells ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cyclooxygenase Inhibitor ◽  
Erythropoietin Production ◽  
Stimulation Of

The role of prostaglandins in the mediation of hypoxia-stimulated erythropoietin (Ep) production by cultured rat renal mesangial cells was examined. It was found that an increase in prostaglandin E2 (PGE2) production accompanied the rise in Ep due to hypoxia (2% O2). The hypoxia-stimulated increase in Ep production was abolished in the presence of the cyclooxygenase inhibitor indomethacin (10(-5) M). When PGE2 (10(-6) M was added simultaneously with indomethacin, however, no diminution in hypoxia-stimulated Ep production was observed. Addition of arachidonic acid (AA, 10(-5) M), PGE2 (10(-6) M), or PGI2 (10(-4) M) enhanced Ep production under normoxic conditions (20% O2), while PGF2 alpha (10(-6) M) had no effect on Ep production. AA, PGE2, and PGI2 were found to stimulate adenosine 3',5'-cyclic monophosphate formation by the cultured mesangial cells. Enhancement of adenylate cyclase activity by forskolin (10(-5) M) also increased Ep production in the cell cultures. Our results suggest that hypoxia-stimulated Ep production by cultured mesangial cells is mediated by prostaglandins with subsequent stimulation of adenylate cyclase activity.

Differential effects of prostaglandin E2 on contractions of airway smooth muscle

1989 ◽  
Vol 66 (3) ◽  
pp. 1397-1407 ◽  
Author(s):  
J. M. Madison ◽  
C. A. Jones ◽  
R. M. Sankary ◽  
J. K. Brown
Keyword(s):  
Smooth Muscle ◽  
Adenylate Cyclase ◽  
Prostaglandin E2 ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Tracheal Smooth Muscle ◽  
Inhibitory Effects ◽  
Camp Content

In an in vitro muscle bath, the active tension generated by strips of canine tracheal smooth muscle responding to cumulative additions of either histamine (10(-8) to 10(-3) M) or acetylcholine (10(-9) to 10(-3) M) was measured in the absence and presence of prostaglandin E2 (PGE2) (10(-6) to 10(-5) M). When contractile responses of equal magnitude were compared, the contractions elicited by acetylcholine were resistant to the inhibitory effects of PGE2, relative to comparable contractions elicited by histamine. To assess the role of adenylate cyclase in determining the different responses to histamine and acetylcholine in the presence of PGE2, we assayed adenylate cyclase activity in membranes prepared from canine tracheal smooth muscle and found that acetylcholine, but not histamine, decreased PGE2-stimulated adenylate cyclase activity by 48 +/- 2% (mean +/- SE; n = 5). However, in other experiments, we found that even large pharmacological increases in tissue adenosine 3′,5′-cyclic monophosphate (cAMP) content only partially inhibited muscarinic tone. Also, exogenously applied analogues of cyclic AMP inhibited contractions induced by histamine more effectively than comparable contractions induced by acetylcholine. We concluded that acetylcholine decreased adenylate cyclase activity in membranes prepared from canine tracheal smooth muscle and that this effect may have contributed to, but did not completely account for, the relative resistance of muscarinic contractions to the inhibitory effects of PGE2.

scholarly journals The role of GTP in prostaglandin E1 stimulation of adenylate cyclase in platelet membranes

1983 ◽  
Vol 214 (1) ◽  
pp. 231-234 ◽  
Author(s):  
J M Stein ◽  
B R Martin
Keyword(s):  
Adenylate Cyclase ◽  
Prostaglandin E1 ◽  
Platelet Membrane ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent ◽  
Stimulation Of

Adenylate cyclase activity in platelet membrane preparations was measured in the presence of prostaglandin E1 (PGE1), GTP and a non-hydrolysable analogue of GDP, guanosine 5′-[beta-thio]diphosphate (GDP[beta S]). A dose-dependent inhibition of adenylate cyclase by GDP[beta S] was observed that could be reversed either by adding increased amounts of GTP or of PGE1.

Receptor-mediated supra-additive activation of guinea pig superior cervical ganglion adenylate cyclase: Role of Mn2+ ions and calmodulin

1991 ◽  
Vol 16 (5) ◽  
pp. 583-589 ◽  
Author(s):  
Maria Enrica Ferretti ◽  
Pier Giorgio Borasio ◽  
Carla Biondi ◽  
Anna Lisa Campi ◽  
Maria Cristina Pareschi ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Superior Cervical Ganglion ◽  
Cervical Ganglion

Supra-additive activation of guinea-pig superior cervical ganglion adenylate cyclase by PGE2 andd-Ala2-Met-enkephalinamide: Role of GTP

1990 ◽  
Vol 15 (8) ◽  
pp. 785-789 ◽  
Author(s):  
Carla Biondi ◽  
Pier Giorgio Borasio ◽  
Maria Enrica Ferretti ◽  
Maria Cristina Pareschi
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Superior Cervical Ganglion ◽  
Cervical Ganglion
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