Comparison of the effects of histamine and tolazoline on adenylate cyclase activity from guinea pig heart

1975 ◽  
Vol 18 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Ira Weinryb ◽  
Inge M. Michel
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Guinea Pig Heart

scholarly journals Differential effects of non-ionic detergents on microsomal and sarcolemmal adenylate cyclase in cardiac muscle

1978 ◽  
Vol 175 (1) ◽  
pp. 171-180 ◽  
Author(s):  
Prakash V. Sulakhe ◽  
Njanoor Narayanan
Keyword(s):  
Enzyme Activity ◽  
Sarcoplasmic Reticulum ◽  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Protein Ratio ◽  
Guinea Pig Heart ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Ionic Detergent ◽  
Triton X

1. About 4 and 23% of the homogenate adenylate cyclase activity was recovered in the microsomal and sarcolemmal fractions isolated from guinea-pig heart ventricles. 2. Cardiac microsomal adenylate cyclase activity [basal as well as p[NH]ppG (guanyl-5′-yl imidodiphosphate)- and NaF-stimulated] was increased over 2-fold in the presence of Lubrol-PX (0.01–0.1%). 3. The sarcolemmal enzyme, however, showed concentration-dependent inhibition caused by the detergent under all assay conditions, except when p[NH]ppG was included in the assay. In the latter case, the detergent (0.01–0.02%) caused a modest increase (30–45%) in enzyme activity. 4. Another non-ionic detergent, Triton X-100, also stimulated the microsomal cyclase and inhibited the sarcolemmal enzyme. 5. With either membrane fraction, Lubrol-PX solubilized the enzyme when the detergent/membrane protein ratio was 2.5 (μmol of detergent/mg of protein). 6. The findings with homogenate and a washed particulate fraction resembled those obtained with sarcolemma, and those with isolated sarcoplasmic reticulum resembled those with microsomal preparations. 7. p[NH]ppG, and to some extent NaF, protected the detergent-induced inactivation of the enzyme observed at higher detergent concentrations (0.5% Lubrol-PX and 0.05–0.5% Triton X-100). 8. In the absence of detergents, p[NH]ppG increased the basal enzyme activity about 2-fold in microsomal fractions, but did not appreciably stimulate the sarcolemmal enzyme. Isoproterenol, on the other hand, increased the sarcolemmal enzyme activity (>2-fold) in the presence of p[NH]ppG and caused only moderate stimulation (31%) of the microsomal enzyme under these conditions. 9. These findings support the view that, although the bulk of adenylate cyclase resides in heart sarcolemma (plasma membrane), the microsomal activity cannot be accounted for solely by contamination of the microsomal fraction with sarcolemma, as has been suggested by others [Besch, Jones & Watanabe (1976) Circ. Res.39, 586–595; Engelhard, Plut & Storm (1976) Biochim. Biophys. Acta451, 48–61]. Further, the results of this study show that cardiac sarcoplasmic-reticulum membranes possess this enzyme.

Effects of PHI on vasoactive intestinal peptide receptors and adenylate cyclase activity in lung membranes. A comparison in man, rat, mouse and guinea pig

1982 ◽  
Vol 4 (5) ◽  
pp. 241-250 ◽  
Author(s):  
Patrick Robberecht ◽  
Kazuhiko Tatemoto ◽  
Pierre Chatelain ◽  
Magali Waelbroeck ◽  
Myriam Delhaye ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Vasoactive Intestinal Peptide ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Peptide Receptors

Cytochemical demonstration of Adenylate cyclase activity in basal compartment of Guinea pig testis

1983 ◽  
Vol 50 (1-2) ◽  
pp. 105-108
Author(s):  
Rita Pascolini ◽  
Daniela Vagnetti ◽  
Marinella Marinelli ◽  
Sergio Lorvik
Keyword(s):  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cytochemical Demonstration

Evaluation of novel compounds interacting with H2-histamine receptors: Effect on histamine-sensitive adenylate cyclase activity in guinea-pig gastric mucosa

1985 ◽  
Vol 16 (5) ◽  
pp. 291-294 ◽  
Author(s):  
Rosella Micheletti ◽  
Daniela Oliva ◽  
Pietro Belfiore ◽  
Antonio Giachetti ◽  
Simonetta Nicosia
Keyword(s):  
Gastric Mucosa ◽  
Adenylate Cyclase ◽  
Guinea Pig ◽  
Histamine Receptors ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity
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