Differential abundance of simple repetitive sequences in species ofBrassica and relatedBrassicaceae

1994 ◽  
Vol 190 (1-2) ◽  
pp. 21-30 ◽  
Author(s):  
G. B. Poulsen ◽  
G. Kahl ◽  
K. Weising
Keyword(s):  
Repetitive Sequences ◽  
Differential Abundance ◽  
Simple Repetitive Sequences

scholarly journals Repetitive Sequence and Sex Chromosome Evolution in Vertebrates

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Tariq Ezaz ◽  
Janine E. Deakin
Keyword(s):  
Repetitive Dna ◽  
Sex Chromosomes ◽  
Repetitive Sequence ◽  
Chromosome Evolution ◽  
Sex Chromosome ◽  
Repetitive Sequences ◽  
Sex Chromosome Evolution ◽  
Genomic Changes ◽  
Heteromorphic Sex Chromosomes ◽  
Simple Repetitive Sequences

Sex chromosomes are the most dynamic entity in any genome having unique morphology, gene content, and evolution. They have evolved multiple times and independently throughout vertebrate evolution. One of the major genomic changes that pertain to sex chromosomes involves the amplification of common repeats. It is hypothesized that such amplification of repeats facilitates the suppression of recombination, leading to the evolution of heteromorphic sex chromosomes through genetic degradation of Y or W chromosomes. Although contrasting evidence is available, it is clear that amplification of simple repetitive sequences played a major role in the evolution of Y and W chromosomes in vertebrates. In this review, we present a brief overview of the repetitive DNA classes that accumulated during sex chromosome evolution, mainly focusing on vertebrates, and discuss their possible role and potential function in this process.

Simple repetitive sequences and gene expression

2000 ◽  
Vol 34 (3) ◽  
pp. 303-307 ◽  
Author(s):  
A. V. Pisarchik ◽  
N. A. Kartel’
Keyword(s):  
Gene Expression ◽  
Repetitive Sequences ◽  
Simple Repetitive Sequences

scholarly journals Saccharomyces cerevisiae RAD5-encoded DNA repair protein contains DNA helicase and zinc-binding sequence motifs and affects the stability of simple repetitive sequences in the genome.

1992 ◽  
Vol 12 (9) ◽  
pp. 3807-3818 ◽  
Author(s):  
R E Johnson ◽  
S T Henderson ◽  
T D Petes ◽  
S Prakash ◽  
M Bankmann ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Repetitive Sequences ◽  
Dna Helicase ◽  
Gene Arrangement ◽  
Sequence Motif ◽  
Sequence Motifs ◽  
Wild Type ◽  
Dna Repair Gene ◽  
Simple Repetitive Sequences

rad5 (rev2) mutants of Saccharomyces cerevisiae are sensitive to UV light and other DNA-damaging agents, and RAD5 is in the RAD6 epistasis group of DNA repair genes. To unambiguously define the function of RAD5, we have cloned the RAD5 gene, determined the effects of the rad5 deletion mutation on DNA repair, DNA damage-induced mutagenesis, and other cellular processes, and analyzed the sequence of RAD5-encoded protein. Our genetic studies indicate that RAD5 functions primarily with RAD18 in error-free postreplication repair. We also show that RAD5 affects the rate of instability of poly(GT) repeat sequences. Genomic poly(GT) sequences normally change length at a rate of about 10(-4); this rate is approximately 10-fold lower in the rad5 deletion mutant than in the corresponding isogenic wild-type strain. RAD5 encodes a protein of 1,169 amino acids of M(r) 134,000, and it contains several interesting sequence motifs. All seven conserved domains found associated with DNA helicases are present in RAD5. RAD5 also contains a cysteine-rich sequence motif that resembles the corresponding sequences found in 11 other proteins, including those encoded by the DNA repair gene RAD18 and the RAG1 gene required for immunoglobin gene arrangement. A leucine zipper motif preceded by a basic region is also present in RAD5. The cysteine-rich region may coordinate the binding of zinc; this region and the basic segment might constitute distinct DNA-binding domains in RAD5. Possible roles of RAD5 putative ATPase/DNA helicase activity in DNA repair and in the maintenance of wild-type rates of instability of simple repetitive sequences are discussed.

Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum)

Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 436-442 ◽  
Author(s):  
Kurt Weising ◽  
Dieter Kaemmer ◽  
Franz Weigand ◽  
Jörg T. Epplen ◽  
Gunter Kahl
Keyword(s):  
Cicer Arietinum ◽  
Dna Sequences ◽  
Repetitive Sequences ◽  
Banding Patterns ◽  
Simple Repetitive Sequences ◽  
Leguminous Crop ◽  
Oligonucleotide Fingerprinting ◽  
Species Specific ◽  
Synthetic Oligonucleotides ◽  
Different Levels

Synthetic oligonucleotides complementary to simple repetitive DNA sequences were used to detect inter- and intra-specific polymorphisms in a leguminous crop plant (chickpea, Cicer arietinum) and its wild relatives. All the investigated repetitive motifs [(GACA)4, (GATA)4, (GTG)5, (CA)8, (TCC)5, (GGAT)4, and (AGTTT)4] were abundantly present and polymorphic in the chickpea genome. Different probes revealed different levels of variability. Whereas species-specific banding patterns were obtained with the (GTG)5 probe, other probes revealed differences between accessions, or even individuals. The somatic multilocus patterns were stable for all probes.Key words: genetic polymorphism, simple repetitive sequences, DNA fingerprinting, synthetic oligonucleotide probes.

Simple repetitive sequences in the genome: Structure and functional significance

1995 ◽  
Vol 16 (1) ◽  
pp. 1705-1714 ◽  
Author(s):  
Samir K. Brahmachari ◽  
Gopinath Meera ◽  
Partha S. Sarkar ◽  
Pichumani Balagurumoorthy ◽  
Jalaj Tripathi ◽  
...  
Keyword(s):  
Functional Significance ◽  
Repetitive Sequences ◽  
Genome Structure ◽  
Simple Repetitive Sequences

ProbingEphestia DNA for simple repetitive sequences with the digoxigenated oligonucleotide (GTG)5

1991 ◽  
Vol 29 (5-6) ◽  
pp. 287-291 ◽  
Author(s):  
Gerhard M�rsch ◽  
Hannelore Porschke ◽  
Friedrich Leibenguth
Keyword(s):  
Repetitive Sequences ◽  
Simple Repetitive Sequences

Microsatellite fingerprinting in the genus Phaseolus

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 507-515 ◽  
Author(s):  
Andrea Hamann ◽  
Dorothea Zink ◽  
Walter Nagl
Keyword(s):  
Genetic Variability ◽  
Dna Fingerprinting ◽  
Intraspecific Variation ◽  
Repetitive Sequences ◽  
The Other ◽  
Gene Pools ◽  
Oligonucleotide Probes ◽  
Banding Patterns ◽  
Simple Repetitive Sequences ◽  
Almost All

The genetic variability of the genus Phaseolus was investigated by nonradioactive DNA fingerprinting. The simple repetitive sequences (GATA)4, (GACA)4, (CAC)5, and (CA)8 were used as probes to differentiate 18 species comprised of 90 genotypes. (GATA)4, (CAC)5, and (CA)8 could be detected in the genome of nearly all species, while the (GACA)4 motif occurred only in 13 species. Almost all fragments that hybridized with (GACA)4 also hybridized with (GATA)4. All but two cultivars of Phaseolus vulgaris, P. lunatus, P. acutifolius, and P. polyanthus showed specific banding patterns with (GATA)4. The other repetitive motifs revealed only limited or no intraspecific variation. In P. vulgaris, two group-specific patterns were found with (GATA)4, giving further evidence for a Middle American and an Andean origin of the P. vulgaris genotypes. The high intraspecific pattern variation that was revealed with (GATA)4 in the predominantly self-pollinating species P. vulgaris and P. lunatus can probably be explained by there being at least two primary centres of domestication and, hence, genetic diversification. In cross-pollinating species (e.g., P. coccineus), the observed intraspecific variation was, surprisingly, rather low. The present study shows that DNA fingerprinting with microsatellites successfully distinguishes among gene pools, cultivars, and, in some cases, among individuals.Key words: Leguminosae, plants, nonradioactive, simple sequences, digoxigenated oligonucleotide probes.

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