Free-radical oxidation and antiradical defense of the brain during adaptation to chronic stress

1988 ◽  
Vol 106 (2) ◽  
pp. 1091-1094
Author(s):  
N. V. Gulyaeva ◽  
I. P. Levshina
Keyword(s):  
Free Radical ◽  
Chronic Stress ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
The Brain

scholarly journals Influence of neuropeptides acth(4-7)-pro-gly-pro and acth(6-9)-pro-gly-pro on the intensity of redox reactions under experimental depression

2020 ◽  
Vol 10 (3) ◽  
pp. 29-32
Author(s):  
Marina Samotrueva ◽  
Anna Yasenyavskaya ◽  
Aleksandra Tsibizova ◽  
Jumazia Erizhepova ◽  
Nikolai Myasoedov ◽  
...  
Keyword(s):  
Free Radical ◽  
Social Stress ◽  
Redox Reactions ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Initial Content ◽  
The Brain

The experiment is devoted to the study of the antioxidant properties of neuropeptides from melanocortins ACTH(4-7)-Pro-Gly-Pro (Semax) and ACTH(6-9)-ProGly-Pro under conditions of experimental depression. The study was carried out on white outbred male rats. In the process of modeling experimental depression (social stress) inter-male confrontations were observed as a result of which groups of animals with aggressive and submissive behaviors were formed. The free radical oxidation processes were assessed by determining the activity of catalase, the initial content of malondialdehyde (MDA), the rate of spontaneous and ascorbate-dependent lipid peroxidation (LPO) in the hypothalamic and prefrontal regions of the brain by spectrophotometric method. It was found that under the influence of melanocortins, there is a pronounced suppression of the processes of free radical oxidation in the hypothalamic and prefrontal regions of the brain, which arose against the background of a stressful load which is manifested by a decrease in the indicators of the oxidative process.

scholarly journals The effect of thyroxine isomers on the processes of free radical oxidation in the subcellular fractions of the rat cerebral cortex

2000 ◽  
Vol 46 (4) ◽  
pp. 32-34
Author(s):  
O. V. Galkina ◽  
V. M. Prokopenko ◽  
F. E. Putilina ◽  
N. D. Yeshchenko ◽  
A. V. Arutyunyan
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Mitochondrial Fraction ◽  
Rat Cerebral Cortex ◽  
Radical Oxidation ◽  
Adult Rat ◽  
Synaptosomal Fraction ◽  
The Brain

Effective concentrations for D and L thyroxin isomers were determined by the chemiluminescent (CL) method and their effects on free radical oxidation in the mitochondrial and synaptosomal fraction of adult rat cerebral cortex were studied in vitro. A OA DT4 in a model system with riboflavin was 2.2 times higher than L-T4. Effective concentrations for both thyroxin forms were 1$q= = 7.43 x 10~5+/-0.71 M for D-T4 and 15q=15.47 x 10~5+/1.23 M for L-T4. Thyroxin effect on membranous fraction of the brain cortex was studied in vitro using luminol-dependent peroxide CL. In normal concentrations (1 x 108 M) both hormone forms exerted equally intensive antioxidant effect which was more pronounced in the mitochondrial fraction, where CL decreased by 69 and 66%, while in the synaptosomal fraction it decreased only by 45 and 46%. Since D form possesses no hormonal activity, this effect may be due to phenol origin of thyroxin.  

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

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