ACTH, corticosterone, and ?-endorphin in rat blood plasma after prolonged immobilization stress

1985 ◽  
Vol 100 (2) ◽  
pp. 1026-1029 ◽  
Author(s):  
E. A. Kiyatkin ◽  
Yu. V. Polyntsev ◽  
N. E. Kushlinskii ◽  
M. G. Amiragova
Keyword(s):  
Blood Plasma ◽  
Immobilization Stress ◽  
Rat Blood ◽  
Prolonged Immobilization

scholarly journals Analysis of protein profiles in diabetic rat blood plasma that induced by alloxan

2017 ◽  
Author(s):  
Dewi Hidayati ◽  
Nurlita Abdulgani ◽  
Hengki Setiyawan ◽  
Indah Trisnawati ◽  
Nova Maulidina Ashuri ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Diabetic Rat ◽  
Protein Profiles ◽  
Rat Blood

scholarly journals Фотоиндуцированное усиление флуоресценции красителя эванса синего в водном растворе альбумина-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (5) ◽  
pp. 636
Author(s):  
А.А. Намыкин ◽  
А.П. Хороводов ◽  
О.В. Семячкина-Глушковская ◽  
В.В. Тучин ◽  
И.В. Федосов
Keyword(s):  
Blood Plasma ◽  
Evans Blue ◽  
Fluorescence Spectra ◽  
Fluorescence Enhancement ◽  
Albumin Solution ◽  
Enhanced Fluorescence ◽  
Rat Blood ◽  
Dye Fluorescence ◽  
First Time ◽  
In Blood Plasma

AbstractThe effect of photoinduced enhancement of Evans blue (EB) dye fluorescence in blood plasma and albumin solution has been considered. Manifestations of the dye fluorescence enhancement in the albumin solution and rat blood plasma have been compared. Saturation of photoinduced fluorescence, the effect of enhanced fluorescence delay, and divergence in fluorescence spectra of the solutions have been found for the first time. Based on the obtained results, a hypothesis about the presence of nonfluorescent complexes with the EB participation in blood plasma is proposed.

scholarly journals THE CONTENT OF SULFUR-CONTAINING AMINO ACIDS AND RELATED COMPOUNDS IN THE BLOOD PLASMA OF RATS WITH DIFFERENT TYPES OF ALCOHOL INTOXICATION

2021 ◽  
Vol 19 (2) ◽  
pp. 170-175
Author(s):  
A. K. Semenchuk ◽  
◽  
V. V. Lelevich ◽  
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Free Amino ◽  
Alcohol Intoxication ◽  
Chronic Alcohol ◽  
Rat Blood ◽  
Chronic Alcohol Intoxication ◽  
Different Types ◽  
Related Compounds ◽  
Sulfur Containing

Background. Change of the content of sulfur-containing amino acids and their metabolites is one of the pathochemical mechanisms of alcohol intoxication. Purpose of the study. To study the effect of chronic and intermittent alcohol intoxication on the content of sulfurcontaining amino acids and related compounds in the rat blood plasma. Material and methods. Thirty white outbred rats weighing 180-220 g. The content of free amino acids and biogenic amines was determined by HPLC. Results. A 14-day chronic alcohol intoxication was accompanied by a significant decrease in the level of methionine and an increase in the level of homocysteine in the blood plasma. The concentration of glutathione increased by 5%. In the intermittent alcohol intoxication IAI-4 group, the homocysteine content also increased, as did the level of homoserine and cystathionine in the IAI-1 group. Conclusions. Сhronic and intermittent alcohol intoxications cause similar violations of the level of sulfur-containing amino acids and their metabolites in the rat blood plasma.

scholarly journals Activity of endogenous aldehydes catabolism enzymes in subcellular fractions of liver, heart and brain of rats at pubertal age under stress

2012 ◽  
Vol 58 (6) ◽  
pp. 691-701
Author(s):  
L.L. Sukhova ◽  
A.V. Guryeva ◽  
E.A. Berezhnaya ◽  
V.V. Davydov
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Rat Heart ◽  
Immobilization Stress ◽  
Mitochondrial Fraction ◽  
Subcellular Fractions ◽  
Brain Cells ◽  
Redox Transformation ◽  
Effective Utilization ◽  
Old Rats ◽  
Prolonged Immobilization

Activities of enzymes involved in redox transformation of endogenous aldehydes have been investigated in subcellular fractions of liver, heart, and brain of pubertal rats exposed to prolonged immobilization stress. In the liver aldo-keto reductase (AKR) activity in the postmitochondrial fraction and aldehyde dehydrogenase (ALDH) acivity of the mitochondrial fraction demonstrated a more pronounced decrease in 2-month-old rats. Rat heart postmitochondrial AKR and ALDH demonstrated opposite changes in their enzymatic activities, while activity of mitochondrial ALDH remained unchanged. Brain cells create conditions that favor effective utilization of endogenous aldehydes in metabolic redox pathways.

Comments on spectrofluorometric assay of angiotensin-converting enzyme activity in blood plasma of different species

1986 ◽  
Vol 51 (10) ◽  
pp. 2280-2284
Author(s):  
Assia C. Shisheva ◽  
Ognian C. Ikonomov ◽  
Luben M. Sirakov
Keyword(s):  
Blood Plasma ◽  
Hydrolytic Activity ◽  
Converting Enzyme ◽  
Rat Blood ◽  
Spectrofluorometric Determination ◽  
Ace Activity ◽  
Angiotension Converting Enzyme ◽  
Hydrolysis Of ◽  
Rabbit Blood Plasma

The activity of the angiotension -converting enzyme (ACE) in human, dog, rabbit, and rat blood plasma was assayed by spectrofluorometric determination of the product liberated by enzymatic cleavage (L-His-L-Leu). In parallel experiments the hydrolysis of L-His-L-Leu by blood plasma was examined. The hydrolytic activity of rat blood plasma was high and therefore lower values of ACE activity were obtained; the use of the spectrofluorometric assay with rat blood plasma is therefore problematic. By contrast, L-His-L-Leu was not degraded by human, dog, and rabbit blood plasma and the spectrofluorometric determination of this peptide can thus be used to advantage to assay the ACE activity of blood plasma samples of these species.

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