scholarly journals Analysis of protein profiles in diabetic rat blood plasma that induced by alloxan

2017 ◽  
Author(s):  
Dewi Hidayati ◽  
Nurlita Abdulgani ◽  
Hengki Setiyawan ◽  
Indah Trisnawati ◽  
Nova Maulidina Ashuri ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Diabetic Rat ◽  
Protein Profiles ◽  
Rat Blood

Characterization of mercury-binding proteins in rat blood plasma

2018 ◽  
Vol 54 (54) ◽  
pp. 7439-7442 ◽  
Author(s):  
Yiling Li ◽  
Bin He ◽  
Qiying Nong ◽  
Guangbo Qu ◽  
Lihong Liu ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Binding Proteins ◽  
Binding Protein ◽  
Protein Profiles ◽  
Rat Blood

Mercury-binding protein profiles in plasma in vitro and in vivo, which were unclear previously, were systematically investigated.

scholarly journals Фотоиндуцированное усиление флуоресценции красителя эванса синего в водном растворе альбумина-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (5) ◽  
pp. 636
Author(s):  
А.А. Намыкин ◽  
А.П. Хороводов ◽  
О.В. Семячкина-Глушковская ◽  
В.В. Тучин ◽  
И.В. Федосов
Keyword(s):  
Blood Plasma ◽  
Evans Blue ◽  
Fluorescence Spectra ◽  
Fluorescence Enhancement ◽  
Albumin Solution ◽  
Enhanced Fluorescence ◽  
Rat Blood ◽  
Dye Fluorescence ◽  
First Time ◽  
In Blood Plasma

AbstractThe effect of photoinduced enhancement of Evans blue (EB) dye fluorescence in blood plasma and albumin solution has been considered. Manifestations of the dye fluorescence enhancement in the albumin solution and rat blood plasma have been compared. Saturation of photoinduced fluorescence, the effect of enhanced fluorescence delay, and divergence in fluorescence spectra of the solutions have been found for the first time. Based on the obtained results, a hypothesis about the presence of nonfluorescent complexes with the EB participation in blood plasma is proposed.

scholarly journals THE CONTENT OF SULFUR-CONTAINING AMINO ACIDS AND RELATED COMPOUNDS IN THE BLOOD PLASMA OF RATS WITH DIFFERENT TYPES OF ALCOHOL INTOXICATION

2021 ◽  
Vol 19 (2) ◽  
pp. 170-175
Author(s):  
A. K. Semenchuk ◽  
◽  
V. V. Lelevich ◽  
Keyword(s):  
Amino Acids ◽  
Blood Plasma ◽  
Free Amino ◽  
Alcohol Intoxication ◽  
Chronic Alcohol ◽  
Rat Blood ◽  
Chronic Alcohol Intoxication ◽  
Different Types ◽  
Related Compounds ◽  
Sulfur Containing

Background. Change of the content of sulfur-containing amino acids and their metabolites is one of the pathochemical mechanisms of alcohol intoxication. Purpose of the study. To study the effect of chronic and intermittent alcohol intoxication on the content of sulfurcontaining amino acids and related compounds in the rat blood plasma. Material and methods. Thirty white outbred rats weighing 180-220 g. The content of free amino acids and biogenic amines was determined by HPLC. Results. A 14-day chronic alcohol intoxication was accompanied by a significant decrease in the level of methionine and an increase in the level of homocysteine in the blood plasma. The concentration of glutathione increased by 5%. In the intermittent alcohol intoxication IAI-4 group, the homocysteine content also increased, as did the level of homoserine and cystathionine in the IAI-1 group. Conclusions. Сhronic and intermittent alcohol intoxications cause similar violations of the level of sulfur-containing amino acids and their metabolites in the rat blood plasma.

Comments on spectrofluorometric assay of angiotensin-converting enzyme activity in blood plasma of different species

1986 ◽  
Vol 51 (10) ◽  
pp. 2280-2284
Author(s):  
Assia C. Shisheva ◽  
Ognian C. Ikonomov ◽  
Luben M. Sirakov
Keyword(s):  
Blood Plasma ◽  
Hydrolytic Activity ◽  
Converting Enzyme ◽  
Rat Blood ◽  
Spectrofluorometric Determination ◽  
Ace Activity ◽  
Angiotension Converting Enzyme ◽  
Hydrolysis Of ◽  
Rabbit Blood Plasma

The activity of the angiotension -converting enzyme (ACE) in human, dog, rabbit, and rat blood plasma was assayed by spectrofluorometric determination of the product liberated by enzymatic cleavage (L-His-L-Leu). In parallel experiments the hydrolysis of L-His-L-Leu by blood plasma was examined. The hydrolytic activity of rat blood plasma was high and therefore lower values of ACE activity were obtained; the use of the spectrofluorometric assay with rat blood plasma is therefore problematic. By contrast, L-His-L-Leu was not degraded by human, dog, and rabbit blood plasma and the spectrofluorometric determination of this peptide can thus be used to advantage to assay the ACE activity of blood plasma samples of these species.

Improvement of Ketoacidosis in the Diabetic Rat after the Administration of the Oral Antilipolytic Agent Gr 79236

1994 ◽  
Vol 86 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Cecil S. Thompson ◽  
Peter Strong ◽  
Dimitri P. Mikhailidis
Keyword(s):  
Creatine Kinase ◽  
Blood Plasma ◽  
Diabetic Ketoacidosis ◽  
Stomach Contents ◽  
Serum Biochemistry ◽  
Diabetic Rat ◽  
Aspartate Transaminase ◽  
Enhanced Absorption ◽  
Haematological Profile ◽  
Serum Biochemical

1. We assessed the effect of a novel oral antilipolytic agent, N-[(1S, trans) − 2 – hydroxycyclopentyl] adenosine (GR 79236), in experimental diabetic ketoacidosis. Ketotic rats were gavaged with GR 79236 (1 mg/kg) or water (vehicle) and their blood/plasma/serum biochemistry and haematological profile was determined. 2. We found that GR 79236 reduced the plasma non-esterified fatty acid concentration. This effect was associated with the correction of blood/plasma/serum biochemical variables (β-hydroxybutyrate, acetoacetate, triacylglycerol) directly related to diabetic ketoacidosis, and of others (cholesterol, creatinine, creatine kinase and aspartate transaminase) which are not directly related to this metabolic abnormality. 3. There was, however, no evidence of GR 79236 lowering blood glucose in this model. One possible explanation for this observation is that GR 79236 stimulated gastric emptying leading to enhanced absorption of stomach contents when compared with untreated animals.

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