Effect of dibutyryl-cAMP on the content of cholesterol esters in cells of the atherosclerotic human aorta

1983 ◽  
Vol 95 (5) ◽  
pp. 678-680
Author(s):  
V. V. Tertov ◽  
A. N. Orekhov ◽  
V. S. Repin ◽  
V. N. Smirnov
Keyword(s):  
Human Aorta ◽  
Cholesterol Esters ◽  
Dibutyryl Camp ◽  
In Cells

Lipid metabolism in cells of the atherosclerotic human aorta. Experiments in primary culture

1982 ◽  
Vol 94 (4) ◽  
pp. 1410-1412
Author(s):  
V. V. Tertov ◽  
A. N. Orekhov ◽  
V. A. Kosykh ◽  
V. S. Repin
Keyword(s):  
Lipid Metabolism ◽  
Primary Culture ◽  
Human Aorta ◽  
In Cells

Lipids in cells of atherosclerotic and uninvolved human aorta

1991 ◽  
Vol 54 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Dimitry N. Mukhin ◽  
Alexander N. Orekhov ◽  
Elena R. Andreeva ◽  
Eva M. Schindeler ◽  
Vladimir N. Smirnov
Keyword(s):  
Human Aorta ◽  
In Cells

Lipids in cells of atherosclerotic and uninvolved human aorta

1985 ◽  
Vol 43 (2) ◽  
pp. 187-195 ◽  
Author(s):  
A.N. Orekhov ◽  
V.V. Tertov ◽  
V.N. Smirnov
Keyword(s):  
Human Aorta ◽  
In Cells

Position of double bonds in the fatty acids of cholesterol esters from human aorta

1970 ◽  
Vol 12 (1) ◽  
pp. 63-74 ◽  
Author(s):  
J.C. Geer ◽  
R.V. Panganamala ◽  
D.G. Cornwell
Keyword(s):  
Fatty Acids ◽  
Human Aorta ◽  
Cholesterol Esters ◽  
Double Bonds

scholarly journals ACAT Inhibitory Activity of Exudates from Calocedrus Macrolepis Var. Formosana

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701
Author(s):  
Yu-Hsin Hsieh ◽  
Kuan-Jung Chen ◽  
Shih-Chang Chien ◽  
Wen-Ling Cheng ◽  
Jun-Hong Xiao ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Cholesterol Acyltransferase ◽  
Cholesterol Absorption ◽  
Pharmaceutical Product ◽  
Cholesterol Esterification ◽  
Raw 264.7 ◽  
Cholesterol Esters ◽  
Raw 264.7 Cell ◽  
Inhibitory Activities ◽  
In Cells

Cholesterol acyltransferase (ACAT) is an enzyme controlling cholesterol esterification in cells. Large amounts of cholesterol esters accumulate in macrophages and smooth muscle cells of blood vessel walls resulting in the initial stages of atherosclerosis. Thus, atherosclerosis might be inhibited through inhibition of the activity of ACAT. In the present study, we identified by spectral analysis and chromatographic quantification that ferruginol was the most abundant component of exudates secreted from the wounding site of Calocedrus macrolepis Kurz var. formosana. Results obtained from the cholesterol absorption assay revealed that ferruginol exhibited a significant inhibitory activity on cholesterol absorption in mice macrophages (RAW 264.7 cell). Based on the results from analyzing the ratio of cholesterol esterification, ferruginol dose-dependently suppressed cholesterol esterification and the IC50 value was 2.0 μg/mL. In conclusion, ferruginol revealed strong inhibitory activities that retarded the absorption and esterification of cholesterol in cells. Our finding indicates that ferruginol might possess a potential for development as a pharmaceutical product for preventing arteriosclerosis.

Signal Transduction Pathways Underlying the Expression of Tissue Factor and Thrombomodulin in Promyelocytic Cells Induced to Differentiate by Retinoid Acid and Dibutyryl cAMP

2001 ◽  
Vol 85 (06) ◽  
pp. 1031-1036 ◽  
Author(s):  
Pablo Dobado-Berrios ◽  
Rosa Ros ◽  
Antonio Torres ◽  
Socorro García-Navarro ◽  
Mercé Jardí ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Tissue Factor ◽  
Inositol Phosphate ◽  
Signal Transduction Pathway ◽  
Dibutyryl Camp ◽  
Opposite Pattern ◽  
Cd11b Expression ◽  
Retinoid Acid ◽  
Inositol Uptake ◽  
In Cells

SummaryAcute promyelocytic leukaemia (APL) may be associated with disseminated intravascular coagulation, as a result of increased tissue factor (TF) expression and reduced thrombomodulin (TM) expression by APL blast cells. During retinoid acid (RA)- and dibutyryl cAMP (dbcAMP)-induced differentiation of the APL cells, there is a marked up-modulation of both the protein kinase A (PKA) and C (PKC) activities. In order to further assess whether these kinases are intimately associated with both the differentiation process and the regulation of TF and TM expression, we have correlated the modulation of their respective pathways with the extent of differentiation and modulation of these cellular receptors. NB4 cells were incubated with all-trans-RA (ATRA) or dbcAMP for up to 48 h. The contribution of phospholipase C (PLC), inositol phosphate (IP), PKC and PKA in the expression of CD11b, TF and TM was studied by the use of specific inhibitors. Myo-inositol uptake and PKC activity increased in cells induced to differentiate by ATRA but the retinoid did not affect cAMP levels or PKA activity. Under treatment with dbcAMP, PKA activity was increased while inositol uptake and PKC activity remained unchanged. Our results show that the effects of ATRA and dbcAMP on promyelocytic cells are closely related, respectively, to the PLC/IP/PKC and the cAMP/PKA pathways. In cells induced to differentiate by ATRA, CD11b expression seems more closely related to inositol uptake than to PKC activity while the expression of TF and TM show the opposite pattern, which suggests cellular events regulated at a different level within a common signal transduction pathway.

Lipids in cells of atherosclerotic and uninvolved human aorta

1985 ◽  
Vol 42 (1) ◽  
pp. 117-137 ◽  
Author(s):  
A.N Orekhov ◽  
V.V Tertov ◽  
I.D Novikov ◽  
A.V Krushinsky ◽  
E.R Andreeva ◽  
...  
Keyword(s):  
Human Aorta ◽  
In Cells

Cytomegalovirus genome and the immediate-early antigen in cells of different layers of human aorta

2000 ◽  
Vol 436 (6) ◽  
pp. 539-552 ◽  
Author(s):  
Sergey Yu. Pampou ◽  
Sergey N. Gnedoy ◽  
V. B. Bystrevskaya ◽  
Vladimir N. Smirnov ◽  
Eugene I. Chazov ◽  
...  
Keyword(s):  
Immediate Early ◽  
Human Aorta ◽  
Early Antigen ◽  
Immediate Early Antigen ◽  
In Cells
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