Lipid metabolism in cells of the atherosclerotic human aorta. Experiments in primary culture

1982 ◽  
Vol 94 (4) ◽  
pp. 1410-1412
Author(s):  
V. V. Tertov ◽  
A. N. Orekhov ◽  
V. A. Kosykh ◽  
V. S. Repin
Keyword(s):  
Lipid Metabolism ◽  
Primary Culture ◽  
Human Aorta ◽  
In Cells

Control of Calcium Influx in Cells Without Action Potentials

Physiology ◽  
1988 ◽  
Vol 3 (6) ◽  
pp. 244-249
Author(s):  
DV Gallacher
Keyword(s):  
Lipid Metabolism ◽  
Cell Surface ◽  
Action Potentials ◽  
Calcium Influx ◽  
Inositol Polyphosphate ◽  
Intracellular Fluid ◽  
Common Effect ◽  
Inositol Lipids ◽  
Intracellular Organelles ◽  
In Cells

A common effect of neurotransmitters and hormones that stimulate the metabolism of inositol lipids is the ability to increase the permeability of membranes to Ca2+. This effect occurs at surface membranes by the influx of Ca2+ from extracellular to intracellular fluid and at internal membranes by release of Ca2+ sequestered in intracellular organelles. Recent evidence suggests that it is the inositol polyphosphate products of lipid metabolism that regulate Ca2+ fluxes across both internal and cell surface membranes.

Effect of dibutyryl-cAMP on the content of cholesterol esters in cells of the atherosclerotic human aorta

1983 ◽  
Vol 95 (5) ◽  
pp. 678-680
Author(s):  
V. V. Tertov ◽  
A. N. Orekhov ◽  
V. S. Repin ◽  
V. N. Smirnov
Keyword(s):  
Human Aorta ◽  
Cholesterol Esters ◽  
Dibutyryl Camp ◽  
In Cells

Expression of fatty-acid-binding proteins in cells involved in lung-specific lipid metabolism

1998 ◽  
Vol 253 (2) ◽  
pp. 430-436 ◽  
Author(s):  
Florian Guthmann ◽  
Carsten Hohoff ◽  
Henry Fechner ◽  
Peter Humbert ◽  
Torsten Borchers ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Specific Lipid ◽  
In Cells

scholarly journals Nonsense suppressor therapies rescue peroxisome lipid metabolism and assembly in cells from patients with specific PEX gene mutations

2011 ◽  
Vol 112 (5) ◽  
pp. 1250-1258 ◽  
Author(s):  
Patricia K. Dranchak ◽  
Erminia Di Pietro ◽  
Ann Snowden ◽  
Nathan Oesch ◽  
Nancy E. Braverman ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Gene Mutations ◽  
Nonsense Suppressor ◽  
In Cells

Endurance training alters outward K+current characteristics in rat cardiocytes

2001 ◽  
Vol 90 (4) ◽  
pp. 1327-1333 ◽  
Author(s):  
Korinne N. Jew ◽  
M. Charlotte Olsson ◽  
Eric A. Mokelke ◽  
Bradley M. Palmer ◽  
Russell L. Moore
Keyword(s):  
Membrane Potential ◽  
Action Potential ◽  
Primary Culture ◽  
Left Ventricular ◽  
Resting Membrane Potential ◽  
Sprague Dawley ◽  
Whole Cell ◽  
Time To Peak ◽  
Time Required ◽  
In Cells

The effect of endurance run training on outward K+ currents with rapidly inactivating ( I to) and sustained or slowly inactivating ( I sus) characteristics was examined in left ventricular (LV) cardiocytes isolated from sedentary (Sed) and treadmill-trained (Tr) female Sprague-Dawley rats. Isolated LV cardiocytes were used in whole cell patch-clamp studies to characterize whole cell I to and I sus. Peak I to was greatest in cells isolated from the Tr group. When I to was corrected for cell capacitance to yield a current density, most, but not all, of the Sed vs. Tr differences in I to magnitude were eliminated. Regardless of how I to was expressed (e.g., I to or I todensity), the time required to achieve a peak value was markedly shortened in the cardiocytes isolated from the Tr group. Training elicited a reduction in I sus density. Action potential characteristics were determined in Sed and Tr cardiocytes in primary culture. Training did not affect resting membrane potential, whereas peak membrane potential was reduced and time to peak membrane potential was prolonged in the Tr group. In addition, time to 50% repolarization was significantly increased in cells from the Tr group. Collectively, these data indicate that I to and I sus characteristics are altered by training in isolated LV cardiocytes. These alterations in I to and I sus may be responsible, at least in part, for the training-induced alterations in action potential configuration in cardiocytes in primary culture.

scholarly journals Telomerase activity in primary cultures of normal adrenocortical cells

2001 ◽  
Vol 170 (3) ◽  
pp. 677-684 ◽  
Author(s):  
T Suwa ◽  
L Yang ◽  
PJ Hornsby
Keyword(s):  
Primary Culture ◽  
Adrenal Cortex ◽  
Cell Cultures ◽  
Primary Cultures ◽  
Telomerase Activity ◽  
Adrenocortical Cell ◽  
Isolated Cells ◽  
Adrenocortical Cells ◽  
Human Telomerase Reverse Transcriptase ◽  
In Cells

Telomerase activity was measured in isolated cells from bovine and human adrenal cortex, in cells in primary culture, in cells in later passages in culture, and in cells genetically modified by expression of hTERT (human telomerase reverse transcriptase). Telomerase activity in freshly isolated bovine adrenocortical cells and in human adrenal cells from donors of various ages (6-79 years) was very low or undetected. However, primary bovine adrenocortical cell cultures were strongly positive for telomerase activity, and primary human adrenocortical cell cultures were weakly positive. Both cell types proliferate in primary culture but proliferation of bovine cells is much more vigorous. When primary bovine cells were subcultured to make successively secondary and tertiary cultures, telomerase activity declined strongly, and was undetected by the third passage. There was only a slight decrease in growth rate over this period. Levels of the telomerase RNA component did not change with passage number when assessed by semi-quantitative competitive RT-PCR. When both bovine and human cells were infected with a retrovirus encoding hTERT, telomerase activity in the cells was very high. We conclude that in the adrenal cortex, as in some other tissues, TERT expression is regulated and upregulation of telomerase activity is associated with rapid proliferation in primary culture. Telomerase activity is not maintained, and introduction of TERT is required for stable telomerase activity and for immortalization.

scholarly journals Leptin Is an Important Endocrine Player That Directly Activates Gonadotropic Cells in Teleost Fish, Chub Mackerel

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3505
Author(s):  
Hirofumi Ohga ◽  
Kosuke Ito ◽  
Kohei Kakino ◽  
Hiroaki Mon ◽  
Takahiro Kusakabe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Primary Culture ◽  
Teleost Fish ◽  
Pituitary Cells ◽  
Chub Mackerel ◽  
Gonadotropic Cells ◽  
B Subunit ◽  
Silkworm Pupae ◽  
Males And Females ◽  
In Cells

Leptin, secreted by adipocytes, directly influences the onset of puberty in mammals. Our previous study showed that leptin stimulation could promote the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from pituitary cells in primary culture and ovarian development in chub mackerel. This study aimed to elucidate the detailed mechanism of leptin-induced effects on gonadotropin hormone-producing cells. We produced recombinant leptin using silkworm pupae and investigated the effects of leptin on FSH and LH secretion and gene expression in the primary culture of pituitary cells from chub mackerel. The presence or absence of co-expression of lepr mRNA, FSH and LH b-subunit mRNA in gonadotropic cells was examined by double-labeled in situ hybridization. The addition of leptin significantly increased the secretion and gene expression of FSH and LH from male and female pituitary cells in primary culture. In contrast, gonadotropin-releasing hormone 1 affected neither FSH secretion in cells from females nor fshb and lhb expression in cells from males and females. The expression of lepr was observed in FSH- and LH-producing cells of both males and females. The results indicate that leptin directly regulates gonadotropin synthesis and secretion and plays an important role in the induction of puberty in teleost fish.

Lipid metabolism in cells grown in tissue culture: O-alkyl, O-alk-1-enyl, and acyl moieties of L-M cells

1969 ◽  
Vol 176 (3) ◽  
pp. 491-501 ◽  
Author(s):  
Robert E. Anderson ◽  
Robert B. Cumming ◽  
Marva Walton ◽  
Fred Snyder
Keyword(s):  
Tissue Culture ◽  
Lipid Metabolism ◽  
M Cells ◽  
In Cells
Sign in / Sign up

Export Citation Format

Share Document