High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements

1994 ◽  
Vol 14 (3) ◽  
pp. 183-190 ◽  
Author(s):  
Yun-Seung Kyung ◽  
Madhusudan V. Peshwa ◽  
David M. Gryte ◽  
Wei-Shou Hu
Keyword(s):  
Oxygen Uptake ◽  
Uptake Rate ◽  
Mammalian Cells ◽  
Oxygen Uptake Rate ◽  
High Density ◽  
High Density Culture ◽  
On Line ◽  
Line Oxygen

New Control Parameters and Measurement Techniques for the Activated Sludge Process

1993 ◽  
Vol 27 (5-6) ◽  
pp. 287-295 ◽  
Author(s):  
M. Pelkonen ◽  
R. Tenno
Keyword(s):  
Oxygen Uptake ◽  
Production Rate ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
Measurement Techniques ◽  
Co2 Production ◽  
Carbon Oxidation ◽  
Nitrification Process ◽  
On Line ◽  
Line Oxygen

An on-line oxygen uptake and CO2-production rate measurement system was used to analyse the biomass activity. CO2-production rate corresponded well with oxygen uptake rate in the carbon oxidation process. In the nitrification process the ratio CO2-prod./O2-consumed (rq) had a larger variation, but CO2-production had a good correlation with utilized COD; variation of the rq-value indicate differences in the auto-/heterotrop-hic oxidation balarice. From this basis the oxygen uptake rate for carbon oxidation and nitrification can be separated. The comparison with two biomass determination methods based on ATP-analysis shows that for heterotrophic biomass the methods indicate clearly different changes of ATP, for nitrification process the results were more compatible. By combining the biomass and on-line measurements with biomass growth model based on IAWPRC-model equations reasonably good estimates were obtained. Best fit of model was in the nitrification process; by using VSS as the measure of biomass the fit was poor. The differences in the ATP-measurement results affect also the model coefficients.

Oxygen Transfer Efficiency Measurements Using Off-Gas Techniques

1989 ◽  
Vol 21 (10-11) ◽  
pp. 1295-1300 ◽  
Author(s):  
W. C. Boyle ◽  
B. G. Hellstrom ◽  
L. Ewing
Keyword(s):  
Oxygen Uptake ◽  
Uptake Rate ◽  
Oxygen Transfer ◽  
Oxygen Uptake Rate ◽  
Transfer Efficiency ◽  
Theoretical Development ◽  
Process Conditions ◽  
Uptake Rates ◽  
Oxygen Transfer Efficiency ◽  
On Line

The off-gas technique for measuring oxygen transfer efficiency in aeration tanks under process conditions was proposed as an accurate technique for calibrating or verifying on-line methods used to estimate oxygen uptake rates. The theoretical development of the off-gas technique was presented. Application of this technique in verifying or calibrating existing on-line methods for estimating oxygen uptake rate was presented. Direct application as an on-line method for estimating oxygen uptake rate was also discussed.

Oxygen Uptake Rate (OUR) Estimation in High Density Mammalian Cell Perfusion Cultures

2010 ◽  
pp. 837-841 ◽  
Author(s):  
C. Cruz ◽  
K. Joeris ◽  
C. Goudar ◽  
C. Zhang ◽  
K. Konstantinov
Keyword(s):  
Oxygen Uptake ◽  
Mammalian Cell ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
High Density ◽  
Perfusion Cultures

A new method for on-line measurement of the volumetric oxygen uptake rate in membrane aerated animal cell cultures

2000 ◽  
Vol 78 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Paul Ducommun ◽  
Pierre-Alain Ruffieux ◽  
Maria-Pilar Furter ◽  
Ian Marison ◽  
Urs von Stockar
Keyword(s):  
Oxygen Uptake ◽  
Cell Cultures ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
Animal Cell ◽  
New Method ◽  
Line Measurement ◽  
Animal Cell Cultures ◽  
On Line
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