On-line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine

1995 ◽  
Vol 45 (1) ◽  
pp. 54-62 ◽  
Author(s):  
K. Eyer ◽  
A. Oeggerli ◽  
E. Heinzle
Keyword(s):  
Oxygen Uptake ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
Animal Cell ◽  
Gas Analysis ◽  
Cell Cultivation ◽  
Rate Estimation ◽  
On Line

A new method for on-line measurement of the volumetric oxygen uptake rate in membrane aerated animal cell cultures

2000 ◽  
Vol 78 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Paul Ducommun ◽  
Pierre-Alain Ruffieux ◽  
Maria-Pilar Furter ◽  
Ian Marison ◽  
Urs von Stockar
Keyword(s):  
Oxygen Uptake ◽  
Cell Cultures ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
Animal Cell ◽  
New Method ◽  
Line Measurement ◽  
Animal Cell Cultures ◽  
On Line

Mass transfer impacts in flocculent and granular biomass from SBR systems

2004 ◽  
Vol 50 (10) ◽  
pp. 203-212 ◽  
Author(s):  
D. Gapes ◽  
B.-M. Wilén ◽  
J. Keller
Keyword(s):  
Mass Transfer ◽  
Particle Size ◽  
Oxygen Uptake ◽  
Activated Sludge ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
Batch Reactor ◽  
Average Particle Size ◽  
Gas Analysis ◽  
Average Particle

An experimental study was conducted to describe mass transfer impacts within nitrifying aggregates sourced from sequencing batch reactor (SBR) activated sludge systems. Flocculent and granular sludge with high nitrification activity was obtained in two laboratory SBR systems, supplied with a synthetic, ammonium-based feed. The flocculent biomass was fractionated using a sieving procedure, in order to obtain biomass fractions with different particle size distributions. The oxygen uptake rate (OUR) response to changes in dissolved oxygen concentration was measured under highly controlled conditions in a titrimetric and off-gas analysis (TOGA) sensor, and the results used to assess mass transfer effects. As the average particle size of the biomass increased, mass transfer limitations were found to increase significantly. Empirically fitted, apparent KS,O2 values were demonstrated to be highly dependent on particle size, and reflect the mass transfer limitations occurring in the aggregates within a given system. Such parameters thus have little to do with the actual biokinetic parameter from which they are derived. The results obtained from the TOGA sensor study were consistent with those obtained from a microelectrode study on the same nitrifying granules. Together, these studies add considerable weight to the conclusion that consideration of external and internal mass transfer limitations is vital to the accurate description of activated sludge treatment processes, particularly those with a high oxygen uptake rate.

Oxygen Transfer Efficiency Measurements Using Off-Gas Techniques

1989 ◽  
Vol 21 (10-11) ◽  
pp. 1295-1300 ◽  
Author(s):  
W. C. Boyle ◽  
B. G. Hellstrom ◽  
L. Ewing
Keyword(s):  
Oxygen Uptake ◽  
Uptake Rate ◽  
Oxygen Transfer ◽  
Oxygen Uptake Rate ◽  
Transfer Efficiency ◽  
Theoretical Development ◽  
Process Conditions ◽  
Uptake Rates ◽  
Oxygen Transfer Efficiency ◽  
On Line

The off-gas technique for measuring oxygen transfer efficiency in aeration tanks under process conditions was proposed as an accurate technique for calibrating or verifying on-line methods used to estimate oxygen uptake rates. The theoretical development of the off-gas technique was presented. Application of this technique in verifying or calibrating existing on-line methods for estimating oxygen uptake rate was presented. Direct application as an on-line method for estimating oxygen uptake rate was also discussed.

High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements

1994 ◽  
Vol 14 (3) ◽  
pp. 183-190 ◽  
Author(s):  
Yun-Seung Kyung ◽  
Madhusudan V. Peshwa ◽  
David M. Gryte ◽  
Wei-Shou Hu
Keyword(s):  
Oxygen Uptake ◽  
Uptake Rate ◽  
Mammalian Cells ◽  
Oxygen Uptake Rate ◽  
High Density ◽  
High Density Culture ◽  
On Line ◽  
Line Oxygen

Dissolved Oxygen Control Based on Real Time Oxygen Uptake Rate Estimation

2010 ◽  
Vol 2010 (8) ◽  
pp. 7400-7408 ◽  
Author(s):  
Gregory Duffy ◽  
Steven Kestel ◽  
Matthew Gray ◽  
George Lee ◽  
Tilo Stahl ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Dissolved Oxygen ◽  
Real Time ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
Oxygen Control ◽  
Rate Estimation ◽  
Dissolved Oxygen Control
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