Ultrastructural study of the effects of coral skeleton on cultured human gingival fibroblasts in three-dimensional collagen lattices

1991 ◽  
Vol 2 (3) ◽  
pp. 162-167 ◽  
Author(s):  
A. H. M. Shabana ◽  
J. P. Ouhayoun ◽  
H. Boulekbache ◽  
J. M. Sautier ◽  
N. Forest
Keyword(s):  
Ultrastructural Study ◽  
Three Dimensional ◽  
Coral Skeleton ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Collagen Lattices

Cell-matrix and cell-cell interactions of human gingival fibroblasts on three-dimensional nanofibrous gelatin scaffolds

2012 ◽  
Vol 8 (11) ◽  
pp. 862-873 ◽  
Author(s):  
Ashneet Sachar ◽  
T. Amanda Strom ◽  
Symone San Miguel ◽  
Maria J. Serrano ◽  
Kathy K. H. Svoboda ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Interactions ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cell Matrix ◽  
Cell Cell

scholarly journals Human Gingival Fibroblasts Display a Non-Fibrotic Phenotype Distinct from Skin Fibroblasts in Three-Dimensional Cultures

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90715 ◽  
Author(s):  
Wesley Mah ◽  
Guoqiao Jiang ◽  
Dylan Olver ◽  
Godwin Cheung ◽  
Ben Kim ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Skin Fibroblasts ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

Effects of Manufacturing and Finishing Techniques of Feldspathic Ceramics on Surface Topography, Biofilm Formation, and Cell Viability for Human Gingival Fibroblasts

2018 ◽  
Vol 43 (6) ◽  
pp. 593-601 ◽  
Author(s):  
LPC Contreras ◽  
AMO Dal Piva ◽  
FC Ribeiro ◽  
LC Anami ◽  
SEA Camargo ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Computer Aided Design ◽  
Three Dimensional ◽  
Human Gingival Fibroblasts ◽  
Cytotoxicity Test ◽  
Gingival Fibroblasts ◽  
Computer Aided ◽  
Cad Cam ◽  
Diphenyl Tetrazolium Bromide ◽  
Ceramic Restorations

SUMMARY Purpose: Feldspathic ceramic restorations can be obtained by different techniques (stratification or computer-aided design/computer-aided manufacturing [CAD/CAM] blocks) and finishing procedures (polishing or glaze application). This study evaluated the effects of techniques and finishing procedures on surface properties, biofilm formation, and viability of human gingival fibroblasts (FMM-1) in contact with these materials. Methods and Materials: Ceramic specimens were obtained through a stratification technique (Vita VM9) and from CAD/CAM blocks (Vita Blocs Mark II; both Vita Zahnfabrik) and their surfaces were finished by polishing (ceramisté diamond rubbers + polishing paste; “p” subgroups) or glaze spray application + sintering (“g” subgroups). Roughness (Ra and RSm parameters) and surface free energy (SFE) were measured. Early biofilm formation of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans was evaluated by counting colony-forming units (CFU). MTT (3-[4.5-dimethyl-thiazol-2-yl-]-2.5-diphenyl tetrazolium bromide) cytotoxicity test evaluated cellular viability for the growth of FMM-1 after 24 hours and seven days of contact. Scanning electron microscopy (SEM) and three-dimensional optical profilometry were performed to qualitatively analyze the surface. Data were analyzed by analysis of variance, Tukey test, and t-test (all α=0.05). Results: Polished samples presented lower roughness (Ra, p=0.015; RSm, p=0.049) and higher SFE (p=0.00). Streptococci had higher CFU in all groups, but the CFU of C albicans was lower for polished samples. Biofilm formation was influenced by the interaction of all factors (p=0.018), and the materials showed no cytotoxicity to FMM-1 growth. Conclusions: Polishing resulted in the lowest values for surface roughness and higher SFE values. Polished ceramics showed less C albicans adherence while the adherence of Streptococci was greater than C albicans in all conditions.

A Preliminary Study of the In Vitro Biocompatibility Testing of Silk Fibroin/Alpha Tricalcium Phosphate Composite Scaffolds for Bone Tissue Engineering

2017 ◽  
Vol 751 ◽  
pp. 605-610
Author(s):  
Woradej Pichaiaukrit ◽  
Wiriya Juwattanasamran ◽  
Sorada Kanokpanont
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Dna Assays ◽  
Contrast Microscopy

Silk fibroin is a natural biodegradable polymer that has been demonstrated for use as scaffolds for bone tissue engineering. To improve the osteoconductivity and the osteoinductivity of silk fibroin scaffolds, ceramics were added. α-tricalcium phosphate (α-TCP) is the expected ceramic that useful for scaffolds for bone tissue engineering either alone or blended with silk fibroin. From the previous study, we evaluated the mechanical properties of three-dimensional porous silk fibroin/ α-TCP scaffolds and concluded that the scaffolds containing 8% (w/w) α-TCP exhibited the highest compressive modulus. The objective of this study was to evaluate the biological properties of three-dimensional porous silk fibroin/α-TCP scaffolds. The scaffolds were constructed using a solvent casting and salt leaching technique. The hybrid strain of degummed Thai silk fibroin, Nangnoi Srisaket 1 x Mor, was dissolved in hexafluoroisopropanol at 16% (w/v). α-TCP was incorporated to produce 4, 8, 12, and 16 wt% solution. Sucrose (particle size 250-450 μm; sucrose/silk fibroin = 8.5/1 w/w) was used as a porogen. Human gingival fibroblasts (passage 5) were cultured in these scaffolds. After 72 h, the biocompatibility of seeded scaffolds was evaluated under the inverted phase contrast microscopy. Cell proliferation was determined by DNA assays and scanning electron microscopy. The images from inverted phase contrast microscopy revealed the human gingival fibroblasts can be attached at the surface of scaffolds in all groups. The results from the DNA assays showed that the number of human gingival fibroblasts was increased as the culture period was prolonged but was not as the increasing of α-TCP. At 120 h, the scaffolds containing 8% (w/w) α-TCP exhibited the highest cell number. The scanning electron microscope images at 24, 72, and 120 h after cell culturing presented human gingival fibroblasts can be expanded well and exhibited the normal morphology. The results suggested that the scaffolds containing 8% (w/w) α-TCP may be a potential candidate for bone tissue engineering applications.

scholarly journals Ultrastructural Study of the Fibrogenesis of Elastic System Fibers by Human Gingival Fibroblasts in vitro.

1999 ◽  
Vol 41 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Takaaki Kunimoto ◽  
Shigehito Fujii ◽  
Kazuharu Irie ◽  
Yasunori Sakakura ◽  
Toshihiko Yajima
Keyword(s):  
Ultrastructural Study ◽  
Elastic System ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

Matrix expression and proliferation of primary gingival fibroblasts in a three-dimensional cell culture model

1999 ◽  
Vol 112 (17) ◽  
pp. 2823-2832 ◽  
Author(s):  
G. Hillmann ◽  
A. Gebert ◽  
W. Geurtsen
Keyword(s):  
Extracellular Matrix ◽  
Culture System ◽  
Cultured Cells ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Human Gingival Fibroblasts ◽  
Confocal Laser ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Histological Techniques

The growth of cultured primary human gingival fibroblasts and the three-dimensional arrangement of the extracellular matrix in a polyester carrier system was investigated using various histological techniques. The results were compared with monolayer cultures. Collagen types I, III, V, and VI were investigated by conventional and fluorescence microscopy, scanning and transmission electron microscopy, and confocal laser scanning microscopy. Human gingival fibroblasts were obtained from tissue biopsies of five donors and were cultivated up to 5 weeks under three-dimensional culture conditions. The cells displayed an elongated, spindle-like or stellate morphology resembling the in vivo situation. Collagen type I revealed thick fiber bundles, and collagens type III and V were distributed as fine fibrils or small bundles throughout the culture system. Frequently, the fibers were oriented parallel to the long axis of the cells. Type VI collagen formed thin fibers and revealed a reticular pattern. In histological sections the cultured cells exhibited a morphology clearly different from that of cells cultured in monolayers. Their shape and spatial distribution resembled that of cells in tissue biopsies more closely. The culture system presented here promotes a dynamic model for performing studies for instance on the interactions of cultured cells with extracellular matrix molecules, on the pathogenesis of inflammatory processes or on the interactions with biomaterials, thus providing qualitative and quantitative information.

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