Cell-matrix and cell-cell interactions of human gingival fibroblasts on three-dimensional nanofibrous gelatin scaffolds

2012 ◽  
Vol 8 (11) ◽  
pp. 862-873 ◽  
Author(s):  
Ashneet Sachar ◽  
T. Amanda Strom ◽  
Symone San Miguel ◽  
Maria J. Serrano ◽  
Kathy K. H. Svoboda ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Cell Interactions ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cell Matrix ◽  
Cell Cell

3230 Cell-Matrix and Cell-Cell Interactions and the Aqueous Humor Outflow In Trabecular Meshwork (TM) Cells

SciVee ◽  
2012 ◽  
Author(s):  
Xinbo Li ◽  
Diala Abu-Hassan ◽  
Janice Vranka ◽  
John Bradley ◽  
Ted Acott ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Trabecular Meshwork ◽  
Cell Interactions ◽  
Aqueous Humor Outflow ◽  
Cell Matrix ◽  
Cell Cell

scholarly journals Physical confinement signals regulate the organization of stem cells in three dimensions

2016 ◽  
Vol 13 (123) ◽  
pp. 20160613 ◽  
Author(s):  
Sebastian V. Hadjiantoniou ◽  
David Sean ◽  
Maxime Ignacio ◽  
Michel Godin ◽  
Gary W. Slater ◽  
...  
Keyword(s):  
Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Cell Interactions ◽  
Three Dimensions ◽  
Cell Substrate ◽  
Cell Cell

During embryogenesis, the spherical inner cell mass (ICM) proliferates in the confined environment of a blastocyst. Embryonic stem cells (ESCs) are derived from the ICM, and mimicking embryogenesis in vitro , mouse ESCs (mESCs) are often cultured in hanging droplets. This promotes the formation of a spheroid as the cells sediment and aggregate owing to increased physical confinement and cell–cell interactions. In contrast, mESCs form two-dimensional monolayers on flat substrates and it remains unclear if the difference in organization is owing to a lack of physical confinement or increased cell–substrate versus cell–cell interactions. Employing microfabricated substrates, we demonstrate that a single geometric degree of physical confinement on a surface can also initiate spherogenesis. Experiment and computation reveal that a balance between cell–cell and cell–substrate interactions finely controls the morphology and organization of mESC aggregates. Physical confinement is thus an important regulatory cue in the three-dimensional organization and morphogenesis of developing cells.

scholarly journals Computational modelling of epidermal stratification highlights the importance of asymmetric cell division for predictable and robust layer formation

2014 ◽  
Vol 11 (99) ◽  
pp. 20140631 ◽  
Author(s):  
Alexander Gord ◽  
William R. Holmes ◽  
Xing Dai ◽  
Qing Nie
Keyword(s):  
Cell Adhesion ◽  
Cell Division ◽  
Asymmetric Cell Division ◽  
Three Dimensional ◽  
Interaction Strength ◽  
Computational Modelling ◽  
Protective Layer ◽  
The Body ◽  
Cell Interactions ◽  
Cell Cell

Skin is a complex organ tasked with, among other functions, protecting the body from the outside world. Its outermost protective layer, the epidermis, is comprised of multiple cell layers that are derived from a single-layered ectoderm during development. Using a new stochastic, multi-scale computational modelling framework, the anisotropic subcellular element method, we investigate the role of cell morphology and biophysical cell–cell interactions in the formation of this layered structure. This three-dimensional framework describes interactions between collections of hundreds to thousands of cells and (i) accounts for intracellular structure and morphology, (ii) easily incorporates complex cell–cell interactions and (iii) can be efficiently implemented on parallel architectures. We use this approach to construct a model of the developing epidermis that accounts for the internal polarity of ectodermal cells and their columnar morphology. Using this model, we show that cell detachment, which has been previously suggested to have a role in this process, leads to unpredictable, randomized stratification and that this cannot be abrogated by adjustment of cell–cell adhesion interaction strength. Polarized distribution of cell adhesion proteins, motivated by epithelial polarization, can however eliminate this detachment, and in conjunction with asymmetric cell division lead to robust and predictable development.

scholarly journals Human Gingival Fibroblasts Display a Non-Fibrotic Phenotype Distinct from Skin Fibroblasts in Three-Dimensional Cultures

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90715 ◽  
Author(s):  
Wesley Mah ◽  
Guoqiao Jiang ◽  
Dylan Olver ◽  
Godwin Cheung ◽  
Ben Kim ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Skin Fibroblasts ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

scholarly journals Reversible Disruption of Cell-Matrix and Cell-Cell Interactions by Overexpression of Sialomucin Complex

1997 ◽  
Vol 272 (52) ◽  
pp. 33245-33254 ◽  
Author(s):  
Masanobu Komatsu ◽  
Coralie A. Carothers Carraway ◽  
Nevis L. Fregien ◽  
Kermit L. Carraway
Keyword(s):  
Cell Interactions ◽  
Cell Matrix ◽  
Sialomucin Complex ◽  
Cell Cell
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