collagen lattices
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Cells ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 128
Author(s):  
Adam Boros-Rausch ◽  
Oksana Shynlova ◽  
Stephen James Lye

Prophylactic administration of the broad-spectrum chemokine inhibitor (BSCI) FX125L has been shown to suppress uterine contraction, prevent preterm birth (PTB) induced by Group B Streptococcus in nonhuman primates, and inhibit uterine cytokine/chemokine expression in a murine model of bacterial endotoxin (LPS)-induced PTB. This study aimed to determine the mechanism(s) of BSCI action on human myometrial smooth muscle cells. We hypothesized that BSCI prevents infection-induced contraction of uterine myocytes by inhibiting the secretion of pro-inflammatory cytokines, the expression of contraction-associated proteins and disruption of myocyte interaction with tissue macrophages. Myometrial biopsies and peripheral blood were collected from women at term (not in labour) undergoing an elective caesarean section. Myocytes were isolated and treated with LPS with/out BSCI; conditioned media was collected; cytokine secretion was analyzed by ELISA; and protein expression was detected by immunoblotting and immunocytochemistry. Functional gap junction formation was assessed by parachute assay. Collagen lattices were used to examine myocyte contraction with/out blood-derived macrophages and BSCI. We found that BSCI inhibited (1) LPS-induced activation of transcription factor NF-kB; (2) secretion of chemokines (MCP-1/CCL2 and IL-8/CXCL8); (3) Connexin43-mediated intercellular connectivity, thereby preventing myocyte–macrophage crosstalk; and (4) myocyte contraction. BSCI represents novel therapeutics for prevention of inflammation-induced PTB in women.


Author(s):  
Mario Chopin-Doroteo ◽  
Rosa M. Salgado-Curiel ◽  
José Pérez-González ◽  
Benjamín M. Marín-Santibáñez ◽  
Edgar Krötzsch

2017 ◽  
Vol 16 (5) ◽  
pp. 1743-1763 ◽  
Author(s):  
Shakti N. Menon ◽  
Cameron L. Hall ◽  
Scott W. McCue ◽  
D. L. Sean McElwain

2013 ◽  
Vol 201 (7) ◽  
pp. 1069-1084 ◽  
Author(s):  
Katarina Wolf ◽  
Mariska te Lindert ◽  
Marina Krause ◽  
Stephanie Alexander ◽  
Joost te Riet ◽  
...  

Cell migration through 3D tissue depends on a physicochemical balance between cell deformability and physical tissue constraints. Migration rates are further governed by the capacity to degrade ECM by proteolytic enzymes, particularly matrix metalloproteinases (MMPs), and integrin- and actomyosin-mediated mechanocoupling. Yet, how these parameters cooperate when space is confined remains unclear. Using MMP-degradable collagen lattices or nondegradable substrates of varying porosity, we quantitatively identify the limits of cell migration by physical arrest. MMP-independent migration declined as linear function of pore size and with deformation of the nucleus, with arrest reached at 10% of the nuclear cross section (tumor cells, 7 µm2; T cells, 4 µm2; neutrophils, 2 µm2). Residual migration under space restriction strongly depended upon MMP-dependent ECM cleavage by enlarging matrix pore diameters, and integrin- and actomyosin-dependent force generation, which jointly propelled the nucleus. The limits of interstitial cell migration thus depend upon scaffold porosity and deformation of the nucleus, with pericellular collagenolysis and mechanocoupling as modulators.


2011 ◽  
Vol 8 (2) ◽  
pp. 029501-029501 ◽  
Author(s):  
Olga Ilina ◽  
Gert-Jan Bakker ◽  
Angela Vasaturo ◽  
Robert M Hoffman ◽  
Peter Friedl

2011 ◽  
Vol 8 (1) ◽  
pp. 015010 ◽  
Author(s):  
Olga Ilina ◽  
Gert-Jan Bakker ◽  
Angela Vasaturo ◽  
Robert M Hofmann ◽  
Peter Friedl

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