Toxoplasmosis of the central nervous system in the adult. Electron microscopic observations

1978 ◽  
Vol 41 (3) ◽  
pp. 211-216 ◽  
Author(s):  
H. C. Powell ◽  
C. J. Gibbs ◽  
A. M. Lorenzo ◽  
P. W. Lampert ◽  
D. C. Gajdusek
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Electron Microscopic ◽  
The Central Nervous System

Ewing's Family of Tumors Involving Structures Related to the Central Nervous System: A Review

2000 ◽  
Vol 3 (3) ◽  
pp. 203-210 ◽  
Author(s):  
M. Gary Hadfield ◽  
Martha M. Quezado ◽  
Robert L. Williams ◽  
Vivian Y. Luo
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Soft Tissues ◽  
Case Reports ◽  
Spinal Column ◽  
Diagnostic Methods ◽  
Electron Microscopic ◽  
Adjacent Structures ◽  
Microscopic Features ◽  
The Central Nervous System

This review consolidates information gleaned from several case reports and larger series on Ewing's sarcoma family of tumors (EFT) involving structures related to and found in the central nervous system (CNS). These tumors involve the skull, the spinal column, adjacent soft tissues, the meninges, and the brain. We have separated the cases by skull region and spinal column level, and we discuss the attendant differences in prognosis following treatment by neurosurgery, radiation, and chemotherapy. Light and electron microscopic features can be used to differentiate EFT from other small round blue cell tumors that involve the CNS (central primitive neuroectodermal tumor, lymphoma, etc.). Recent molecular and genetic findings in EFT provide new diagnostic methods. We conclude that EFT involving the CNS and adjacent structures is not so rare as previously stated and that the prognosis is more favorable, as a rule, than for the more common examples arising in the long bones and pelvis.

4. Electron Microscopic Studies on the Central Nervous System

1962 ◽  
Vol 4 (0) ◽  
pp. 146-146
Author(s):  
P. H. HASHIMOTO ◽  
T. MAEDA ◽  
S. MORI ◽  
N. SHIMIZU
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
The Central Nervous System

A combined pre- and post-embedding double labeling in the central nervous system with good tissue preservation

1991 ◽  
Vol 49 ◽  
pp. 276-277
Author(s):  
A.M. Milroy ◽  
D.D. Ralston
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Double Labeling ◽  
Highly Sensitive ◽  
The Central Nervous System

Multiple labeling at the electron microscopic level is routinely done in various parts of the central nervous system. We demonstrate that the pre-embedding tetramethylbenzidine (TMB) reaction for visualizing horseradish peroxidase (HRP) of Olucha and the slow osmication of Henry combined with a post-embedding nonetching immunogold method will also preserve good ultrastructure. Furthermore, the post-embedding immunocytochemistry of some neurotransmitters, i.e. gammaaminobutyric acid (GABA), can be done months after the tissue has been reacted for HRP and embedded in regular epon.Pre-embedding histochemistry:The use of TMB as a chromagen for the demonstration of neuronally transported HRP has both the advantage of being highly sensitive and of producing very specific needle-like crystals. Olucha et al demonstrated that one could further stabilize this reaction product with amonium heptamolybdate. Unfortunately the next step, fixation with regular osmium tetroxide, often resulted in the loss of the reaction product. However, the slow osmication with a lower pH (5.5) in the phosphate buffer at room temperature as recommended by Henry et al prevented this loss, and at the same time resulted in well preserved ultrastructure.

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