scholarly journals A Combined Method of Immunocytochemistry of Choline-acetyltransferase Detection and of Autoradiography of (3H)choline after Retrograde Axonal Transport. Study of Cholinergic Pathways in the Central Nervous System at Light and Electron Microscopic Level.

1992 ◽  
Vol 68 (8) ◽  
pp. 145-149 ◽  
Author(s):  
Shigeru TSUJI ◽  
Ion MOTELICA-HEINO ◽  
Marie-Jeanne BRISORGUEIL ◽  
Philippe ANGLADE ◽  
Silvia ARANEDA
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Choline Acetyltransferase ◽  
Retrograde Axonal Transport ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Combined Method ◽  
Electron Microscopic ◽  
Cholinergic Pathways ◽  
The Central Nervous System

A combined pre- and post-embedding double labeling in the central nervous system with good tissue preservation

1991 ◽  
Vol 49 ◽  
pp. 276-277
Author(s):  
A.M. Milroy ◽  
D.D. Ralston
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Double Labeling ◽  
Highly Sensitive ◽  
The Central Nervous System

Multiple labeling at the electron microscopic level is routinely done in various parts of the central nervous system. We demonstrate that the pre-embedding tetramethylbenzidine (TMB) reaction for visualizing horseradish peroxidase (HRP) of Olucha and the slow osmication of Henry combined with a post-embedding nonetching immunogold method will also preserve good ultrastructure. Furthermore, the post-embedding immunocytochemistry of some neurotransmitters, i.e. gammaaminobutyric acid (GABA), can be done months after the tissue has been reacted for HRP and embedded in regular epon.Pre-embedding histochemistry:The use of TMB as a chromagen for the demonstration of neuronally transported HRP has both the advantage of being highly sensitive and of producing very specific needle-like crystals. Olucha et al demonstrated that one could further stabilize this reaction product with amonium heptamolybdate. Unfortunately the next step, fixation with regular osmium tetroxide, often resulted in the loss of the reaction product. However, the slow osmication with a lower pH (5.5) in the phosphate buffer at room temperature as recommended by Henry et al prevented this loss, and at the same time resulted in well preserved ultrastructure.

scholarly journals FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE

1962 ◽  
Vol 12 (2) ◽  
pp. 385-410 ◽  
Author(s):  
Sanford L. Palay ◽  
S. M. McGee-Russell ◽  
Spencer Gordon ◽  
Mary A. Grillo
Keyword(s):  
Electron Microscopy ◽  
Nervous System ◽  
Osmium Tetroxide ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Neural Tissues ◽  
Structural Relations ◽  
Cellular Components

This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.

scholarly journals Inhibition of choline acetyltransferase by excitatory amino acids as a possible mechanism for cholinergic dysfunction in the central nervous system

2001 ◽  
Vol 77 (4) ◽  
pp. 1136-1144 ◽  
Author(s):  
Nelson E. Loureiro-dos-Santos ◽  
Ricardo A. M. Reis ◽  
Regina C. C. Kubrusly ◽  
Olga M. M. S. De Almeida ◽  
Patricia F. Gardino ◽  
...  
Keyword(s):  
Amino Acids ◽  
Central Nervous System ◽  
Nervous System ◽  
Choline Acetyltransferase ◽  
Excitatory Amino Acids ◽  
Cholinergic Dysfunction ◽  
The Central Nervous System

Comparative analysis of the distribution of choline acetyltransferase in the central nervous system of cyprinids

2005 ◽  
Vol 66 (4-6) ◽  
pp. 546-549 ◽  
Author(s):  
Diego Clemente ◽  
Francisco Javier Arenzana ◽  
Rosario Sánchez-González ◽  
Ángel Porteros ◽  
José Aijón ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Comparative Analysis ◽  
Choline Acetyltransferase ◽  
The Central Nervous System

Toxoplasmosis of the central nervous system in the adult. Electron microscopic observations

1978 ◽  
Vol 41 (3) ◽  
pp. 211-216 ◽  
Author(s):  
H. C. Powell ◽  
C. J. Gibbs ◽  
A. M. Lorenzo ◽  
P. W. Lampert ◽  
D. C. Gajdusek
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Electron Microscopic ◽  
The Central Nervous System
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