The study of epithelial function by quantitative light microscopy

1985 ◽  
Vol 405 (S1) ◽  
pp. S23-S27 ◽  
Author(s):  
Kenneth R. Spring
Keyword(s):  
Light Microscopy ◽  
Quantitative Light Microscopy

Development of the pulmonary vasculature in newborn lambs: structure-function relationships

1991 ◽  
Vol 70 (3) ◽  
pp. 1255-1264 ◽  
Author(s):  
R. P. Michel ◽  
J. B. Gordon ◽  
K. Chu
Keyword(s):  
Light Microscopy ◽  
Hypoxic Pulmonary Vasoconstriction ◽  
Venous Pressure ◽  
Pulmonary Arteries ◽  
Muscle Thickness ◽  
Pulmonary Vasculature ◽  
Pressure Gradients ◽  
Middle Segment ◽  
Pulmonary Vasoconstriction ◽  
Quantitative Light Microscopy

Our objectives were 1) to describe the quantitative light microscopy and ultrastructure of newborn lamb lungs and 2) to correlate hemodynamic changes during normoxia and hypoxia with the morphology. By light microscopy, we measured the percent muscle thickness (%MT) and peripheral muscularization of pulmonary arteries and veins from 25 lambs aged less than 24 h, 2-4 days, 2 wk, and 1 mo. At the same ages, lungs were isolated and perfused in situ and, after cyclooxygenase blockade with indomethacin, total, arterial (delta Pa), middle (delta Pm), and venous pressure gradients at inspired O2 fractions of 0.28 (mild hyperoxia) and 0.04 (hypoxia) were determined with inflow-outflow occlusion. During mild hyperoxia, delta Pa and delta Pm fell significantly between 2-4 days and 2 wk, whereas during hypoxia, only delta Pm fell. The %MT of all arteries (less than 50 to greater than 1,000 microns diam) decreased, and peripheral muscularization of less than 100-microns-diam arteries fell between less than 4 days and greater than 2 wk. Our data suggest that 1) the %MT of arteries determines normoxic pulmonary vascular resistance, because only arterial and middle segment resistance fell, 2) peripheral muscularization is a major determinant of hypoxic pulmonary vasoconstriction, because we observed a fall with age in peripheral muscularization of less than 100-micron-diam arteries and in delta Pm with hypoxia, and 3) the arterial limit of the middle segment defined by inflow-outflow occlusion lies in 100- to 1,000-microns-diam arteries.

Quantitative Light Microscopy

1994 ◽  
Vol 21 (3) ◽  
pp. 463-475
Author(s):  
Jeffrey O. Hollinger ◽  
David Buck ◽  
John P. Schmitz
Keyword(s):  
Light Microscopy ◽  
Quantitative Light Microscopy

scholarly journals Calibrating excitation light fluxes for quantitative light microscopy in cell biology

2008 ◽  
Vol 3 (11) ◽  
pp. 1809-1814 ◽  
Author(s):  
David Grünwald ◽  
Shailesh M Shenoy ◽  
Sean Burke ◽  
Robert H Singer
Keyword(s):  
Light Microscopy ◽  
Cell Biology ◽  
Excitation Light ◽  
Quantitative Light Microscopy

scholarly journals Applications of Modern Stereology to Quantitative Light Microscopy

2009 ◽  
Vol 15 (S2) ◽  
pp. 1526-1527 ◽  
Author(s):  
P Mouton
Keyword(s):  
Light Microscopy ◽  
Quantitative Light Microscopy

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

scholarly journals Making microscopy count: quantitative light microscopy of dynamic processes in living plants

2016 ◽  
Vol 263 (2) ◽  
pp. 181-191 ◽  
Author(s):  
MARK D. FRICKER ◽  
JULIAN MOGER ◽  
GEORGE R. LITTLEJOHN ◽  
MICHAEL J. DEEKS
Keyword(s):  
Light Microscopy ◽  
Dynamic Processes ◽  
Quantitative Light Microscopy
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