Comparison of quantitative light microscopy techniques used in diet studies of detritus-consuming omnivores

Hydrobiologia ◽  
1992 ◽  
Vol 239 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Molly O. Ahlgren ◽  
Stephen H. Bowen
Keyword(s):  
Light Microscopy ◽  
Quantitative Light Microscopy ◽  
Microscopy Techniques

2013 Microscopy Today Innovation Awards

2013 ◽  
Vol 21 (5) ◽  
pp. 40-45
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Scanning Probe Microscopy ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Analysis Methods ◽  
Microscopy Analysis ◽  
Ion Microscopy ◽  
Microscopy Techniques

Microscopy Today congratulates the fourth annual group of Innovation Award winners. The ten innovations described below move several microscopy techniques forward: light microscopy, scanning probe microscopy, electron microscopy, ion microscopy, and hybrid microscopy-analysis methods. These innovations will make imaging and analysis more powerful, more flexible, more productive, and easier to accomplish.

Thin-Section Preparation and Transmitted-Light Microscopy

2010 ◽  
pp. 115-135
Keyword(s):  
Light Microscopy ◽  
Polymeric Composites ◽  
Glass Slide ◽  
Transmitted Light ◽  
Fiber Reinforced ◽  
Transmitted Light Microscopy ◽  
Ultrathin Sections ◽  
Microscopy Techniques ◽  
Surface Mounting ◽  
Selection Of

Abstract Transmitted-light methods reveal more details of the morphology of fiber-reinforced polymeric composites than are observable using any other available microscopy techniques. This chapter describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted-light microscopy techniques. The preparation steps covered are a selection of the rough section, preparation of the rough section for preliminary mounting, grinding and polishing the primary-mount first surface, mounting the first surface on a glass slide, and preparing the second surface (top surface). The optimization of microscope conditions and analysis of specimens by microscopy techniques are also covered. In addition, examples of composite ultrathin sections that are analyzed using transmitted-light microscopy contrast methods are shown throughout.

Electron Microscopy Applied to Fine-Needle Aspiration. A Report of Six Cases from Various Sites

1983 ◽  
Vol 69 (5) ◽  
pp. 423-435 ◽  
Author(s):  
Saverio Cinti ◽  
Maurizio Ferretti ◽  
Silvana Amati ◽  
Giancarlo Balercia ◽  
Adalberto Vecchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrastructural Analysis ◽  
Tissue Structure ◽  
Fine Needle ◽  
Neoplastic Lesions ◽  
Microscopy Techniques

The authors report the results obtained from the application of electron microscopy techniques to the cytology of fine-needle-aspirated samples of neoplastic lesions from various body sites. These results show that the tissue structure, which is usually lost during the squashing necessary for light microscopy cytology, is preserved when the samples are processed for ultrastructural analysis. Electron microscopy also allows a highly detailed study of the cell's inner structures. Thus, when this technique is applied, fine needle-aspirated samples can be regarded as actual microbiopsies. However, because of the high cost of ultrastructural techniques, we suggest that actual analysis be performed only in selected cases, whereas fixation and inclusion for electron microscopy could be done routinely.

Development of the pulmonary vasculature in newborn lambs: structure-function relationships

1991 ◽  
Vol 70 (3) ◽  
pp. 1255-1264 ◽  
Author(s):  
R. P. Michel ◽  
J. B. Gordon ◽  
K. Chu
Keyword(s):  
Light Microscopy ◽  
Hypoxic Pulmonary Vasoconstriction ◽  
Venous Pressure ◽  
Pulmonary Arteries ◽  
Muscle Thickness ◽  
Pulmonary Vasculature ◽  
Pressure Gradients ◽  
Middle Segment ◽  
Pulmonary Vasoconstriction ◽  
Quantitative Light Microscopy

Our objectives were 1) to describe the quantitative light microscopy and ultrastructure of newborn lamb lungs and 2) to correlate hemodynamic changes during normoxia and hypoxia with the morphology. By light microscopy, we measured the percent muscle thickness (%MT) and peripheral muscularization of pulmonary arteries and veins from 25 lambs aged less than 24 h, 2-4 days, 2 wk, and 1 mo. At the same ages, lungs were isolated and perfused in situ and, after cyclooxygenase blockade with indomethacin, total, arterial (delta Pa), middle (delta Pm), and venous pressure gradients at inspired O2 fractions of 0.28 (mild hyperoxia) and 0.04 (hypoxia) were determined with inflow-outflow occlusion. During mild hyperoxia, delta Pa and delta Pm fell significantly between 2-4 days and 2 wk, whereas during hypoxia, only delta Pm fell. The %MT of all arteries (less than 50 to greater than 1,000 microns diam) decreased, and peripheral muscularization of less than 100-microns-diam arteries fell between less than 4 days and greater than 2 wk. Our data suggest that 1) the %MT of arteries determines normoxic pulmonary vascular resistance, because only arterial and middle segment resistance fell, 2) peripheral muscularization is a major determinant of hypoxic pulmonary vasoconstriction, because we observed a fall with age in peripheral muscularization of less than 100-micron-diam arteries and in delta Pm with hypoxia, and 3) the arterial limit of the middle segment defined by inflow-outflow occlusion lies in 100- to 1,000-microns-diam arteries.

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