scholarly journals Calibrating excitation light fluxes for quantitative light microscopy in cell biology

2008 ◽  
Vol 3 (11) ◽  
pp. 1809-1814 ◽  
Author(s):  
David Grünwald ◽  
Shailesh M Shenoy ◽  
Sean Burke ◽  
Robert H Singer
Keyword(s):  
Light Microscopy ◽  
Cell Biology ◽  
Excitation Light ◽  
Quantitative Light Microscopy

scholarly journals LS1C2 Introduction to multi-mode light microscopy and its application to cell biology

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S223
Author(s):  
M. Sokabe ◽  
H. Tatsumi
Keyword(s):  
Light Microscopy ◽  
Cell Biology ◽  
Multi Mode

Development of the pulmonary vasculature in newborn lambs: structure-function relationships

1991 ◽  
Vol 70 (3) ◽  
pp. 1255-1264 ◽  
Author(s):  
R. P. Michel ◽  
J. B. Gordon ◽  
K. Chu
Keyword(s):  
Light Microscopy ◽  
Hypoxic Pulmonary Vasoconstriction ◽  
Venous Pressure ◽  
Pulmonary Arteries ◽  
Muscle Thickness ◽  
Pulmonary Vasculature ◽  
Pressure Gradients ◽  
Middle Segment ◽  
Pulmonary Vasoconstriction ◽  
Quantitative Light Microscopy

Our objectives were 1) to describe the quantitative light microscopy and ultrastructure of newborn lamb lungs and 2) to correlate hemodynamic changes during normoxia and hypoxia with the morphology. By light microscopy, we measured the percent muscle thickness (%MT) and peripheral muscularization of pulmonary arteries and veins from 25 lambs aged less than 24 h, 2-4 days, 2 wk, and 1 mo. At the same ages, lungs were isolated and perfused in situ and, after cyclooxygenase blockade with indomethacin, total, arterial (delta Pa), middle (delta Pm), and venous pressure gradients at inspired O2 fractions of 0.28 (mild hyperoxia) and 0.04 (hypoxia) were determined with inflow-outflow occlusion. During mild hyperoxia, delta Pa and delta Pm fell significantly between 2-4 days and 2 wk, whereas during hypoxia, only delta Pm fell. The %MT of all arteries (less than 50 to greater than 1,000 microns diam) decreased, and peripheral muscularization of less than 100-microns-diam arteries fell between less than 4 days and greater than 2 wk. Our data suggest that 1) the %MT of arteries determines normoxic pulmonary vascular resistance, because only arterial and middle segment resistance fell, 2) peripheral muscularization is a major determinant of hypoxic pulmonary vasoconstriction, because we observed a fall with age in peripheral muscularization of less than 100-micron-diam arteries and in delta Pm with hypoxia, and 3) the arterial limit of the middle segment defined by inflow-outflow occlusion lies in 100- to 1,000-microns-diam arteries.

scholarly journals A microscopic view of the cell

2016 ◽  
Vol 27 (21) ◽  
pp. 3183-3184
Author(s):  
Bo Huang
Keyword(s):  
Quantitative Analysis ◽  
Light Microscopy ◽  
Cell Biology ◽  
Integrative Approach ◽  
Biological Knowledge ◽  
Critical Links ◽  
Indispensable Tool ◽  
Biology Research

Light microscopy has long been an indispensable tool for cell biology research. From biological problems to biological knowledge, there are two more critical links in the light microscopy approach: labeling and quantitative analysis. Therefore, an integrative approach is desirable in order to deal with practical challenges in biological light microscopy.

scholarly journals Genetically encoded fluorescent tags

2017 ◽  
Vol 28 (7) ◽  
pp. 848-857 ◽  
Author(s):  
Kurt Thorn
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Light Microscopy ◽  
Cell Biology ◽  
Fluorescent Proteins ◽  
Protein Sequences ◽  
Biological Properties ◽  
Protein Abundance ◽  
Fluorescent Tags ◽  
Exogenous Fluorophores

Genetically encoded fluorescent tags are protein sequences that can be fused to a protein of interest to render it fluorescent. These tags have revolutionized cell biology by allowing nearly any protein to be imaged by light microscopy at submicrometer spatial resolution and subsecond time resolution in a live cell or organism. They can also be used to measure protein abundance in thousands to millions of cells using flow cytometry. Here I provide an introduction to the different genetic tags available, including both intrinsically fluorescent proteins and proteins that derive their fluorescence from binding of either endogenous or exogenous fluorophores. I discuss their optical and biological properties and guidelines for choosing appropriate tags for an experiment. Tools for tagging nucleic acid sequences and reporter molecules that detect the presence of different biomolecules are also briefly discussed.

Electron Microscopy and cell biology in the 20th century

1992 ◽  
Vol 50 (1) ◽  
pp. 8-9
Author(s):  
Keith R. Porter
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Biology ◽  
Culture Medium ◽  
Specimen Preparation ◽  
Distilled Water ◽  
Access To Information ◽  
History Of ◽  
A Cell

The recent history of cell biology cannot be recorded without generous reference to electron microscopy. After all, it was this instrument and the enormous increase in resolution it provided that gave cell biologists access to information that even the most imaginative of investigators had not submitted to the printed page. This paper will record highlights that a few investigators introduced into specimen preparation. This pursuit has contributed more to the progress, i.e. history, of the field than is generally recognized. The first images of cells that can be said to reveal something new were published in 1945. They were micrographs of thinly spread cells grown in tissue culture on surfaces coated with formvar and thence with evaporated carbon. After the suitability of a cell or group of cells had been established by light microscopy, they were freed of culture medium with balanced salt solution and fixed with OsO4. Thereafter, and before drying, the selected cells were washed in distilled water, and to get the cells onto a grid, a small flap of the formvar film was separated from the glass surface and floated over the grid. Subsequently, the preparation was drained of H2O on filter paper and allowed to dry. Cells that resided in open areas between the wires of the grid were available for examination.

Quantitative Light Microscopy

1994 ◽  
Vol 21 (3) ◽  
pp. 463-475
Author(s):  
Jeffrey O. Hollinger ◽  
David Buck ◽  
John P. Schmitz
Keyword(s):  
Light Microscopy ◽  
Quantitative Light Microscopy
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