Diffusion barrier to horseradish peroxidase in the vascular stria of the guinea pig

1971 ◽  
Vol 121 (4) ◽  
pp. 499-506 ◽  
Author(s):  
Finn �. Winther
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig ◽  
Diffusion Barrier ◽  
Vascular Stria

scholarly journals ASSAY OF ANTIHORSERADISH PEROXIDASE ANTIBODY IN AN IMMUNOCYTOCHEMICAL MODEL SYSTEM OF POLYACRYLAMIDE GEL FILMS

1973 ◽  
Vol 21 (9) ◽  
pp. 779-784 ◽  
Author(s):  
SADAKI YOKOTA
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig ◽  
Model System ◽  
Polyacrylamide Gel ◽  
Antibody Activity

The effects of fixatives on the antibody and horseradish peroxidase (HRP) activities in the immunocytochemical system were investigated quantitatively by using a model of polyacrylamide gel films containing proteins such as goat γ-globulin, anti-HRP guinea pig antiserum or HRP. The activity of the anti-HRP antibody was measured indirectly by staining the HRP coupled with its antibody in the gel films. About 40% of the antibody activity was lost by fixation in 2.5% glutaraldehyde and approximately 35% in 4% formalin. The inactivation of HRP by the refixation in glutaraldehyde or formalin was also examined. The loss of HRP activity was inversely related to the concentration of the fixatives. The fixation in 2.5% glutaraldehyde and 4% formalin inhibited HRP activity 35% and 40%, respectively. The inactivation occurred generally within 40 min of the refixation.

Vascular reactions to horseradish peroxidase in the guinea pig

1977 ◽  
Vol 53 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Torgeir Vegge ◽  
Rolf Haye
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig

Role of the epithelium in airway smooth muscle responses to relaxant agonists

1991 ◽  
Vol 71 (4) ◽  
pp. 1434-1440 ◽  
Author(s):  
J. N. Yang ◽  
W. Mitzner ◽  
C. Hirshman
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Diffusion Barrier ◽  
Tracheal Wall ◽  
Maximal Effect ◽  
Direct Measurements ◽  
Dose Dependent ◽  
Muscle Responses

We studied the role of the guinea pig tracheal epithelium in modulating tracheal smooth muscle responses to the relaxant agonists albuterol, sodium nitroprusside, and theophylline. We used an in vitro preparation that allowed separation of the fluids bathing the luminal (internal) and serosal (external) surfaces of the trachea, and bronchodilators were administered to either surface of carbachol-contracted tracheae. All three drugs produced dose-dependent relaxation. However, albuterol and nitroprusside were less potent (concentration that produced half-maximal effect increased by 100- and 32-fold, respectively) when given to the epithelial side with the epithelium intact compared with the epithelium denuded or compared with serosal administration with the epithelium intact. These differences were not observed for theophylline, where smooth muscle responses were independent of either the side of stimulation or of the presence or absence of the epithelium. Direct measurements of the diffusion of theophylline across the tracheal wall in the presence or absence of epithelium showed that after 5 h of incubation with a fixed luminal concentration of theophylline, only 1.7% had diffused across the tracheal wall with the epithelium intact. This increased to only approximately 3.3% when the epithelium was denuded. These results suggest that the epithelial is a relatively weak barrier for lipophilic agents but has a major role as a diffusion barrier to hydrophilic substances.

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