Vascular reactions to horseradish peroxidase in the guinea pig

1977 ◽  
Vol 53 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Torgeir Vegge ◽  
Rolf Haye
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig

scholarly journals ASSAY OF ANTIHORSERADISH PEROXIDASE ANTIBODY IN AN IMMUNOCYTOCHEMICAL MODEL SYSTEM OF POLYACRYLAMIDE GEL FILMS

1973 ◽  
Vol 21 (9) ◽  
pp. 779-784 ◽  
Author(s):  
SADAKI YOKOTA
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig ◽  
Model System ◽  
Polyacrylamide Gel ◽  
Antibody Activity

The effects of fixatives on the antibody and horseradish peroxidase (HRP) activities in the immunocytochemical system were investigated quantitatively by using a model of polyacrylamide gel films containing proteins such as goat γ-globulin, anti-HRP guinea pig antiserum or HRP. The activity of the anti-HRP antibody was measured indirectly by staining the HRP coupled with its antibody in the gel films. About 40% of the antibody activity was lost by fixation in 2.5% glutaraldehyde and approximately 35% in 4% formalin. The inactivation of HRP by the refixation in glutaraldehyde or formalin was also examined. The loss of HRP activity was inversely related to the concentration of the fixatives. The fixation in 2.5% glutaraldehyde and 4% formalin inhibited HRP activity 35% and 40%, respectively. The inactivation occurred generally within 40 min of the refixation.

The uptake of horseradish peroxidase by the conjunctival epithelium of the guinea-pig

1987 ◽  
Vol 45 (2) ◽  
pp. 327-337 ◽  
Author(s):  
E. Lee Stock ◽  
Robert A. Sobut ◽  
Sanford I. Roth
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig ◽  
Conjunctival Epithelium

Langerhans Cell Granule as Phagocytic Organelle

1970 ◽  
Vol 28 ◽  
pp. 96-97
Author(s):  
Ken Hashimoto
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Guinea Pig ◽  
Normal Saline ◽  
Functional Significance ◽  
Langerhans Cell ◽  
Cell Granule ◽  
Normal Adult ◽  
Cacodylate Buffer

In recent years the epidermal Langerhans cell has been considered to belong to histiocytic rather than melanocytic lineage. However, the functional significance of the Langerhans cell granule, which has also been called Bribeck's granule or racket body, has not been settled. In this study, 0.1 ml of horseradish peroxidase solution (25 mg in 1.0 ml normal saline) was injected intradermally into six sites on the back of a normal adult guinea pig. The injected sites were biopsied at 10, 30, 60, 120, 180 and 300 minutes and later at 22, 120 and 142 hours. The epidermis was minced with sharp razor blades into 1 mm blocks and fixed in 5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2) for 3 hours at 4°C. After overnight rinse in the same buffer, tissue blocks were minced again into smaller pieces to facilitate the penetration of benzidine and hydrogen peroxide. Benzidine reaction was done according to the method of Schneeberger-Keeley and Karnowsky.

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